Mercury in the feathers of Golden eagle (Aquila chrysaetos) from Western Carpathian, Slovakia.

In this study, mercury (Hg) concentrations were detected in feathers of golden eagle (Aquila chrysaetos), a bird that typically inhabits alpine and forest areas. The mercury rates in feathers were compared in two groups of eagles: first, estimated home range (breeding, hunting, etc.) was located only in forest and lowland meadow habitats; second, the home range also included alpine habitats-rocks and meadows. Consequently, mercury concentration based on the feather typology were observed and the mercury levels in feathers were also compared among different Slovak Western Carpathian districts. It was found that there was no significant difference between groups classified by elevation level, which we attribute to the fact that eagle hunting territories are broad, so that alpine-dwelling and forest-dwelling eagles do not only reflect the pollution of the environments they typically inhabit. Non-significant differences were found also within different feather types, which means that the type of feather is not crucial for tracking mercury in eagle feathers. As the measurement of feather appears to be a simple and non-invasive method, the detection of non-significant differences in diverse types of golden eagle feathers provides useful knowledge for the future environment monitoring. The average mercury concentration measured in eagle samples was lower than the mercury concentration causing health complications among birds of prey. Our assumption that due to past mining activity in the Spis region, the highest concentration in this region would be observed was confirmed.

Differential accumulation of metals in the lacustrine and fluvial Alpine bullheads (Cottus poecilopus) and recovery of fish from metal contamination after a flash flood.

The spatiotemporal distribution and transport of mercury, zinc, molybdenum, rubidium, and strontium from alpine terrestrial ecosystems to alpine lake and mountain stream populations of Cottus poecilopus were investigated. Metals were measured for 66 wild fish collected from different lakes and Javorinka stream across. Mercury was measured in the pectoral fins, other elements in the skull. Bullheads contained more metals in the alpine lakes than in the mountain stream. In particular, mercury and zinc concentrations in lake bullheads were 6 and 2.5 times higher, respectively, than those of stream-dwelling fish. New data were generated on metal bioaccumulation in fish of understudied West Carpathian alpine lake environments. In July 2018, a major flood occurred in the area of the Javorinka. Already then, the mercury content in bullheads increased significantly. Bioaccumulation of mercury in fish occurred very quickly after the flood and was also significant in the following 2019. Then, the concentrations of mercury quickly decreased up to 70% in 2021-2022. Average concentrations of molybdenum and rubidium in bullheads in the stream rapidly declined in the year following the flood disturbance, but within less than 2 years, the metal levels stabilized at about the same level as in 2017 prior the flood. Strontium concentrations in fish dropped rapidly immediately after the flood, increased in the following years, and dropped again after 4 years, suggesting that many more factors are influencing strontium bioaccumulation in fish that are comparable in magnitude to the flood. The most serious warning seems to be the absence of biogenic zinc. The average concentration in the Alpine bullheads population in the stream has declined by 70% in less than 5 years and is steadily declining. An important result of this study is the demonstration that disturbance by a single factor (heavy rainfall and flooding) has a clear and timely effect on average metal concentrations in the fish population.

Different accumulation of some elements in the fry and adults of alpine bullheads (Cottus poecilopus).

Skulls of alpine bullhead sampled from the Javorinka stream in the Tatra Mountains, West Carpathians, were analyzed to determine concentrations of S, Cl, K, Ca, P, Rb, Zn, Mn, Mb, Fe, Ti, Sn, Co, Ni, Cu, As, Se, Pb, Sb, Ba, Hg, Cr, Ag, and Cd. The stage of development is the most influential factor determining element concentrations in the sampled bullhead, as fry were more polluted than adult fish. The different diets consumed by fry and adult bullhead plays a key role in the accumulation of chemical elements in their bodies. Young bullheads live in small natural embankments containing higher levels of a mixture of sedimentary minerals and microorganisms than in running water. Thus, newly hatched bullheads may serve as excellent indicators of water quality in mountain creeks or streams, as they can indicate the higher pollution of water or prey in their habitats (small bays with sandy bottoms) when compared to the preferred habitat of adult individuals.

Pollution of Feral Pigeon (Columba livia) Depends on Their Age and Their Health Status.

Biomonitoring of synanthropic species provides evidence about effects of the pollution in human environment. In the present study, tibia and tarsometatarsal bones were extracted from feral pigeons (Columba livia), found either deceased, or experimentally captured in the lofts of houses in Bratislava, Slovakia. Concentrations of mercury (tarsometatarsus), lead, iron, and zinc (tibia) were analyzed, along with sex and plumage pattern, wing, and tarsometatarsal length. In order to estimate age, lines of arrested growth (LAGs) were used. Results show no significant differences in heavy metal accumulation depending on sex or plumage pattern. However, age-related tarsometatarsus length was correlated to Hg, Pb, and Fe bone level accumulation. Thus, bigger or older pigeons with longer tarsometatarsal bones had higher Hg, Pb, and Fe concentrations. Higher heavy metal concentrations (mainly Fe and Zn) were also present in bones of older deceased individuals with completed LAG. These findings point to chronic accumulation of heavy metals in feral pigeons during their life in polluted environments.

