Post-mortem investigation of calcium content in liver, heart, and skeletal muscle in accidental hypothermia cases.

The identification of hypothermia as cause of the death was always quite problematic in the field of forensic medicine. The aim of the present study was to verify the determination of calcium content in post-mortem liver, heart, and skeletal muscle samples as the biochemical marker defining hypothermia as the cause of death. The study involved 43 autopsy cases in which the circumstances of death indicated the effects of overcooling. The control group consisted of material collected from the corpses of 30 persons who were not exposed to low temperatures but died due to technical injuries (n=5), asphyxia (n=6), intoxication with ethanol and other substances (n=8), and acute myocardial infarction/ischemia (n=11). The concentration of calcium in autopsy samples was determined applying flame atomic absorption spectroscopy. Our study showed no significant differences of calcium content in tissues of persons who died due to hypothermia, over those who died in normothermic conditions.

Investigation of calcium and sodium in postmortem material as biochemical markers defining the cause of death from hypothermia.

The present investigation was undertaken to evaluate the usefulness of calcium and sodium measurements in the heart, liver, and muscle autopsy samples as biochemical markers defining the cause of death from hypothermia. The concentration of electrolytes in selected internal organ tissues of persons who died from hypothermia (n=26) was compared with element concentrations in analogous tissues of persons that died due to other causes (control group, n=26). Our study showed no significant differences in the calcium content of tissues in persons who died from hypothermia, over those who died in normothermic conditions. The concentration of sodium was significantly (p<0.001) increased in the skeletal muscle of subjects who died from hypothermia.

Application of atomic absorption spectroscopy for detection of multimetal traces in low-voltage electrical marks.

Application of atomic absorption spectroscopy to detect multimetal traces in injured skin is a promising tool for investigation of fatalities caused by electrocution. The present paper is aimed at testing the reliability of this method for metal traces detection in electric current marks and is focused on study of peculiarities of metal penetration into the skin exposed to a current impact. Bare aluminum wire, tin-lead coated copper multistrand wire, and zinc-plated steel rope were used to make electrical marks on pig skin. It is demonstrated that amount of copper, zinc, lead, and iron may serve as statistically reliable indicators for the type of wire, which caused the electrical mark, in spite of the background content of these metals in the skin without injury. Different penetration rates for different metals contained in the wire inflicting an electrical mark were observed.