Neuroprotective and Neurite Outgrowth Stimulating Effects of New Low-Basicity 5-HT7 Receptor Agonists: In Vitro Study in Human Neuroblastoma SH-SY5Y Cells.

There is some evidence that the serotonin receptor subtype 7 (5-HT7) could be new therapeutic target for neuroprotection. The aim of this study was to compare the neuroprotective and neurite outgrowth potential of new 5-HT7 receptor agonists (AH-494, AGH-238, AGH-194) with 5-CT (5-carboxyamidotryptamine) in human neuroblastoma SH-SY5Y cells. The results revealed that 5-HT7 mRNA expression was significantly higher in retinoic acid (RA)-differentiated cells when compared to undifferentiated ones and it was higher in cell cultured in neuroblastoma experimental medium (DMEM) compared to those placed in neuronal (NB) medium. Furthermore, the safety profile of compounds was favorable for all tested compounds at concentration used for neuroprotection evaluation (up to 1 µM), whereas at higher concentrations (above 10 µM) the one of the tested compounds, AGH-194 appeared to be cytotoxic. While we observed relatively modest protective effects of 5-CT and AH-494 in UN-SH-SY5Y cells cultured in DMEM, in UN-SH-SY5Y cells cultured in NB medium we found a significant reduction of H2O2-evoked cell damage by all tested 5-HT7 agonists. However, 5-HT7-mediated neuroprotection was not associated with inhibition of caspase-3 activity and was not observed in RA-SH-SY5Y cells exposed to H2O2. Furthermore, none of the tested 5-HT7 agonists altered the damage induced by 6-hydroxydopamine (6-OHDA), 1-methyl-4-phenylpyridinium ion (MPP +) and doxorubicin (Dox) in UN- and RA-SH-SY5Y cells cultured in NB. Finally we showed a stimulating effect of AH-494 and AGH-194 on neurite outgrowth. The obtained results provide insight into neuroprotective and neurite outgrowth potential of new 5-HT7 agonists.

Influence of Eu3+ Doping on Physiochemical Properties and Neuroprotective Potential of Polyacrylic Acid Functionalized Cerium Oxide Nanoparticles.

Cerium oxide nanoparticles (CeONPs) exhibiting antioxidant properties are investigated as potential tools for neurodegenerative diseases. Here, we synthesized polyacrylic acid conjugated cerium oxide (CeO) nanoparticles, and further to enhance their neuroprotective effect, Eu3+ was substituted at different concentrations (5, 10, 15 and 20 mol%) to the CeO, which can also impart fluorescence to the system. CeONPs and Eu-CeONPs in the size range of 15-30 nm were stable at room temperature. The X-ray Photoelectron Spectroscopy (XPS) analysis revealed the chemical state of Eu and Ce components, and we could conclude that all Eu3+ detected on the surface is well integrated into the cerium oxide lattice. The emission spectrum of Eu-CeO arising from the 7F0 → 5D1 MD and 7F0 → 5D2 transitions indicated the Eu3+ ion acting as a luminescence center. The fluorescence of Eu-CeONPs was visualized by depositing them at the surface of positively charged latex particles. The developed nanoparticles were safe for human neuronal-like cells. Compared with CeONPs, Eu-CeONPs at all concentrations exhibited enhanced neuroprotection against 6-OHDA, while the protection trend of Eu-CeO was similar to that of CeO against H2O2 in SH-SY5Y cells. Hence, the developed Eu-CeONPs could be further investigated as a potential theranostic probe.

A mixture of persistent organic pollutants detected in human follicular fluid increases progesterone secretion and mitochondrial activity in human granulosa HGrC1 cells.

