Macrophage mediated mesoscale brain mechanical homeostasis mechanically imaged via optical tweezers and Brillouin microscopy in vivo.

Tissues are active materials where epithelial turnover, immune surveillance, and remodeling of stromal cells such as macrophages all regulate form and function. Scattering modalities such as Brillouin microscopy (BM) can non-invasively access mechanical signatures at GHz. However, our traditional understanding of tissue material properties is derived mainly from modalities which probe mechanical properties at different frequencies. Thus, reconciling measurements amongst these modalities remains an active area. Here, we compare optical tweezer active microrheology (OT-AMR) and Brillouin microscopy (BM) to longitudinally map brain development in the larval zebrafish. We determine that each measurement is able to detect a mechanical signature linked to functional units of the brain. We demonstrate that the corrected BM-Longitudinal modulus using a density factor correlates well with OT-AMR storage modulus at lower frequencies. We also show that the brain tissue mechanical properties are dependent on both the neuronal architecture and the presence of macrophages. Moreover, the BM technique is able to delineate the contributions to mechanical properties of the macrophage from that due to colony stimulating factor 1 receptor (CSF1R) mediated stromal remodeling. Here, our data suggest that macrophage remodeling is instrumental in the maintenance of tissue mechanical homeostasis during development. Moreover, the strong agreement between the OT-AM and BM further demonstrates that scattering-based technique is sensitive to both large and minute structural modification in vivo.

The effect of bio-banding on technical and tactical indicators of talent identification in academy soccer players.

AIM: The aim of this study was to examine the effect of bio-banding on technical and tactical markers of talent identification in 11- to 14-year-old academy soccer players. METHODS: Using a repeated measures design, 92 players were bio-banded using percentage of estimated adult stature attainment (week 1), maturity-offset (week 2) and a mixed-maturity method (week 3). All players contested five maturity (mis)matched small-sided games with technical and tactical variables measured. Data were analysed using a series of Bayesian hierarchical models, fitted with different response distributions and different random and fixed effect structures. RESULTS: Despite differences during maturity-matched bio-banding for post-peak height velocity players, very few tactical differences were evident during the remaining maturity-matched and mis-matched fixtures for both banding methods. In fact, the results showed no consistent differences across both banding methods for practitioner and video analysis-derived technical performance characteristics during maturity matched and mis-matched fixtures. Both bio-banding methods explained similar levels of variance across the measured variables. CONCLUSION: Maturity-matched bio-banding had some effect on both technical and tactical characteristics of players during maturity-matched bio-banded formats. That said, this trend remained during maturity mis-matched bio-banded formats which restricts the conclusions that can be made regarding the effectiveness of bio-banding to manipulate technical and tactical measures in academy soccer players.

The effect of bio-banding on physical and psychological indicators of talent identification in academy soccer players.

The aim of this study was to examine the effect of bio-banding on indicators of talent identification in academy soccer players. Seventy-two 11 to 14-year-old soccer players were bio-banded using percentage of estimated adult stature attainment (week 1), maturity-offset (week 2) or a mixed-maturity method (week 3). Players contested five maturity (mis)matched small-sided games with physical and psychological determinants measured. Data were analysed using a series of Bayesian hierarchical models, fitted with different response distributions and different random and fixed effect structures. Few between-maturity differences existed for physical measures. Pre-peak height velocity (PHV) and post-PHV players differed in PlayerLoadTM (anterior-posterior and medial-lateral) having effect sizes above our criterion value. Estimated adult stature attainment explained more of the variance in eight of the physical variables and showed the greatest individual differences between maturity groups across all psychological variables. Pre-PHV and post-PHV players differed in positive attitude, confidence, competitiveness, total psychological score (effect sizes = 0.43-0.69), and session rating of perceived exertion. The maturity-offset method outperformed the estimated adult stature attainment method in all psychological variables. Maturity-matched bio-banding had limited effect on physical variables across all players while enhancing a number of psychological variables considered key for talent identification in pre-PHV players.

Independent Control of Topography for 3D Patterning of the ECM Microenvironment.

Biomimetic extracellular matrix (ECM) topographies driven by the magnetic-field-directed self-assembly of ECM protein-coated magnetic beads are fabricated. This novel bottom-up method allows us to program isotropic, anisotropic, and diverse hybrid ECM patterns without changing other physicochemical properties of the scaffold material. It is demonstrated that this 3D anisotropic matrix is able to guide the dendritic protrusion of cells.

Mismatch in mechanical and adhesive properties induces pulsating cancer cell migration in epithelial monolayer.

The mechanical and adhesive properties of cancer cells significantly change during tumor progression. Here we assess the functional consequences of mismatched stiffness and adhesive properties between neighboring normal cells on cancer cell migration in an epithelial-like cell monolayer. Using an in vitro coculture system and live-cell imaging, we find that the speed of single, mechanically soft breast carcinoma cells is dramatically enhanced by surrounding stiff nontransformed cells compared with single cells or a monolayer of carcinoma cells. Soft tumor cells undergo a mode of pulsating migration that is distinct from conventional mesenchymal and amoeboid migration, whereby long-lived episodes of slow, random migration are interlaced with short-lived episodes of extremely fast, directed migration, whereas the surrounding stiff cells show little net migration. This bursty migration is induced by the intermittent, myosin II-mediated deformation of the soft nucleus of the cancer cell, which is induced by the transient crowding of the stiff nuclei of the surrounding nontransformed cells, whose movements depend directly on the cadherin-mediated mismatched adhesion between normal and cancer cells as well as α-catenin-based intercellular adhesion of the normal cells. These results suggest that a mechanical and adhesive mismatch between transformed and nontransformed cells in a cell monolayer can trigger enhanced pulsating migration. These results shed light on the role of stiff epithelial cells that neighbor individual cancer cells in early steps of cancer dissemination.