Landscape characteristics shape surface soil microbiomes in the Chihuahuan Desert.

Introduction: Soil microbial communities, including biological soil crust microbiomes, play key roles in water, carbon and nitrogen cycling, biological weathering, and other nutrient releasing processes of desert ecosystems. However, our knowledge of microbial distribution patterns and ecological drivers is still poor, especially so for the Chihuahuan Desert. Methods: This project investigated the effects of trampling disturbance on surface soil microbiomes, explored community composition and structure, and related patterns to abiotic and biotic landscape characteristics within the Chihuahuan Desert biome. Composite soil samples were collected in disturbed and undisturbed areas of 15 long-term ecological research plots in the Jornada Basin, New Mexico. Microbial diversity of cross-domain microbial groups (total Bacteria, Cyanobacteria, Archaea, and Fungi) was obtained via DNA amplicon metabarcode sequencing. Sequence data were related to landscape characteristics including vegetation type, landforms, ecological site and state as well as soil properties including gravel content, soil texture, pH, and electrical conductivity. Results: Filamentous Cyanobacteria dominated the photoautotrophic community while Proteobacteria and Actinobacteria dominated among the heterotrophic bacteria. Thaumarchaeota were the most abundant Archaea and drought adapted taxa in Dothideomycetes and Agaricomycetes were most abundant fungi in the soil surface microbiomes. Apart from richness within Archaea (p = 0.0124), disturbed samples did not differ from undisturbed samples with respect to alpha diversity and community composition (p ≥ 0.05), possibly due to a lack of frequent or impactful disturbance. Vegetation type and landform showed differences in richness of Bacteria, Archaea, and Cyanobacteria but not in Fungi. Richness lacked strong relationships with soil variables. Landscape features including parent material, vegetation type, landform type, and ecological sites and states, exhibited stronger influence on relative abundances and microbial community composition than on alpha diversity, especially for Cyanobacteria and Fungi. Soil texture, moisture, pH, electrical conductivity, lichen cover, and perennial plant biomass correlated strongly with microbial community gradients detected in NMDS ordinations. Discussion: Our study provides first comprehensive insights into the relationships between landscape characteristics, associated soil properties, and cross-domain soil microbiomes in the Chihuahuan Desert. Our findings will inform land management and restoration efforts and aid in the understanding of processes such as desertification and state transitioning, which represent urgent ecological and economical challenges in drylands around the world.

Biocrust carbon exchange varies with crust type and time on Chihuahuan Desert gypsum soils.

Introduction: In dryland systems, biological soil crusts (biocrusts) can occupy large areas of plant interspaces, where they fix carbon following rain. Although distinct biocrust types contain different dominant photoautotrophs, few studies to date have documented carbon exchange over time from various biocrust types. This is especially true for gypsum soils. Our objective was to assess the carbon exchange of biocrust types established at the world's largest gypsum dune field at White Sands National Park. Methods: We sampled five different biocrust types from a sand sheet location in three different years and seasons (summer 2020, fall 2021, and winter 2022) for carbon exchange measurements in controlled lab conditions. Biocrusts were rehydrated to full saturation and light incubated for 30 min, 2, 6, 12, 24, and 36 h. Samples were then subject to a 12-point light regime with a LI-6400XT photosynthesis system to determine carbon exchange. Results: Biocrust carbon exchange values differed by biocrust type, by incubation time since wetting, and by date of field sampling. Lichens and mosses had higher gross and net carbon fixation rates than dark and light cyanobacterial crusts. High respiration rates were found after 0.5 h and 2 h incubation times as communities recovered from desiccation, leveling off after 6 h incubation. Net carbon fixation of all types increased with longer incubation time, primarily as a result of decreasing respiration, which suggests rapid recovery of biocrust photosynthesis across types. However, net carbon fixation rates varied from year to year, likely as a product of time since the last rain event and environmental conditions preceding collection, with moss crusts being most sensitive to environmental stress at our study sites. Discussion: Given the complexity of patterns discovered in our study, it is especially important to consider a multitude of factors when comparing biocrust carbon exchange rates across studies. Understanding the dynamics of biocrust carbon fixation in distinct crust types will enable greater precision of carbon cycling models and improved forecasting of impacts of global climate change on dryland carbon cycling and ecosystem functioning.

