RESUMO
This study describes the epidemiological association of 3 putative genes for virulence of uropathogenic Escherichia coli; uropathogenic specific protein (usp), a Vibrio cholerae zot gene homologue; IrgA homologue adhesin (iha), a nonhemagglutinating adhesin; and iroN(E. coli), a catechole siderophore receptor homologue. We compared the relative frequency in urinary tract infection (UTI) isolates (n=508), compared with non-UTI isolates (n=416). iroN(E. coli) occurred 2.1-3.6 times more frequently in UTI isolates than in rectal isolates (P=1.1x10-18 to P=2.7x10-5) and was associated with several uropathogenic virulence genes found on pathogenicity islands. usp occurred more frequently in isolates from patients with pyelonephritis (P=3.6x10-9), in periurethral isolates (P=.001), and in isolates from patients with UTI who were aged 40-65 years (P=.004), when compared with the rectal isolates; iha was not associated with UTI in this study.
Assuntos
Proteínas de Bactérias/genética , Infecções por Escherichia coli/epidemiologia , Proteínas de Escherichia coli/genética , Escherichia coli/genética , Genes Bacterianos , Receptores de Superfície Celular , Infecções Urinárias/microbiologia , Vibrio cholerae/genética , Adolescente , Adulto , Idoso , Escherichia coli/patogenicidade , Feminino , Finlândia/epidemiologia , Humanos , Pessoa de Meia-Idade , Razão de Chances , Prevalência , Pielonefrite/microbiologia , Reto/microbiologia , VirulênciaRESUMO
A total of 868 isolates was screened from seven different collections of organisms from previous studies - pyelonephritis in children aged 1-24 months; first, second and recurring urinary tract infection (UTI) in women aged 18-39 years; UTI in women aged 40-65 years and peri-urethral and faecal isolates from women aged 18-39 years - for the presence of 10 potential Escherichia coli UTI virulence genes. Previously reported differences between the frequency of these genes in UTI compared with faecal isolates were confirmed and extended. A single virulence signature (strains containing aer, kpsMT, ompT, fim and papGAD) occurred in 29% of the pyelonephritic isolates, but in no more than 11% of the other collections. Peri-urethral isolates were found to have frequencies of these 10 genes that differed from those found for both UTI and faecal isolates.
Assuntos
Escherichia coli/genética , Escherichia coli/patogenicidade , Fezes/microbiologia , Genes Bacterianos , Uretra/microbiologia , Infecções Urinárias/microbiologia , Adolescente , Adulto , Idoso , Feminino , Humanos , Lactente , Pessoa de Meia-Idade , Virulência/genéticaRESUMO
Chromosomal genotypes of Escherichia coli isolates from blood, urine and faeces of infants with urosepsis were studied to find possible clonality of the isolates. The isolates were analysed by PCR for class I, II and III alleles of the pyelonephritis-associated adhesin gene papG. The macrorestriction profiles of the papG-positive isolates were analysed by pulsed-field gel electrophoresis and their O serogroups were determined. Genetically identical E. coli isolates from the blood, urine and faeces of the same infant were found in 8 of 10 infants. This finding confirmed the results of previous phenotypic studies that the reservoir of pyelonephritogenic E. coli is indeed the colon.
