Glycopeptide vaccine on DNA-histone carrier and its impact on Z-potential of effector cells during experimental treatment of lymphoblastic leukemia.

AIM: To increase the effectiveness of anticancer vaccine delivery and activity via its immobilization on reconstructed chromatin (RC). MATERIALS AND METHODS: The hybrid mice of CDF-1 line with transplanted P-388 leukemia were used. Reconstructed chromatin was obtained from the thymus of inbred rats. Glycopeptide cancer vaccine prepared from P-388 leukemia cells, was immobilized of RC. ζ-potential of thymocytes from tumor-bearing mice was calculated from Smoluchowski equation. RESULTS: The data have demonstrated the principal possibility of using the fragments of reconstructed chromatin of heterologous origin as a carrier of glycopeptide cancer vaccine prepared from the cells of P-388 leukemia. Preventive immunization with the correspondingly immobilized vaccine normalized ζ-potential of thymocytes in animals with transplanted P­388 leukemia. CONCLUSIONS: The use of immobilization of glycopeptide cancer vaccine on reconstructed chromatin improves anticancer activity of the vaccination.