Abrupt transitions in a southwest USA desert grassland related to the Pacific Decadal Oscillation.

Prediction of abrupt ecosystem transitions resulting from climatic change will be an essential element of adaptation strategies in the coming decades. In the arid southwest USA, the collapse and recovery of long-lived perennial grasses have important effects on ecosystem services, but the causes of these variations have been poorly understood. Here we use a quality-controlled vegetation monitoring dataset initiated in 1915 to show that grass cover dynamics during the 20th century were closely correlated to the Pacific decadal oscillation (PDO) index. The relationship out-performed models correlating grasses to yearly precipitation and drought indices, suggesting that ecosystem transitions attributed only to local disturbances were instead influenced by climate teleconnections. Shifts in PDO phase over time were associated with the persistent loss of core grass species and recovery of transient species, so recovery of grasses in aggregate concealed significant changes in species composition. However, the relationship between PDO and grass cover broke down after 1995; grass cover is consistently lower than PDO would predict. The decoupling of grass cover from the PDO suggests that a threshold had been crossed in which warming or land degradation overwhelmed the ability of any grass species to recover during favorable periods.

Impact of Different Mechanical and Metabolic Stimuli on the Temporal Dynamics of Muscle Strength Adaptation.

ABSTRACT: Lambrianides, Y, Epro, G, Smith, K, Mileva, K, James, D, and Karamanidis, K. Impact of different mechanical and metabolic stimuli on the temporal dynamics of muscle strength adaptation. J Strength Cond Res 36(11): 3246-3255, 2022-A fundamental task in exercise physiology is to determine and ultimately improve the adaptations that take place in the human body, an integrated network of various physiological systems, for example, muscle, tendon, and bone. Investigating the temporal dynamics (time course) of adaptations in these diverse systems may help us gain new knowledge about the functioning of the neuromotor system in healthy and pathological conditions. The aim of this review was to explore the temporal dynamics of muscular strength adaptations in studies implementing a resistance training intervention. In addition, we categorized these studies under mechanical or metabolic stimuli to identify whether certain stimuli cause faster muscle strength gains. Searches were performed using PubMed and Google Scholar databases. The review comprised 708 subjects from 57 training groups within 40 studies that met the inclusion criteria. The results revealed that the mean time point of first significant increase in muscle strength of all studies was 4.3 weeks, and the corresponding increase was on average about 17%. A plateau in muscle strength increase (∼25%) was found to occur between weeks 8 and 12. Categorization into stimuli groups revealed that performing training in a hypoxic environment is likely to produce a leftward shift (∼25% increase at ∼2.8 weeks) in the dose-response relationship compared with blood flow restriction and supplementation. However, stimuli that cause faster muscle strength gains may also induce imbalanced adaptation between the muscle and the surrounding biological structures, potentially triggering a degradation in some parts of the network (i.e., leading to an increased risk of injury).

Reliability and Accuracy of a Time-Efficient Method for the Assessment of Achilles Tendon Mechanical Properties by Ultrasonography.

The assessment of the force-length relationship under mechanical loading is widely used to evaluate the mechanical properties of tendons and to gain information about their adaptation, function, and injury. This study aimed to provide a time-efficient ultrasound method for assessing Achilles tendon mechanical properties. On two days, eleven healthy young non-active adults performed eight maximal voluntary isometric ankle plantarflexion contractions on a dynamometer with simultaneous ultrasonographic recording. Maximal tendon elongation was assessed by digitizing ultrasound images at rest and at maximal tendon force. Achilles tendon stiffness index was calculated from the ratio of tendon force-to-strain. No within- and between-day differences were detected between the proposed method and manual frame by frame tracking in Achilles tendon maximal force, maximal elongation, maximal strain, and stiffness index. The overall coefficient of variation between trials ranged from 3.4% to 10.3% and average difference in tendon tracking between methods was less than 0.6% strain. Furthermore, an additional assessment demonstrated significant differences between elite athletes, healthy young, and older adults in Achilles tendon force and stiffness index. Hence, the analysis has the potential to reliably and accurately monitor changes in Achilles tendon mechanical properties due to aging and altered mechanical loading in a time-efficient manner.