Disruption of granulosa cells (GCs), the main functional cells in the ovary, is associated with impaired female fertility. Epidemiological studies demonstrated that women have detectable levels of organic pollutants (e.g., perfluorooctanoate, perfluorooctane sulfonate, 2,2-dichlorodiphenyldichloroethylene, polychlorinated biphenyl 153, and hexachlorobenzene) in their follicular fluid (FF), and thus these compounds may directly affect the function of GCs in the ovary. Considering that humans are exposed to multiple pollutants simultaneously, we elucidated the effects of a mixture of endocrine-disrupting chemicals (EDCs) on human granulosa HGrC1 cells. The EDC mixture directly increased progesterone secretion by upregulating 3ß-hydroxysteroid dehydrogenase (3ßHSD) expression. Furthermore, the EDC mixture increased activity of mitochondria, which are the central sites for steroid hormone biosynthesis, and the ATP content. Unexpectedly, the EDC mixture reduced glucose transporter 4 (GLUT4) expression and perturbed glucose uptake; however, this did not affect the glycolytic rate. Moreover, inhibition of GLUT1 by STF-31 did not alter the effects of the EDC mixture on steroid secretion but decreased basal estradiol secretion. Taken together, our results demonstrate that the mixture of EDCs present in FF can alter the functions of human GCs by disrupting steroidogenesis and may thus adversely affect female reproductive health. This study highlights that the EDC mixture elicits its effects by targeting mitochondria and increases mitochondrial network formation, mitochondrial activity, and expression of 3ßHSD, which is associated with the inner mitochondrial membrane.

Religiosity and Attitudes towards Health, Disease, Death and the Use of Stimulants among Jehovah's Witnesses.

Religiosity is considered as one of the many factors shaping an individual's health consciousness. The aim of the study is an analysis of the correlation between the religiosity of Jehovah's Witnesses and their attitudes towards health and disease. A cross-sectional study was performed on the convenience sample of 171 Jehovah's Witnesses from eastern Poland with the use of two research tools: the author's questionnaire, focusing on attitudes towards health, disease, death and the use of stimulants, and the Duke University Religion Index (DUREL). The research involved 99 females (57.9%) and 72 males (42.1%), with an average age of 37.25 (SD = 12.59) years. On average, they have been a Jehovah's Witness for 29.21 (SD = 13.22) years and are characterised by a high ratio of organisational religious activity (ORA) (M = 5.60; SD = 0.62) and intrinsic religiosity (IR) (M = 4.81; SD = 0.37). Those who had never smoked before becoming one of Jehovah's Witnesses had a higher IR (Z = -2.822; p = 0.005), similarly to those respondents who smoked cigarettes before they became Jehovah's Witnesses (Z = -2.977; p = 0.003) and those who did not abuse alcohol before they became Jehovah's Witnesses (Z= -1.974; p = 0.048). Jehovah's Witnesses are a group characterised by a high degree of consistency when it comes to religiosity, attitudes regarding health and disease and health behaviours. This means that they follow the teachings of their religion with regard to health issues. Knowledge about the association between religiosity and health behaviours is important to provide effective health education, health promotion and development of health prevention policy, specifically when dealing with more religious groups of clients.

Place of Residence and Marital Status as Variables Differentiating a Sense of Self-Efficacy in the Elderly-A Descriptive Cross-Sectional Survey.

Background: Studies on the self-efficacy of the elderly are still being conducted to a very limited extent. Nevertheless, they can provide relevant information for specialists to use in many disciplines of science, as well as for practitioners, especially gerontologists, geriatricians, psychologists and other people associated with prophylaxis and health promotion. The aim of the study is to assess the impact of the place of residence and marital status on the self-efficacy in the elderly. Methods: A diagnostic survey method was used for the study and within the method, the authors' sociodemographic data questionnaire and the generalized self-efficacy scale (GSES) were applied. The study was carried out in a group of 171 females and 130 males under nursing care in the home environment or in nursing-home care in Poland. Results: The average level of generalized sense of self-efficacy in the research cohort was M = 26.69, SD = 8.49. A relatively high percentage of the group (48.9%) showed a sense of self-efficacy at a high level. The level of a sense of self-efficacy was low in 38.2% of the cohort, while its average intensity in the remaining ones constituted 12.9% of the total. A statistically significant impact of a place of residence (Z = -2.940; p = 0.003) and marital status (H = 12.000; p = 0.007) on the sense of efficacy in the research cohort was proven in favor of those from the rural environment and the respondents having spouses. Conclusions: On the scale of the studied group, the results of a sense of self-efficacy are optimistic, as older people with high levels predominated. However, the results of the cohort whose self-efficacy is insufficient to cope with their old age should be considered carefully, especially in the case of lonely people living in the urban environment and NHC (nursing-home care) residents.

Electrical bioimpedance in patients after ischemic stroke, a civilization disease.

