Colorimetric indicator films based on carboxymethyl cellulose and anthocyanins as a visual indicator for shrimp freshness tracking.

This study aimed to evaluate the response efficiency of colorimetric indicator films based on carboxymethyl cellulose (CMC) incorporated with different anthocyanins [Karanda alone (CMC/AK), butterfly pea alone (CMC/AB), and a mixture of anthocyanins from Karanda and butterfly pea (CMC/AK75/AB25)] for tracking shrimp freshness during storage at different temperatures and times (4 °C for 8 days and 25 °C for 30 h). The mathematical models were also applied to predict their freshness and shelf life. The CMC/AK75/AB25 indicator film was the most sensitive and clearly changed color, which could be distinguished by the naked eye. Color changes indicated the shrimp deterioration processes: dark purple (fresh), purplish gray or gray (semi-fresh), and olive green or brown (spoilage). During shrimp storage at temperatures of 4 and 25 °C, the pH reached 7.52 and 8.14, TVB-N 35.98 and 72.72 mg/100 g, and TVC 5.75 and 7.88 log CFU/g, respectively, indicating shrimp had completely deteriorated. Furthermore, there was a positive correlation between the ΔE value of the indicator film and both TVB-N and TVC. These findings suggest that the CMC/AK75/AB25 indicator film could serve as a real-time visual indicator for tracking shrimp freshness and could enhance the guarantee of shrimp safety.

Modelling and assessing new SME digital business status for visualising virtual economics and sustainability economic indicators: Empirical evidence from poultry business.

SMEs are generally recognised as financial contributors to regional economic development. Despite the enormous contributions of digital SMEs to sustainable economic growth and regional development have not yet been reported. This paper assesses and models SME businesses to gain a digital business status and model. These days, visualising economic growth is challenging and powerful to identify business performance and realise sustainable regional development. This research analyses empirical data obtained from a poultry business to form a standard model for implementing a new SME digital business model and status. This standard model is a guideline to measure the other SME businesses and productions. Localisation of the SDGs was analysed using thematic analysis. Mathematical computation is used to visualise virtual economic growth geographically. Hence, our digital business model and status demonstrated business performance, management, and economic growth.

Smart colorimetric sensing films based on carboxymethyl cellulose incorporated with a natural pH indicator.

This study aimed to combine various natural pH indicators of anthocyanin from Karanda (CA) with anthocyanin from butterfly pea flower (BA) or curcumin (CC) to improve the sensitivity of CA. CA75/BA25 and CA25/CC75 enhanced the sensitivity of the endpoint colour change of CA. A smart colorimetric sensing film was also developed and characterised by loading different natural pH indicators on carboxymethyl cellulose (CMC) films. The addition of different natural pH indicators increased the thickness, elongation, colour (a* and b* values), and contact angle of CMC films (p < 0.05). However, the tensile strength, water vapour permeability, film solubility, light transmission, and L* value decreased when different natural pH indicators were added (p < 0.05). The indicator films demonstrated enhanced antioxidant capacity and thermal stability. The FTIR spectra showed that natural pH indicators were successfully immobilised into the CMC films. Notably, the CMC/CA75/BA25 film was the most sensitive film to changes in volatile ammonia and different pH buffer solutions. The CMC/CA75/BA25 film changed from purple to green with exposure to ammonia solution and from pink to purple to blue to green with increasing pH. Therefore, the CMC/CA75/BA25 film has potential as a colorimetric sensing film, providing a more accurate assessment result.

Physicochemical Properties of Chitosan from Green Mussel Shells (Perna viridis): A Comparative Study.

Green mussel shells (Perna viridis) are generated in huge amounts and discarded as waste materials. Such waste may be used to produce biopolymer materials such as chitosan. The physicochemical properties of chitosan prepared from different sizes of green mussel shells (small size (CHS): ≤5.00 cm in length and big size (CHB): >5.01 cm in length) were characterized and compared with commercial chitosan (CH). Furthermore, the mechanical and physicochemical properties of the blended films were also investigated. The results of the physicochemical properties showed that CHS and CHB were quite different from CH. The degree of deacetylation of CHS, CHB, and CH was found to be 32.71%, 52.56%, and 70.42%, respectively (p < 0.05). The water- and fat-binding capacities of CH were higher than those of CHS and CHB. Structural differences between CHS, CHB, and CH were studied using Fourier transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD). Significant increases in thickness, water vapor permeability, and strength of the blended films were found when the extracted chitosan was added (p < 0.05). However, further study is needed to improve the chitosan extraction process, which can enhance the physicochemical properties of the obtained chitosan and be widely used in many industries.