'The last thing you feel is the self-disgust'. The role of self-directed disgust in men who have attempted suicide: A grounded theory study.

OBJECTIVE: Globally, suicide affects more men than women. Emotional pain underpins many theoretical accounts of suicidality, yet little is known about the role of disgust in suicide. Self-directed disgust, whereby aspects of the self-serve as an object of disgust, has been hypothesised to factor in suicide. This research aimed to explore the processes which link self-disgust to attempted suicide in males. METHOD: Nine men who had attempted suicide completed semi-structured interviews. The interview data were analysed using a constructivist grounded theory methodology. RESULTS: Three concepts emerged out of the analysis: (1) self-disgust; (2) worthlessness; and (3) the endured emotional distress of 'the abyss'" - these concepts interweaved, leading the men to experience hopelessness, disconnection and an inability to cope, leading ultimately to their suicide attempt. Throughout this journey, various disgust-related processes worsened men's distress and increased their suicidal risk. Historic adversities prevailed across the data, as did the men's difficulties in understanding their emotions. CONCLUSION: Self-disgust was an important emotion in the men's experiences of suicide and shaped their views of themselves and their lives. The distancing and repellent properties of self-disgust, in addition to the fear of having their 'disgustingness' exposed, increased suicidal risk. Self-disgust appeared more pervasive in the suicidality of men with a history of multiple childhood adversities. The limitations of this research are discussed as implications for clinical practice and directions for future research.

A voluntary activation deficit in m. abductor hallucis exists in asymptomatic feet.

M. abductor hallucis (AbH) is the strongest intrinsic foot muscle and its dysfunction underlies various foot disorders. Attempts to strengthen the muscle by voluntary exercises are constrained by its complex morphology and oblique mechanical action, which leads to an inability even in asymptomatic individuals to fully activate AbH. This study investigated the extent and magnitude of this inability whilst also providing preliminary evidence for the virtue of targeted sub-maximum neuromuscular electrical stimulation (NMES) as a countermeasure for an AbH activation deficit. The voluntary activation ratio (VAR) was assessed via the twitch interpolation technique in the left AbH of 13 healthy participants during maximum voluntary 1st metatarsophalangeal joint flexion-abduction contractions (MVC). Participants were grouped ("able" or "unable") based on their ability to fully activate AbH (VAR ≥ 0.9). 7 s-NMES trains (20 Hz) were then delivered to AbH with current intensity increasing from 150% to 300% motor threshold (MT) in 25% increments. Perceived comfort was recorded (10 cm-visual analogue scale; VAS). Only 3 participants were able to activate AbH to its full capacity (able, mean (range) VAR: 0.93 (0.91-0.95), n = 3; unable: 0.69 (0.36-0.83), n = 10). However, the maximum absolute forces produced during the graded sub-maximum direct-muscle NMES protocol were comparable between groups implying that the peripheral contractility of AbH is intact irrespective of the inability of individuals to voluntary activate AbH to its full capacity. These findings demonstrate that direct-muscle NMES overcomes the prevailing inability for high voluntary AbH activation and therefore offers the potential to strengthen the healthy foot and restore function in the pathological foot.

Leaning the Trunk Forward Decreases Patellofemoral Joint Loading During Uneven Running.

ABSTRACT: AminiAghdam, S, Epro, G, James, D, and Karamanidis, K. Leaning the trunk forward decreases patellofemoral joint loading during uneven running. J Strength Cond Res 36(12): 3345-3351, 2022-Although decline surfaces or a more upright trunk posture during running increase the patellofemoral joint (PFJ) contact force and stress, less is known about these kinetic parameters under simultaneous changes to the running posture and surface height. This study aimed to investigate the interaction between Step (10-cm drop-step and level step) and Posture (trunk angle from the vertical: self-selected, ∼15°; backward, ∼0°; forward, ∼25°) on PFJ kinetics (primary outcomes) and knee kinematics and kinetics as well as hip and ankle kinetics (secondary outcomes) in 12 runners at 3.5 ms -1 . Two-way repeated measures analyses of variance ( α = 0.05) revealed no step-related changes in peak PFJ kinetics across running postures; however, a decreased peak knee flexion angle and increased joint stiffness in the drop-step only during backward trunk-leaning. The Step main effect revealed significantly increased peak hip and ankle extension moments in the drop-step, signifying pronounced mechanical demands on these joints. The Posture main effect revealed significantly higher and lower PFJ kinetics during backward and forward trunk-leaning, respectively, when compared with the self-selected condition. Forward trunk-leaning yielded significantly lower peak knee extension moments and higher hip extension moments, whereas the opposite effects occurred with backward trunk-leaning. Overall, changes to the running posture, but not to the running surface height, influenced the PFJ kinetics. In line with the previously reported efficacy of forward trunk-leaning in mitigating PFJ stress while even or decline running, this technique, through a distal-to-proximal joint load redistribution, also seems effective during running on surfaces with height perturbations.