Temporal and seasonal changes in mercury accumulation in Tatra chamois from West Carpathians.

The main aim of this study was to determine the concentration of total mercury (T-Hg) in different tissues, hair, and faeces from a long-lived animal that actively lives in the subalpine and alpine zone within temperate climate zone throughout the year. Levels of T-Hg in samples from naturally deceased Tatra chamois (n = 72) from the Tatra Mountains (Slovakia) were determined using direct mercury analyses on the basis of dry weight. The mercury concentrations in hair samples were compared over the last three decades. Seasonal changes in mercury concentration and differences in the amounts of mercury in various tissues, hairs, and faeces between age and sex groups were also studied. The highest mercury concentrations in organs were found in the kidneys of Tatra chamois, with mean values of 0.45 ± 0.07 µg/g (dry weight) for adults (n = 18) and 0.39 ± 0.12 µg/g (dry weight) for juveniles (n = 6). These values are considerably high compared to expected Hg levels in wild ungulates from foothill and mountain areas in the temperate zone. Juveniles had higher levels of T-Hg in their muscles (0.011 ± 0.001 µg/g dry weight, n = 10) and faeces (0.189 ± 0.025 µg/g dry weight, n = 7) when compared to adults that had 0.007 ± 0.001 µg/g dry weight of T-Hg in muscles (n = 29) and 0.113 ± 0.015 µg/g dry of T-Hg weight in faeces (n = 19). T-Hg concentrations in individual tissues (heart, kidney, liver, lungs, spleen, tongue, muscle, bone), hairs, and faeces were not significantly different between males and females. Mercury levels in the hair of Tatra chamois have increased significantly since the 1990s (median value of T-Hg: 0.025 µg/g dry weight) with the highest values presenting during the 2000s (0.029 µg/g dry weight). Since 2010 (0.016 µg/g dry weight), levels have declined when compared to those observed during the 1990s and 2000s. Therefore, we can conclude that T-Hg deposition in alpine areas of the Tatra Mountains shows a declining trend since the 2010s. T-Hg concentrations in the heart, muscle, and hair were higher in summer compared to winter. Seasonal changes in mercury concentrations are likely most related to the seasonal availability of food, but may also be related to moulting periods, and this correlation must be explored further.

Ecotoxicology of alpine streams in the West Carpathians-Alpine Bullhead (Cottus poecilopus) and high mountain flash flood effects.

Flash floods represent a serious threat to wildlife, water biota, and human life in pre-alpine regions, particularly in recent historical memory. The alpine bullhead is an established bioindicator of water quality in mountain streams, which can be adversely affected by an increased propensity for flash flooding. The aim of this study was to examine the effect of flash flooding on the variation of chemical elements found in the skull of alpine bullheads, with a focus on inter-annual effects. Their bone tissue reflects an increased concentration of K and Cl and a decreased concentration of biogenic Cr, Zn, and Mo, absorbed mainly through the gills, for up to 2 years following a flood. During autumn, following a summer flash flood, the amount of Mn and Fe present in skull tissues of fish was found to have increased, tapering off again over the following year. These metals are predominantly ingested by bullheads while feeding. The lack of specific types of biogenic concentration in the water may be critical to the definition of presence/absence patterns, as populations were shown to decline 2 to 3 years post-flood. Pre-alpine streams are particularly susceptible to this type of flooding. The decreases in biogenic elements and increase of K and Cl exhibited in bullhead tissues indicate that a negative ecological footprint due to flash floods can still be observed several years following the event.

Occurrence and vertical distribution of Ca, Cl, Cr, Fe, Mn, Mo, K, Rb, Sr, S, Sn, and Zn in the skull bones of alpine bullhead (Cottus poecilopus) in the West Carpathians.

The aim of this study was to examine the contents of calcium, chlorine, chromium, iron, manganese, molybdenum, potassium, rubidium, strontium, sulfur, tin, and zinc in the bones of the alpine bullhead (Cottus poecilopus) related to its vertical distribution in the mountain river Javorinka. This river is located on the northern side of the High Tatras, the West Carpathians. Sampling took place during 2017, 2018, and 2019. One hundred three individuals of bullheads were collected. Only deceased individuals were selected for sampling; there was no deliberate harm to any sampled individuals. Weight, body length, and head length and width were measured. The amounts of Ca, Rb, and Mo were significantly dependent on the altitude and the fish size. At higher altitudes, there are smaller fish that colonize more inaccessible waters with smaller habitat flow options and these smaller fish contain relatively more Ca, Rb, and Mo in their skulls than large fish. The presence of Mn, Sr, and Zn was related to the altitude. Concentrations of Zn and Sr increase with altitude in the alpine bullhead skulls. The amounts of S, Cl, K, Cr, Sn, and Mo did not differ among fish living at different elevations.

