[Evaluation of anti-mycobacterial antibody IgG concentration in rapid diagnosis of tuberculosis]. / Przydatnosc oceny stezenia przeciwcial przeciwpratkowych klasy IgG w szybkiej diagnostyce gruzlicy pluc.

Tuberculosis is not always easy to diagnose. All the methods applied in diagnosis of this illness have certain limitations. In this study the value of Rapid Test TB in diagnosis of tuberculosis was evaluated. The aim of the test is to detect antibody against mycobacterial antigen 38 kDa. Material consisted of 100 persons divided into 4 groups: BK(+) tuberculosis patients, BK (0) tuberculosis patients--culture (+), lung cancer patients and patients with sarcoidosis. Positive results were obtained in 100% of culture positive patients and in 10% of patients culture (+), in 20% of lung cancer patients and in 40% of patients with sarcoidosis. Specifity of the test was 55%. The results indicate that Immunozyme Mycobacterium test is valuable tool in tuberculosis diagnosis especially in case when a technical equipment is wanting.

[Generation of reactive oxygen species by peripheral blood neutrophils in patients with pulmonary tuberculosis]. / Wytwarzanie reaktywnych form tlenu przez neutrofile krwi obwodowej chorych na gruzlice pluc.

Oxygen metabolism of neutrophils was measured using chemiluminescence method in patient with pulmonary tuberculosis. Patients were included into three groups. Group I--20 patients (10 men, 10 women) aged 24-74 years (mean 48.3 years) with pulmonary tuberculosis BK(+). Group II--20 patients (15 men, 5 women) aged 19-67 years (mean 45.1 years) with pulmonary tuberculosis BK(0). The control group consisted 16 clinically healthy persons (12 men, 4 women) aged 28-59 years (mean 42.5 years). Blood samples (5 ml) were collected for examination from cubital vein early morning before breakfast. A luminometer 1251 coupled with an IBM PC AT compatible computer was used for the measurement of chemiluminescence. The chemiluminescence of non-stimulated neutrophils and those stimulated by a receptor stimulus formyl-Met-Leu-Phe (fMLP) as well as an extrareceptor stimulus phorbol myristate acete (PMA) was measured. The results of our study demonstrated that chemiluminescence of non-stimulated as well as stimulated by fMLP and PMA neutrophils was lower in comparison to the values of chemiluminescence in the control group.

[Estimation of plasma malonyl dialdehyde concentration in patients with pulmonary tuberculosis]. / Ocena stezenia dialdehydu malonowego w osoczu chorych na gruzlice pluc.

Plasma malonyl dialdehyde (MDA) concentration was determined by Placer method in patients with pulmonary tuberculosis before and during treatment period with tuberculostatic drugs. Patients were divided into three groups. Group I comprised 20 patients (10 men and 10 women) aged 24-74 years (mean 48.3 years) with pulmonary tuberculosis BK(+). Group II comprised 20 patients (15 men and 5 women) aged 19-67 years (mean 45.1 years) with pulmonary tuberculosis BK(0). The control group consisted of 16 clinically healthy persons (12 men and 4 women) aged 28-59 years (mean 42.5 years). In patients blood samples (5 ml) were collected for examination from cubital vein before, after 1-month and 2-month treatment period with tuberculostatic drugs. In the control group blood samples were collected from cubital vein once. Results of our study showed that plasma MDA concentrations in patients with pulmonary tuberculosis, both before and during treatment period with tuberculostatic drugs, were significantly higher, in comparison to the control group.

Pentoxifylline decreases neutrophil respiratory bursts in patients with stable angina.

We estimated the effect of pentoxifylline (PTX) on the respiratory burst (examined by chemiluminescence method) of unprimed and primed neutrophils with tumor necrosis factor-alpha (TNF-alpha) in patients with stable angina pectoris. Chemiluminescence of non-stimulated as well as formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol myristate acetate (PMA) stimulated neutrophils was measured. We studied 45 patients with stable angina subjected to percutaneous transluminal coronary angioplasty (PTCA) procedure, who were randomly divided into two groups. The study group consisted of 24 patients who were administered pentoxifylline orally, and the control group consisted of 21 patients without pentoxifylline administration. Blood samples for examination were collected from the coronary sinus and peripheral vein just before the PTCA procedure. Pentoxifylline decreased the respiratory burst of non-stimulated and fMLP-stimulated neutrophils without affecting the chemiluminescence of PMA stimulated neutrophils. Moreover, pentoxifylline diminished the chemiluminescence non-stimulated and stimulated by fMLP but not by PMA of TNF-alpha primed neutrophils. We presume that administration of PTX in stable angina patients may have a beneficial effect.

[The effect of pentoxifylline on oxidative metabolism in neutrophils primed with tumor necrosis factor alpha in patients with stable angina pectoris]. / Wplyw pentoksyfiliny na metabolizm tlenowy neutrofilów preaktywowanych przez czynnik martwicy nowotworów alfa u chorych ze stabilna dusznica bolesna.

In 14 patients with stable angina pectoris we examined the effect of pentoxifyline (PTX) on oxidative metabolism of TNF-alpha-priming neutrophils. The control group consisted of 21 patients with stable angina pectoris without pentoxifylline administration. Blood samples for examination were taken from basilic vein (peripheral blood) and coronary sinus immediately before PTCA procedure. In PTX-group was found the significant decrease in spontaneous CL of TNF-alpha-priming neutrophils from coronary sinus blood (1231.0 +/- 119.4 mV x min), in comparison to the control group (1374 +/- 124.4 mV x min). In PTX-group was found the significant decrease in fMLP stimulated CL of TNF-alpha-priming neutrophils from peripheral blood (4219.0 +/- 707.2 mV x min) and coronary sinus blood (4322.0 +/- 664.4 mV x min), in comparison to the control group (5248.0 +/- 595.8 mV x min and 4973.0 +/- 536.5 mV x min; respectively). There were no differences between both groups in PMA stimulated CL of TNF-alpha-priming neutrophils.