Retalho deslocado coronalmente com a utilização de enxerto conjuntivo subepitelial e Emdogain para recobrimento radicular ­ controle clínico de cinco anos / Coronally repositioned flap with subepithelial connective tissue graft and Emdogain for root coverage ­ a 5 year follow-up case report

Uma situação desafiadora na Odontologia é a recessão gengival. Seu tratamento deve ser capaz de promover um completo recobrimento do defeito da recessão com mínima profundidade de sondagem pós-tratamento, além de promover resultados de cor e textura na área recoberta compatível com a dos tecidos moles adjacentes. Algumas situações clínicas prévias devem ser observadas quando do exame do paciente, dentre elas: a etiologia da lesão, o grau de classificação e a existência de lesões cariosas na raiz exposta. Uma abordagem previsível para o recobrimento radicular é o retalho deslocado coronalmente associado ao enxerto de tecido conjuntivo subepitelial. As proteínas derivadas da matriz do esmalte vêm sendo utilizadas associadas a técnicas de recobrimento com o intuito de aumentar o potencial regenerativo da região. Neste relato, com controle clínico de cinco anos, foi realizada a recuperação do tecido gengival perdido na região do dente 31, com o uso da técnica do retalho deslocado coronalmente associado ao enxerto de tecido conjuntivo subepitelial e ao Emdogain. Esta associação resultou em um importante ganho de inserção, além da melhora estética na região para o paciente.

A challenging situation in dentistry is gingival recession. Its treatment should be able to promote a complete coverage of the recession defect with minimum post-treatment probing depth, in addition to promoting color and texture results in the covered area compatible with the adjacent soft tissue. Some previous medical conditions must be observed when examining the patient, among them the etiology, the degree of classifi cation and the existence of caries on the exposed root. A predictable approach to root coverage is the coronally repositioned flap associated with subepithelial connective tissue grafting. The enamel derived matrix has been associated with coverage techniques in order to increase the regenerative potential. In this 5 year case report, the lost gingival tissue was recovered at the region of tooth 31 using a coronally advanced fl ap associated to STG and Emdogain. There were esthetic improvements and better attachment levels observed in the clinical results.

In vitro evaluation of the biological effect of SOFAT on osteoblasts.

Osteoclastogenesis is regulated by osteoblasts especially through the production of receptor activator of nuclear factor kappa-B ligand (RANKL). Immune cells present in inflamed tissues markedly increase this process by upregulating RANKL directly or by secreting proinflammatory cytokines, which stimulate RANKL expression by osteoblasts. A novel T-cell-secreted cytokine, termed secreted osteoclastogenic factor of activated T cells (SOFAT) was recently described. To better understand how SOFAT affects bone metabolism, we investigated its effect on osteoblastic cells. We demonstrate here that SOFAT did not influence MC3T3 cells viability and proliferation, evaluated by trypan blue exclusion and MTT tests, respectively. SOFAT stimulated the secretion of IL-6, IL-10 and GM-CSF in MC3T3 cells, as shown by the analysis of an inflammatory cytokines ELISA array. The upregulation of the corresponding genes was checked by qPCR. Both RANKL mRNA and protein levels did not significantly change in the presence of SOFAT, evaluated by qPCR and western blotting, respectively. In addition, analysis of a PCR array for IL6/STAT3 pathway demonstrated that SOFAT induced the expression of BCL2, IL1B, IL10, IL22, IL2RA, IL4, IL6, TNFSF10 and PIAS3, while IL2, IL21, CD4, CSF3R and TNF were repressed. Our results confirm that the SOFAT mechanism of action is RANKL-independent and indicate that, by co-opting osteoblasts to increase the production of osteoclastogenic cytokines, SOFAT may exacerbate inflammation and support osteoclast formation and bone destruction.