Interjoint Coordination in Kicking a Moving Target: A Comparison Between Elite and Nonelite Taekwondo Players.

Patterns of interjoint coordination in the kicking legs of taekwondo players were investigated to understand movement pattern variability as a functional property of skill level. Elite and nonelite players performed roundhouse kicks against a custom-built moving target fitted with an accelerometer, and movements were recorded by motion capture. Average foot segment velocities of 13.6 and 11.4 m/s were recorded for elite and nonelite players, respectively (P < .05), corresponding to target accelerations of 87.5 and 70.8g (P < .05). Gradient values derived from piecewise linear regression of continuous relative phase curves established the comparative incoordination of nonelite taekwondo players in the form of an overshoot behavior during the crucial period leading to target impact (P < .05). This overshoot was apparent in both knee-hip and ankle-knee continuous relative phase curves. Elite players generated greater limb speed and impact force through more effective limb segment coordination. The combination of continuous relative phase and piecewise linear regression techniques allowed identification of alternate joint control approaches in the 2 groups.

Quadrat-based monitoring of desert grassland vegetation at the Jornada Experimental Range, New Mexico, 1915-2016.

The data set covers a 101-yr period (1915-2016) of quadrat-based plant sampling at the Jornada Experimental Range in southern New Mexico. At each sampling event, a pantograph was used to record the location and perimeter of living plants within permanent quadrats. Basal area was recorded for perennial grass species, canopy cover area was recorded for shrub species, and all other perennial species were recorded as point data. The data set includes 122 1 × 1 m permanent quadrats, although not all quadrats were sampled in each year of the study and there is a gap in monitoring from 1980 to 1995. These data provide a unique opportunity to investigate changes in the plant community over 100 yr of variation in precipitation and other environmental conditions. We provide the following data and data formats: (1) the digitized maps in shapefile format; (2) a data table containing coordinates (x, y) of perennial species within quadrats, including cover area for grasses and shrubs; (3) a data table of counts of annual plant individuals per quadrat; (4) a species list indicating growth form and habit of recorded species; (5) a table of dates when each quadrat was sampled; (6) a table of the pasture each quadrat was located within (note that pasture boundaries have changed over time); (7) a table of depth to petrocalcic layer measurements taken at quadrat locations; (8) a table of particle size analysis of soil samples taken at quadrat locations; (9) a table of topographic characteristics of quadrat locations (e.g., concave or convex topography). Pantograph sampling is currently conducted at 5-yr intervals by USDA-ARS staff, and new data will be added periodically to the EDI Data Portal Repository (see section V.E.2). This information is released under the Creative Commons license-Attribution-CC BY and the consumer of these data is required to cite it appropriately in any publication that results from its use.

Identifying consistent biomechanical parameters across rising-to-walk subtasks to inform rehabilitation in practice: A systematic literature review.

