Fatty acid-binding capacity of cytosolic proteins of various rat tissues: effect of postnatal development, starvation, sex, clofibrate feeding and light cycle.

Fatty acid-binding capacity of dealbuminized, delipidated cytosolic proteins from rat tissues was studied with a radiochemical binding assay. Oleate-binding capacity ranges from 1.6 to 4.4 pmol/micrograms cytosolic protein in liver, heart, kidney, adrenal, brain, skeletal muscle and diaphragm. Differences in binding affinity indicate the presence of different fatty acid-binding proteins in these tissues. No change in fatty acid-binding protein content of heart and liver cytosol was observed during postnatal development up to 70 days. Starvation did not affect the fatty acid-binding capacity of heart cytosol, but increased the oleate-binding capacity in liver cytosol. Sex-related differences of binding by heart and liver cytosolic proteins were found with oleate, but not with palmitate. Fatty acid-binding capacity of liver and heart cytosol did not show marked diurnal variation. Clofibrate treatment had different effects on the oleate-binding capacity of cytosolic proteins: an increase in liver and kidney, no change in skeletal muscle and a decrease in heart. The results are discussed in relation to data concerning fatty acid oxidation.

Waterborne Yersinia enterocolitica in the midwest United States.

One hundred forty strains of waterborne Y. enterocolitica were isolated from Wisconsin and Colorado rivers, lakes and wells between October 1974 and March 1976- Direct-plating of unconcentrated water specimens on deoxycholate-citrate-mannitol (Y-M) agar resulted in 89 isolates. Prolonged incubation of specimens in cooked meat broth produced 51 additional strains. Most organisms were indole-positive, Niléln biotype 1 with 24% belonging to the rhamnophilum subgroup. Twenty-three serotypes were represented with 13% reacting as 0:4,32/33, 43% of the isolates could not be serotyped. Twelve organisms were phagetyped as either XO or XZ.