Instalação e provisionalização imediata de implantes após exodontia em dentes com reabsorção externa / Immediate installation and provisionalization of implants after extraction of teeth with external resorption

Vários fatores podem levar à perda dentária, entre eles a reabsorção externa das raízes. Para este, entre outros casos, a reposição dos dentes perdidos com a instalação imediata de implantes e provisionalização imediata tem sido um procedimento com bom prognóstico. Fatores como extração minimamente traumática, manutenção das cristas ósseas proximais e das tábuas ósseas vestibular e lingual, estabilidade primária do implante e a confecção de provisório imediato livre de contatos oclusais são parâmetros importantes para a previsibilidade do caso. Esta técnica tem sido descrita por vários autores desde a década de 90 com alto índice de sucesso, otimizando a reabilitação dos pacientes com menor tempo de espera e a preservação da arquitetura dos tecidos gengival e ósseo. Este trabalho apresenta o relato de dois casos clínicos de exodontias de dentes com reabsorção externa, previamente submetidos ao tracionamento ortodôntico, seguidas de instalação imediata de implantes e provisionalização imediata, com acompanhamento clínico e radiográfico de 7 anos...

Several factors can lead to tooth loss, including external root resorption. In these cases, among others, the replacement of missing teeth with immediate implant placement and provisionalization has been a procedure with good prognosis. Factors including minimally traumatic extraction, maintenance of proximal bone crests and buccal and lingual bone plates, primary implant stability and immediate provisionalization free of occlusal contacts are strictly important for success and good long-term result. This technique has been described since the 90s with a high success rate. It can optimize the rehabilitation with a shorter waiting time maintaining of bone and gingival contours. This article describes a 7-year radiographic and clinical follow-up of two extraction cases of teeth with external root resorption followed by immediate implant placement and immediate provisionalization...

Implante imediato com provisionalização e utilização da coroa natural do elemento extraído / Immediate dental implant after tooth extraction using the natural dental crown as a provisional restoration. A case report

A implantação imediata após a exodontia com a confecção de um elemento protético provisório tem se tornado uma proposta viável, amplamente documentada na literatura e que diminui consideravelmente o tempo de tratamento e a quantidade de intervenções, contribuindo para maior satisfação do paciente. A observação de fatores relevantes da estrutura óssea remanescente e do contorno gengival da região deve ser considerada, com o objetivo de otimizar o prognóstico. Outras considerações quanto ao desenho e superfície do implante, conduta cirúrgica e protética também devem ser criteriosamente analisadas. O objetivo deste trabalho foi demonstrar, por meio de um relato de caso clínico, a utilização de implante imediato após a exodontia de um dente perfurado endodonticamente, seguido de provisionalização imediata, utilizando-se da coroa do dente extraído como elemento provisório, com acompanhamento de seis meses.

Immediate implantation after extraction followed by a provisional prosthetic element has become a viable proposition, widely documented in the literature and which significantly reduces treatment time and number of interventions, contributing to greater patient satisfaction. The observation of relevant factors of the remaining bone structure and gingival contour of the region must be considered in order to optimize the prognosis. Other considerations such as implant design and surface, surgical and prosthetic protocols should also be carefully analyzed. The objective of this paper is to demonstrate through a clinical case report, the use of immediate implant after extraction of a tooth endodontically perforated, followed by immediate provisionalization, using the crown of the extracted tooth as provisional element representing the 6-month follow-up report.

Secreted osteoclastogenic factor of activated T cells (SOFAT), a novel osteoclast activator, in chronic periodontitis.

A novel activated human T cell-secreted cytokine, referred as secreted osteoclastogenic factor of activated T cells (SOFAT), that induce osteoclastogenesis in a RANKL-independent manner was recently described. This study evaluated the role of SOFAT in periodontal tissues and periodontitis. Gingival biopsies were harvested from systemically healthy non-periodontitis (n=15) and chronic periodontitis patients (n=15). The mRNA and protein levels of SOFAT were measured by qPCR and by enzyme-linked immunosorbent assay, respectively. Moreover, RAW 264.7 cells were cultured with SOFAT or Receptor activator of nuclear factor-kB ligand (RANKL) and stained for tartrate-resistant acid phosphatase (TRAP). Also, mice received a palatal injection between the first and second upper molar of SOFAT (100 ng/ml) or saline solution (0.9%). The upper jaw was removed, histologically processed and stained with hematoxilin and eosin to observe the presence of osteoclast-like cells. The mRNA and protein levels of SOFAT were significantly higher in the gingival tissue of the periodontitis group when compared to non-periodontitis one (p<0.05). In addition, SOFAT potently induced TRAP-positive multinucleated cell formation by RAW 264.7 cells as well as induced the formation of osteoclast-like cells in the periodontal ligament in mice. The present study demonstrated that SOFAT may play an important role in periodontitis.