RESUMO
BACKGROUND: The IFA test is one of the most usual methods for detecting anti-Toxoplasma antibodies, although it has not any unique standardization. It seems that the microscopic judgment of results is an important confounder in IFA test. Therefore, we conducted the present study to clarify the role of microscopic observer, and other confounders on the test. METHODS: Eighty sera were collected from patients suspicious to toxoplasmosis for detection IgG anti-T. gondii by this test. Samples were examined against different series of antigens, IgG anti-human conjugates, and observers. RESULTS: There were no significant differences between the two series of antigens and conjugates. For the observers groups the kappa coefficient of the test results in the experts group (0.97, 0.94-1.00) were significantly higher than the less experienced observers (0.77, 0.68-0.87). CONCLUSION: We recommend the IFA test to be performed only in reference laboratories and by laboratory technicians that have enough experience for this test. Otherwise, we suggest the substitution of this test with other tests like ELISA for the diagnosis and epidemiological studies.
RESUMO
In a cross-sectional study, we evaluated the seroprevalence of Toxoplasma gondii among 400 women referred to Qazvin community health centre laboratory for pre-marriage examinations. Indirect immunofluorescent antibody test was used to detect IgG anti-toxoplasma. Titres >/= 1: 20 were considered positive. The overall seropositivity was 34%. Mean age was significantly higher in seropositive women [P < 0.05]. Seropositivity was highest among unemployed women [38.3%] and lowest among students [22.6%], and was significantly higher in women with less than high-school education [P < 0.05]. With two-thirds of these unmarried women seronegative, they represent a high-risk group in pregnancy. Such women need to be educated to prevent congenital toxoplasmosis