Extraction and Physico-Chemical Characterization of Chitosan from Mantis Shrimp (Oratosquilla nepa) Shell and the Development of Bio-Composite Film with Agarose.

Mantis shrimp (Oratosquilla nepa) exoskeleton, a leftover generated after processing, was used as a starting material for chitosan (CS) production. CS was extracted with different deacetylation times (2, 3 and 4 h), termed CS−2, CS−3 and CS−4, respectively, and their characteristics and antimicrobial and film properties with agarose (AG) were investigated. Prolonged deacetylation time increased the degree of deacetylation (DDA: 73.56 ± 0.09−75.56 ± 0.09%), while extraction yield (15.79 ± 0.19−14.13 ± 0.09%), intrinsic viscosity (η: 3.58 ± 0.09−2.97 ± 0.16 dL/g) and average molecular weight (Mν: 1.4 ± 0.05−1.12 ± 0.08 (×106 Da)) decreased (p < 0.05). FTIR spectra of extracted CS were similar to that of commercial CS. Among all the CS samples prepared, CS−3 had the best yield, DDA, Mν and antimicrobial activity. Therefore, it was chosen for the development of composite films with AG at different ratios (CS−3/AG; 100/0, 75/25, 50/50, 25/75 and 0/100). As the proportion of AG increased, the tensile strength (29.96 ± 1.80−89.70 ± 5.08 MPa) of the composite films increased, while thickness (0.056 ± 0.012−0.024 ± 0.001 mm), elongation at break (36.52 ± 1.12−25.32 ± 1.23%) and water vapor permeability (3.56 ± 0.10−1.55 ± 0.02 (×10−7 g m m−2 s−1 Pa−1)) decreased (p < 0.05). Moreover, lightness of the films increased and yellowness decreased. CS−3/AG (50/50) composite film exhibited high mechanical and barrier properties and excellent compatibility according to FTIR and SEM analyses. According to these finding, mantis shrimp exoskeleton could be used to produce CS. The developed bio-composite film based on an appropriate ratio (50/50) of CS−3 and AG has potential for being used as food packaging material.

Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.

Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application. Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem. The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties. The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion. Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films. Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05). However, the addition of RF in different proportions did not influence the film thickness and transparency. Based on SEM micrographs, all film samples had a relatively coarser surface. FTIR spectra showed that some interactions between CMCy and RF blended films had occurred. According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.

Application of Physical Gas Absorbers in Manipulating the CO2 Pressure of Kimchi Package.

Physical gas absorption and release of activated carbon or zeolite were used to attain the desired CO2 concentration profile inside a package of kimchi for its dynamic storage and consumption environment. The CO2 adsorption/desorption behavior of different combinations of physical scavengers in the microporous Tyvek sachet was measured at 0, 10 and 20 °C. A combination of highly permeable microporous Tyvek and much less permeable nylon/polyethylene laminate was also examined as a variable to help control the access of CO2 to the physical CO2 absorber. The composition of the absorber and its packing density in the sachet affected the CO2 absorption rate and capacity. Increased zeolite in the Tyvek sachet showed higher CO2 absorption followed by higher CO2 release concomitant with moisture sorption, while activated carbon itself had low CO2 absorption and little subsequent CO2 desorption. Lower temperatures increased the capacity of CO2 absorption and release along with lower CO2 absorption and release rates. A reduction in Tyvek area could decrease the absorption and release rates. An application of a physical CO2 absorber to the kimchi package was tested at 10 °C to show potential for keeping the desired CO2 pressure profile for carbonic cool flavor under tolerable pressure during storage and use. PRACTICAL APPLICATIONS: Physical carbon dioxide absorbers consisting of zeolite and activated carbon inside the gas-permeable sachet can be placed in kimchi packages for time-dependent absorption and release of carbon dioxide, which responds in harmony with the fermentation profile of kimchi. Its practical potential is both to provide a carbonic cool taste of the product and to avoid excessive overpressure in the package through its shelf life when used in kimchi packaging. Its intelligent use may resolve the problem of a dull kimchi taste, which occurs when incorporating calcium hydroxide as the chemical scavenger in the package.