BACKGROUND: The best approach to rehabilitate the control of everyday whole-body movement (e.g. rise-to-walk) after pathology remains unclear in part because the associated controlled performance variables are not known. Rise-to-walk can be performed fluidly (sit-to-walk) or non-fluidly (sit-to-stand, proceeded by gait-initiation). Biomechanical variables that remain consistent in health regardless of how rise-to walk is performed represent controlled performance variable candidates which could monitor rehabilitative change. RESEARCH QUESTION: To determine if any biomechanical parameters remain consistent across rising-to-walk (RTW) subtasks (sit-to-stand, gait-initiation, and sit-to-walk) in healthy adults for purposes of movement control assessment in clinical practice. METHODS: Data sources included Medline, Cinahl, and Scopus databases, and the grey literature. Study selection was based on eligibility criteria and must have reported spatiotemporal, kinematic and/or kinetic biomechanical parameters featuring >1 RTW subtask. Data extraction and synthesis; standardised-mean-differences (SMDs) were calculated (pooled if replicated in >1 study) for each parameter. Consistency was determined if SMD95 %CIs included the zero-effect line. RESULTS: Nine studies (n = 99) were included (40 ±â€¯7.5yrs). Seven parameters were replicated in >1 study and subjected to meta-analysis (fixed-effect model). Two were consistent between sit-to-stand and sit-to-walk: flexion-momentum time (M(95 %CI) = 0.055(-0.423 to 0.533); p = 0.823) and peak whole-body-centre-of-mass vertical velocity (M(95 %CI)= -0.415(-0.898 to 0.069); p = 0.093); and centre-of-pressure to whole-body-centre-of-mass distance at toe-off (M(95 %CI)= -0.137(-0.712 to 0.439); p = 0.642) between gait-initiation and sit-to-walk. Another 20 parameters were consistent based on single-study SMDs. SIGNIFICANCE: Consistent parameters might exist across RTW subtasks. However, the evidence is based on few studies with small samples and variable RTW protocols. Future studies designed to confirm consistency using a standardised RTW protocol are needed.

Direct muscle electrical stimulation as a method for the in vivo assessment of force production in m. abductor hallucis.

In vivo assessment of the force-generating capacity of m. abductor hallucis (AbH) is problematic due to its combined abduction-flexion action and the inability of some individuals to voluntarily activate the muscle. This study investigated direct muscle electrical stimulation as a method to assess isometric force production in AbH about the 1st metatarsal phalangeal joint (1MPJ) at different muscle-tendon lengths, with the aim of identifying an optimal angle for force production. A 7 s stimulation train was delivered at 20 Hz pulse frequency and sub-maximal (150% motor threshold) intensity to the AbH of the left foot in 16 participants whilst seated, and with the Hallux suspended from a force transducer in 0°,5°,10°,15° and 20° 1MPJ dorsal flexion. Reflective markers positioned on the foot and force transducer were tracked with 5 optical cameras to continuously record the force profile and calculate the external 1MPJ joint flexion moment at each joint configuration. A parabolic relationship was found between AbH force production and 1MPJ configuration. The highest 1MPJ joint moments induced by electrical stimulation were found between 10° and 15° of Hallux dorsal flexion. However, the joint angle (p < 0.001; η2 = 0.86) changed significantly across all but one 1MPJ configurations tested during the stimulation-evoked contraction, resulting in a significant change in the corresponding external moment arm (p < 0.001; η2 = 0.83). Therefore, the changes in joint geometry during contraction should be accounted for to prevent an underestimation of the resulting joint moment. We conclude that direct muscle electrical stimulation combined with dynamometry offers a robust method for standardised assessment of AbH sub-maximal isometric force production.

Gait-initiation onset estimation during sit-to-walk: Recommended methods suitable for healthy individuals and ambulatory community-dwelling stroke survivors.

