Effect of extracellular matrix and dental pulp stem cells on bone regeneration with 3D printed PLA/HA composite scaffolds.

The demand for bone grafting procedures in various fields of medicine is increasing. Existing substitutes in clinical practice do not meet all the criteria required for an ideal bone scaffold, so new materials are being sought. This study evaluated bone regeneration using a critical-size Wistar rat's calvarial defect model. 12 male and 12 female rats were evenly divided into 3 groups: 1. Negative and positive (Geistlich Bio-Oss®) controls; 2. polylactic acid (PLA) and PLA/hydroxyapatite (HA); 3. PLA/HA cellularised with dental pulp stem cells (DPSC) and PLA/HA extracellular matrix (ECM) scaffolds. PLA/HA filament was created using hot-melt extrusion equipment. All scaffolds were fabricated using a 3D printer. DPSC were isolated from the incisors of adult Wistar rats. The defects were evaluated by micro-computed tomography (µCT) and histology, 8 weeks after surgery. µCT revealed that the Bio-Oss group generated 1.49 mm3 and PLA/HA ECM 1.495 mm3 more bone volume than the negative control. Histology showed a statistically significant difference between negative control and both (Bio-Oss and PLA/HA ECM) groups in rats of both genders. Moreover, histology showed gender-specific differences in all experimental groups and a statistically significant difference between cellularised PLA/HA and PLA/HA ECM groups in female rats. Qualitative histology showed the pronounced inflammation reaction during biodegradation in the PLA group. In conclusion, the bone-forming ability was comparable between the Bio-Oss and PLA/HA ECM scaffolds. Further research is needed to analyse the effects of ECM and PLA/HA ratio on osteoregeneration.

Principles of high-frequency ultrasonography for investigation of skin pathology.

Ultrasonography is a valuable diagnostic tool widely used in medicine. During the last three decades, this non-invasive skin imaging method has been extended to dermatology. High-frequency ultrasonography with higher than 20MHz scanners is well-established for measuring tumour thickness and skin thickness when treating inflammatory skin diseases such as scleroderma or psoriasis. High-frequency ultrasonography has become extremely helpful for the preoperative assessment of skin melanoma. The correlation between ultrasonic and histological measurements of melanomas thickness is significantly similarly good using transducers of 20, 75 or 100MHz frequency (r range from 0.895 to 0.99) and better compared with transducers of 7.5MHz frequency (r=0.76). The preoperative sonographically estimated thickness of skin melanoma is sometimes overestimated, because of an underlying inflammatory infiltrate and other reasons. Assessment of skin melanoma thickness using transducers of 100MHz frequency has better agreement with histology, compared with ultrasonography with 20MHz transducers. However, the ultrasonic penetration depth is limited to 1.5mm in case of 100MHz. The newer ultrasonic techniques such as high-frequency ultrasonography and colour Doppler sonography could be used for assessment of the tumour vascularization and its metastatic potential. The wide variety of diagnostic information provided by high-frequency ultrasonography undoubtedly improves the management of oncological and inflammatory skin conditions and underlines its essential position in dermatological practice.

Ultrasonic fields radiated through matching layers with nonparallel boundaries.

In the case of ultrasonic measurements in aggressive media piezoelectric elements of ultrasonic transducers are separated from a medium by thick protective layers, which may posses nonparallel front and back surfaces. This enables to reduce significantly the amplitude of multiple reflections, but the structure of the ultrasonic field radiated through a layer with nonparallel boundaries becomes complicated. The main objective of this paper is to present a method suitable for simulation of ultrasonic fields radiated through a layer with nonparallel boundaries in a transient mode. The proposed simulation method is based on the transformation of a multilayered medium into a virtual one without internal boundaries, equivalent to the actual medium from the point of a view of the relative times of arrival of direct and edge waves. The simulated ultrasonic fields in water are compared with the measurement results and a good correspondence between calculated and measured fields is obtained.

Evaluation of diffraction errors in precise pulse-echo measurements of ultrasound velocity in chambers with waveguide.

Ultrasound velocity measurements in medicine and biology usually are performed using relatively small measurement chambers. When the pulse-echo method is used, the presence of the reflector close to the transducer can cause essential diffraction errors. These errors may be reduced using an additional buffer rod as a waveguide between the transducer and the measurement chamber. The objective of the presented work was analysis of diffraction errors in measurement chambers with a buffer rod. The work was performed in two steps. In the first stage propagation of transient ultrasonic waves in a buffer rod was analysed using an axisymmetric finite element model. This approach enables all dimensions of the measurement chamber and the waveguide to be taken into account, but is less accurate in the time domain. In the second step the absolute values of diffraction errors were evaluated using a mixed analytic-numeric disk shaped transducer diffraction model. In this case only the dimensions of the waveguide and measurement chamber along the wave propagation direction were taken into account. Diffraction errors were calculated by simulating small changes of ultrasound velocity in the liquid under investigation. The simulation performed allowed optimisation of the dimensions of the measurement chamber and a buffer rod thus minimising measurement errors.