RESUMO
The Aspergillus galactomannan enzyme-linked immunosorbant assay (EIA) has been demonstrated to facilitate rapid and sensitive detection of invasive aspergillosis. However, test specificity has not been fully evaluated in non-Aspergillus fungal species. Of 53 fungal isolates, cross-reactivity was observed with 5 non-Aspergillus spp.: Blastomyces dermatitidis, Nigrospora oryzae, Paecilomyces lilacinus, Penicillium chrysogenum, and Trichothecium roseum.
Assuntos
Aspergillus/classificação , Mananas/classificação , Técnicas de Tipagem Micológica/métodos , Micoses/diagnóstico , Aspergilose/diagnóstico , Reações Cruzadas , Galactose/análogos & derivados , Humanos , Técnicas Imunoenzimáticas/métodos , Sensibilidade e EspecificidadeRESUMO
We describe a filtration-based culture method to enhance sensitivity of routine culture for recovery of fungi from respiratory specimens. The new method resulted in an 8.3% (P = 0.0039) increase in recovery for all organisms and a 6.4% (P = 0.0391) for Candida species when compared to the conventional culture technique.