BACKGROUND: Gait-initiation onset (GI-onset) during sit-to-walk (STW) is commonly defined by mediolateral ground-reaction-force (xGRF) rising and crossing a threshold pre-determined from sit-to-stand peak xGRF. However, after stroke this method [xGRFthresh] lacks validity due to impaired STW performance. Instead, methodologies based upon instance of swing-limb maximum-vertical-GRF [vGRFmaxSWING], maximum-xGRF [xGRFmax], and swing-limb heel-off [firstHEELoff] can be applied, although their validity is unclear. Therefore, we determined these methodologies' validity by revealing the shortest transition-time (seat-off-GI-onset), their utility in routinely estimating GI-onset, and whether they exhibited satisfactory intra-subject reliability. METHODS: Twenty community-dwelling stroke (60 (SD 14) years), and twenty-one age-matched healthy volunteers (63 (13) years) performed 5 standardised STW trials with 2 force-plates and optical motion-tracking. Transition-time differences across-methods were assessed using Friedman tests with post-hoc pairwise-comparisons. Within-method single-measure intra-subject reliability was determined using ICC3,1 and standard errors of measurement (SEMs). RESULTS: In the healthy group, median xGRFthresh transition-time was significantly shorter than xGRFmax (0.183s). In both the healthy and stroke groups, xGRFthresh transition-times (0.027s, 0.695s respectively) and vGRFmaxSWING (0.080s, 0.522s) were significantly shorter than firstHEELoff (0.293s, 1.085s) (p<0.001 in all cases). GI-onset failed to be estimated in 48% of stroke trials using xGRFthresh. Intra-subject variability was relatively high but was comparable across all estimation methods. CONCLUSION: The firstHEELoff method yielded significantly longer transition-times. The xGRFthresh method failed to routinely produce an estimation of GI-onset estimation. Thus, with all methods exhibiting low, yet comparable intra-subject repeatability, averaged xGRFmax or vGRFmaxSWING repeated-measures are recommended to estimate GI-onset for both healthy and community-dwelling stroke individuals.

Lower Body Acceleration and Muscular Responses to Rotational and Vertical Whole-Body Vibration at Different Frequencies and Amplitudes.

AIM: The aim of this study was to characterize acceleration transmission and neuromuscular responses to rotational vibration (RV) and vertical vibration (VV) at different frequencies and amplitudes. METHODS: Twelve healthy males completed 2 experimental trials (RV vs VV) during which vibration was delivered during either squatting (30°; RV vs VV) or standing (RV only) with 20, 25, and 30 Hz, at 1.5 and 3.0 mm peak-to-peak amplitude. Vibration-induced accelerations were assessed with triaxial accelerometers mounted on the platform and bony landmarks at ankle, knee, and lumbar spine. RESULTS: At all frequency/amplitude combinations, accelerations at the ankle were greater during RV (all P < .03) with the greatest difference observed at 30 Hz, 1.5 mm. Transmission of RV was also influenced by body posture (standing vs squatting, P < .03). Irrespective of vibration type, vibration transmission to all skeletal sites was generally greater at higher amplitudes but not at higher frequencies, especially above the ankle joint. Acceleration at the lumbar spine increased with greater vibration amplitude but not frequency and was highest with RV during standing. CONCLUSIONS/IMPLICATIONS: The transmission of vibration during whole-body vibration (WBV) is dependent on intensity and direction of vibration as well as body posture. For targeted mechanical loading at the lumbar spine, RV of higher amplitude and lower frequency vibration while standing is recommended. These results will assist with the prescription of WBV to achieve desired levels of mechanical loading at specific sites in the human body.

Parameters that remain consistent independent of pausing before gait-initiation during normal rise-to-walk behaviour delineated by sit-to-walk and sit-to-stand-and-walk.

BACKGROUND: Rising-to-walk is an everyday transitional movement task rarely employed in gait rehabilitation. Sit-to-walk (STW) and sit-to-stand-and-walk (STSW), where a pause separates sit-to-stand and gait-initiation (GI) represent extremes of rising-to-walk behaviour. Delayed GI can indicate pathological impairment but is also observed in healthy individuals. We hypothesise that healthy subjects express consistent biomechanical parameters, among others that differ, during successful rising-to-walk task performance regardless of behaviour. This study therefore sought to identify if any parameters are consistent between STW and STSW in health because they represent normal rise-to-walk performance independent of pause, and also because they represent candidate parameters sensitive enough to monitor change in pathology. METHODS: Ten healthy volunteers performed 5 trials of STW and STSW. Event timing, ground-reaction-forces (GRFs), whole-body-centre-of-mass (BCoM) displacement, and centre-of-pressure (CoP) to extrapolated BCoM (xCoM) distance (indicator of positional stability) up to the 3rd step were compared between-tasks with paired t-tests. For consistent parameters; agreement between-tasks was assessed using Bland-Altman analyses and minimal-detectable-change (MDC) calculations. RESULTS: Mean vertical GRFs, peak forward momentum and fluidity during rising; CoP-xCoM separation at seat-off, upright, GI-onset, and steps1-2; and forward BCoM velocity were all significantly greater in STW. In contrast, peak BCoM vertical momentum, flexion-momentum time, and 3rd step stability were consistent between tasks and yielded acceptable reliability. CONCLUSION: STW is a more challenging task due to the merging of rising with GI reflected by greater CoP-xCoM separation compared to STSW indicative of more positional instability. However, BCoM vertical momentum, flexion-momentum time, and step3 stability remained consistent in healthy individuals and are therefore candidates with which to monitor change in gait rehabilitation following pathology. Future studies should impose typical pause-durations observed in pathology upon healthy subjects to determine if the parameters we have identified remain consistent.