Mercury contamination of the snow voles (Chionomys nivalis) in the West Carpathians.

The subject of this research is mercury pollution in the alpine zone of the High Tatra Mountains (the West Carpathians). The snow vole (Chionomys nivalis) was chosen as a bioindicator. Mercury concentrations in the tail tips from captured individuals were examined. The samples were collected over the span of 2 years, from May 2016 to November 2017. The smaller (1-year-old) individuals had higher mercury concentrations in their tails than the bigger (2-year-old) individuals, suggesting recent aerial contamination of alpine habitats. The amount of mercury found in the tails did not differ between sexes. The differences in mercury concentration between animals caught in the spring and autumn were also insignificant. The possible relationship between mercury in the tissues and the seasonal variability of the water cycle in the mountains (snow melting, precipitation) is discussed.

Alpine accentors as monitors of atmospheric long-range lead and mercury pollution in alpine environments.

Mercury and lead are deposited in the West Carpathians as long-range transported air pollution. The Alpine accentor (Prunella collaris) was recognized as a cost-effective biomonitor, and used to investigate the bioavailability of contaminants in large alpine areas. The outer tail feathers and blood of the alpine accentors were used for assessment of atmospheric mercury and lead contamination, respectively. Mean mercury levels in feathers of accentors averaged at 1.15 µg/g (SE = 0.105, n = 40). There were no temporal variations in mercury concentrations. Mean blood lead levels were at 5.2 µg/dL (SE = 0.5, n = 27), showing a slight decreasing trend from July to October. Juveniles were not more susceptible to lead accumulation than adults. Bone lead concentrations that increase with age reflect a bioaccumulation effect. A statistically significant negative correlation was found between the length of erythrocytes and the concentration of lead, which may show the first symptoms of microcytosis. In comparison to aquatic ecosystems, the biogeochemical factors that influence methylmercury availability in alpine habitats are not yet completely known and require further investigation. Our findings show that birds in alpine terrestrial ecosystems may contain surprisingly high levels of methylmercury. The mercury levels in the feathers of accentors probably indicate that alpine autotrophs make sufficient amounts of mercury available to the terrestrial food web. The blood lead levels of accentors likely approach the threshold level for further hematological effects. We found a clear tendency in erythrocytes to change their shape from ellipsoid to smaller and rounder with increasing amounts of lead in their blood. The shape of bird erythrocytes appears to be a very sensitive indicator of critical levels of lead in the alpine environment.

Yersinia species in the dunnock (Prunella modularis) in sub-alpine habitats of the Western Carpathians.

The study presents the prevalence of Yersinia species in dunnok Prunella modularis from the sub-alpine zone of the Western Carpathians. Bacteria were detected from cloacal and pharyngeal swabs from 97 specimens using PCR assay. Yersinia enterocolitica showed the highest prevalence (47.4%) from among the determined Yersinia species. Yersinia species (except Y frederiksenii) were detected more frequently in pharyngeal than cloacal samples. The highest prevalence of yersiniosis was detected in April (Yersinia spp. - 80%, Y. enterocolitica - 70%). No statistically differences were observed in the prevalence of Yersinia spp. between males and females and between juveniles and adult birds. Bacterial contamination did not affect body weight or tarsus length.

The genetic impact of translocations and habitat fragmentation in chamois (Rupicapra) spp.

The chamois is a useful species with which to investigate the combined genetic impact of habitat fragmentation, over hunting, and translocations. Genetic variation within and between chamois (genus Rupicapra) populations was analyzed in 259 individuals from 16 sampling sites located in Italy, Spain, Slovakia, and the Czech Republic. Two mitochondrial DNA markers (control region and cytochrome b) and 11 nuclear microsatellites were typed. The principal results of this study can be summarized as follows: 1) high and significant differentiation between almost all chamois populations is observed even on a microgeographical scale, probably caused by the patchy distribution of this species, sharp geographical barriers to gene flow, and drift effects related to recent bottlenecks; 2) historical translocation events have left a clear genetic signature, including interspecific hybridization in some Alpine localities; 3) the Apennine subspecies of chamois, Rupicapra pyrenaica ornata, shows a high and similar level of divergence (about 1.5 My) from the Pyrenean (Rupicapra pyrenaica pyrenaica) and the Alpine (Rupicapra rupicapra) chamois; therefore, the specific status of these taxa should be revised. These results confirm the potential of population genetic analyses to dissect and interpret complex patterns of diversity in order to define factors important to conservation and management.