INTRODUCTION: Ischemic stroke is caused by artery blockage, resulting in damage to brain tissue. A significant factor in the further treatment of a patient is not only the time to reach a specialist centre, but also the state of hydration of the organism. Clinical situations involving the excess or deficiency of water can have extremely serious consequences for the functioning of the body and subsequent stroke-related disorders. One of the increasingly common methods of evaluating the composition of the human body is by electrical bioimpedance. OBJECTIVE: The aim of the study was to assess the state of human body hydration measured by bioimpedance in patients with an early stage of ischemic stroke. MATERIAL AND METHODS: The examination involved 81 patients with ischemic stroke in the Neurology Department, Subdivision of Stroke Treatment at the Province Specialist Hospital in Lublin, Poland, in 2015. Measurement of the composition and outer and intracellular space of the human body by the Whole Body Bioimpedance (WBIA) method was performed using the Body Composition Monitor (BCM) (Fresenius Medical Care GmbH, Bad Homburg, Germany). RESULTS: A group of patients were been examined with the use of the BIA apparatus three times: on the first, seventh, and tenth day of hospital stay, to determine the level of body hydration. Comparative analysis of data showed that on the first day of hospitalization all the measurements of electrical bioimpedance parameters in the patients were significantly different from those of the control group. In the case of TBW, ECW, ICW, the patients' scores were significantly higher than those of the control group. Only with respect to the over-hydration index, the patients achieved a significantly lower score than the control group. CONCLUSIONS: Assessment of hydration status in patients with a diagnosed stroke indicates a slight dehydration in relation to the control group, but falling within the scope of normovolaemia, according to bioimpedance measurement standards.

Differences in the quality of life dependent on family status of the elderly living in rural areas - a cross-sectional survey.

INTRODUCTION: A factor that conditions the perception of the quality of life (QoL) is having a social network and relationships within it. The quality of life of seniors is assumed to be at a higher level if they are surrounded by those close to them in comparison to those living alone. The aim of the study was to assess differences in the quality of life of elderly rural residents depending on their family status. MATERIAL AND METHODS: Due to the random and mixed selection of respondents, the study comprised a group of 588 representatives living in rural areas of Eastern Poland. The differentiated criterion of the groups of senior respondents was their family situation: living with a family or living alone. Assessment of the quality of life was conducted by means of the WHOQoL-bref questionnaire. The Mann-Whitney tests and Kruskal-Wallis tests were used to carry out statistical analysis of the data. RESULTS: The respondents who lived with their families differed statistically to a significant extent (p<0.005) from those who lived alone. The former gave a better assessment of the majority of the QoL domains: physical, psychological and social. Obtaining higher mean values for the environmental domain among the respondents living alone (M=14.31) seemed to be an atypical and interesting phenomenon because seniors living with their relatives usually assessed that dimension better than those living alone. CONCLUSIONS: The family situation of seniors affected the level of quality of life. Living with their relatives may be expected to be favourable for seniors because it translates into better performance in physical, psychological and social domains. Loneliness, which frequently accompanies old age, leads to the deterioration of the quality of life.

Differences in the Perception of Social Support Among Rural Area Seniors-A Cross-Sectional Survey of Polish Population.

Introduction and objective: Social support constitutes an important determinant of an elderly person’s health and of functioning in his or her living environment. It depends on available support networks and the type of help received. Measurement of social support should encompass both its structure and the functions it fulfills, which enables detailed assessment of the phenomenon. The aim of the study was to compare the perception of social support among rural area seniors provided with institutional care with those living in a home setting. Material and method: Using the diagnostic survey method and the technique of the distribution of a direct questionnaire, 364 respondents from rural areas were examined: those living in an institutional environment (n = 190) and those living in their home (natural) environment (n = 174). The respondents were selected on the basis of a combined sampling method: proportionate, stratified, and systematic. Variables were measured with the following questionnaires: Courage Social Network Index (CSNI) and Social Support Scale (SSS). Results: The living environment has been proved to differentiate average values of support both in the structural and functional dimensions in a statistically significant way (p < 0.001). An untypical phenomenon was higher average values pertaining to emotional bonds, frequency of direct contacts, and help received in the group of respondents living in an institutional environment. Conclusions: The living environment and demographic variables affect the perception of social support among elderly people. Full-time institutional care of a senior citizen leads to the deterioration of social support; therefore, keeping an elderly person in a home environment should be one of the primary goals of the senior policy.