Wide-pulse, high-frequency, low-intensity neuromuscular electrical stimulation has potential for targeted strengthening of an intrinsic foot muscle: a feasibility study.

BACKGROUND: Strengthening the intrinsic foot muscles is a poorly understood and largely overlooked area. In this study, we explore the feasibility of strengthening m. abductor hallucis (AH) with a specific paradigm of neuromuscular electrical stimulation; one which is low-intensity in nature and designed to interleave physiologically-relevant low frequency stimulation with high-frequencies to enhance effective current delivery to spinal motoneurones, and enable a proportion of force produced by the target muscle to be generated from a central origin. We use standard neurophysiological measurements to evaluate the acute (~ 30 min) peripheral and central adaptations in healthy individuals. METHODS: The AH in the dominant foot of nine healthy participants was stimulated with 24 × 15 s trains of square wave (1 ms), constant current (150% of motor threshold), alternating (20 Hz-100 Hz) neuromuscular electrical stimulation interspersed with 45 s rest. Prior to the intervention, peripheral variables were evoked from the AH compound muscle action potential (Mwave) and corresponding twitch force in response to supramaximal (130%) medial plantar nerve stimulation. Central variables were evoked from the motor evoked potential (MEP) in response to suprathreshold (150%) transcranial magnetic stimulation of the motor cortex corresponding to the AH pathway. Follow-up testing occurred immediately, and 30 min after the intervention. In addition, the force-time-integrals (FTI) from the 1st and 24th WPHF trains were analysed as an index of muscle fatigue. All variables except FTI (T-test) were entered for statistical analysis using a single factor repeated measures ANOVA with alpha set at 0.05. RESULTS: FTI was significantly lower at the end of the electrical intervention compared to that evoked by the first train (p < 0.01). Only significant peripheral nervous system adaptations were observed, consistent with the onset of low-frequency fatigue in the muscle. In most of these variables, the effects persisted for 30 min after the intervention. CONCLUSIONS: An acute session of wide-pulse, high-frequency, low-intensity electrical stimulation delivered directly to abductor hallucis in healthy feet induces muscle fatigue via adaptations at the peripheral level of the neuromuscular system. Our findings would appear to represent the first step in muscle adaptation to training; therefore, there is potential for using WPHF for intrinsic foot muscle strengthening.

Loading rate and contraction duration effects on in vivo human Achilles tendon mechanical properties.

Tendons are viscoelastic, which implies loading rate dependency, but loading rates of contractions are often not controlled during assessment of human tendon mechanical properties in vivo. We investigated the effects of sustained submaximal isometric plantarflexion contractions, which potentially negate loading rate dependency, on the stiffness of the human Achilles tendon in vivo using dynamometry and ultrasonography. Maximum voluntary contractions (high loading rate), ramp maximum force contractions with 3 s loading (lower loading rate) and sustained contractions (held for 3 s) at 25%, 50% and 80% of maximal tendon force were conducted. No loading rate effect on stiffness (25-80% max. tendon force) was found. However, loading rate effects were seen up to 25% of maximum tendon force, which were reduced by the sustained method. Sustained plantarflexion contractions may negate loading rate effects on tendon mechanical properties and appear suitable for assessing human Achilles tendon stiffness in vivo.

Sit-to-stand-and-walk from 120% Knee Height: A Novel Approach to Assess Dynamic Postural Control Independent of Lead-limb.

