RESUMO
The present study was undertaken for the immuno-diagnosis of Haemonchus contortus infection in sheep by counter immuno-electrophoresis (CIEP) using crude somatic antigen. Adult worms of H. contortus were found in 57 out of 100 abomasums screened. A total of 250 serum samples which includes, 100 serum from local abattoir in and around Shimoga region from the sheep from which the abomasums were collected for H. contortus worms and 150 serum from the migratory sheep were collected to detect the circulating antibody of H. contortus by CIEP using crude whole worm somatic antigen. Out of 100 abomasums screened, 18 were heavily infected with adult worms of H. contortus and their serum samples showed positive reaction with clear thick precipitin line with crude somatic antigen of H. contortus by CIEP and 39 samples showed light precipitin line, which were moderately infected with adult worms of H. contortus during necropsy. However, the 12 serum samples showed positive reaction with clear precipitin line, but their abomasums did not show H. contortus and the remaining 31 were not harbouring any adult worms of H. contortus and no precipitin line observed by CIEP. The sensitivity and specificity of CIEP was found 100 and 72.09 %, respectively. In the present study, 62 % sero-prevalence was observed with CIEP among 150 serum samples from migratory sheep screened for antibodies of H. contortus using crude whole worm somatic antigen of H. contortus.
RESUMO
In the present study, post-mortem was conducted on a female elephant aged about 37 years died at Rajeev Gandhi National Park, Hunsur, Mathigoodu Elephant Camp, Karnataka state. The animal suffered with diarrhoea, anorexia, dehydration and was unable to walk for about one week before death and was treated with antibiotics and fluid therapy for three days. The post-mortem examination revealed that, the gastric mucosa was severely congested, hyperaemic and numerous stomach bots attached to the mucosa. The bots were recovered from the gastric mucosa and processed for species identification. The posterior spiracles of the bots showed three longitudinal parallel slits in each spiracle, the abdominal segments had a row of belt like triangular shaped spines and the anterior end had two powerful oral hooks with cephalo-pharyngeal skeleton. Based on the above said morphological characters, the bots were identified as Cobboldia elephantis. This seems to be the first report of C. elephantis in free range wild elephant from Karnataka state.
RESUMO
The checkered keelback is also known as Asiatic water snake and is common species of non-venomous snake known to occur in India. The present study report the occurrence of Ascaridia galli infection in a checkered keelback snake, which was found dead near Veterinary College hostel premises. The post-mortem examination of the snake revealed catarrhal exudate without ingesta in the stomach and showed gastric ulcerations with edema. Large numbers of round worms were packed in the lumen of small intestine with petechial haemorrhages and necrotic enteritis. The nematode parasites were recovered and processed for identification. Based on morphological characters, the worms were identified as A. galli and the intestinal contents of the snake showed A. galli eggs.