Individuals with sensorimotor pathology e.g., stroke have difficulty executing the common task of rising from sitting and initiating gait (sit-to-walk: STW). Thus, in clinical rehabilitation separation of sit-to-stand and gait initiation - termed sit-to-stand-and-walk (STSW) - is usual. However, a standardized STSW protocol with a clearly defined analytical approach suitable for pathological assessment has yet to be defined. Hence, a goal-orientated protocol is defined that is suitable for healthy and compromised individuals by requiring the rising phase to be initiated from 120% knee height with a wide base of support independent of lead limb. Optical capture of three-dimensional (3D) segmental movement trajectories, and force platforms to yield two-dimensional (2D) center-of-pressure (COP) trajectories permit tracking of the horizontal distance between COP and whole-body-center-of-mass (BCOM), the decrease of which increases positional stability but is proposed to represent poor dynamic postural control. BCOM-COP distance is expressed with and without normalization to subjects' leg length. Whilst COP-BCOM distances vary through STSW, normalized data at the key movement events of seat-off and initial toe-off (TO1) during steps 1 and 2 have low intra and inter subject variability in 5 repeated trials performed by 10 young healthy individuals. Thus, comparing COP-BCOM distance at key events during performance of an STSW paradigm between patients with upper motor neuron injury, or other compromised patient groups, and normative data in young healthy individuals is a novel methodology for evaluation of dynamic postural stability.

Sit-to-walk and sit-to-stand-and-walk task dynamics are maintained during rising at an elevated seat-height independent of lead-limb in healthy individuals.

INTRODUCTION: Sit-to-walk (STW) is a common transitional motor task not usually included in rehabilitation. Typically, sit-to-stand (STS), pause, then gait initiation (GI) before walking is used, which we term sit-to-stand-and-walk (STSW). Separation between centre-of-pressure (COP) and whole-body centre-of-mass (BCOM) during GI is associated with dynamic postural stability. Rising from seats higher than knee-height (KH) is more achievable for patients, but whether this and/or lead-limb significantly affects task dynamics is unclear. This study tested whether rising from seat-heights and lead-limb affects STW and STSW task dynamics in young healthy individuals. METHODS: Ten (5F) young (29±7.7 years) participants performed STW and STSW from a standardised position. Five trials of each task were completed at 100 and 120%KH leading with dominant and non-dominant legs. Four force-plates and optical motion capture delineated key movement events and phases with effect of seat-height and lead-limb determined by 2-way ANOVA within tasks. RESULTS: At 120%KH, lower peak vertical ground-reaction-forces (vGRFs) and vertical BCOM velocities were observed during rising irrespective of lead-limb. No other parameters differed between seat-heights or lead-limbs. During GI in STSW there was more lateral, and less posterior, COP excursion than expected. CONCLUSION: Reduction in vGRFs and velocity during rising at 120%KH is consistent with reduced effort in young healthy individuals and is likely therefore to be an appropriate seat-height for patients. Lead-limb had no effect upon STSW or STW parameters suggesting that normative data independent of lead-limb can be utilised to monitor motor rehabilitation should differences be observed in patients. STSW should be considered an independent movement transition.

Experiences of using pro-eating disorder websites: a qualitative study with service users in NHS eating disorder services.

PURPOSE: Previous research into the impact of pro-eating disorder (pro-ED) websites has predominantly been undertaken using experimental and survey designs. Studies have used both clinical and non-clinical (college student) samples. The present study aimed to explore the underlying functions and processes related to the access and continued use of pro-ED websites within a clinical eating disorder population using a qualitative research design. METHODS: Participants were recruited through NHS community mental health teams and specialist eating disorder services within South Wales, UK. Face-to-face semi-structured interviews were conducted with seven adult women in treatment for an eating disorder who had disclosed current or historic use of pro-ED websites. Interviewees ranged in age from 20 to 40 years (M = 31.2; SD = 7.8). Constructivist Grounded Theory was used to analyse interview transcripts. RESULTS: Five key themes were identified within the data, namely fear; ambivalence; social comparisons; shame; and pro-ED websites maintaining eating disordered behaviour. The pro-ED websites appeared to offer a sense of support, validation and reassurance to those in the midst of an eating disorder, whilst simultaneously reinforcing and maintaining eating disordered behaviour. CONCLUSION: Themes are discussed in relation to implications and recommendations for clinical practice. Limitations of the present study and suggestions for future research are also outlined.