Effect of 3D printer, implant analog and angulation on the accuracy of analog position in implant casts.

OBJECTIVES: To evaluate the accumulative effect of 3D printer, implant analog systems, and implant angulation on the accuracy of analog position in implant casts. METHODS: A reference cast, presenting a case of a three-unit implant-supported prosthesis, was scanned with a coordinate measurement machine, producing the first reference data set (CMM, n = 1). The second reference data set (n = 10) was prepared using an intraoral scanner (IOS) (Trios4). Test quadrant casts were produced using three DLP type 3D printers, Max (MAX UV385), Pro (PRO 4K65 UV), and Nex (NextDent 5100), and three implant analog systems, El (Elos), Nt (Nt-trading), and St (Straumann) (n = 90). Stone casts were also produced via analog impressions (Stone, n = 10). After digitization, the accuracy of 3D distance, local angulation (angle between implants) and global angulation (angle between the implant center axis and an axis perpendicular to the global plane) was evaluated by comparing the reference (CMM, IOS), test (3D print), and control (Stone) groups using metrology software. Data were statistically analyzed using three-way ANOVA and Tukey`s tests (α = 0.05). RESULTS: IOS was truer in 3D implant distance and more precise in capturing local angulation than Stone (p ≤ 0.05). Other measurements were similar between both groups (p > 0.05). The amount of error introduced in the workflow by IOS and 3D printing was mostly similar (p > 0.05). 3D printed casts had similar or even higher accuracy than Stone group (p > 0.05). In most cases, higher trueness was achieved when using PRO 4K65 UV 3D printer and Elos implant analog system (p ≤ 0.05). CONCLUSION: 3D printer, implant analog system, and implant angulation have a significant effect on the accuracy of analog position in implant casts. Limited-span implant-supported cases could be reproduced digitally with similar accuracy as conventional methods. CLINICAL SIGNIFICANCE: A fully digital workflow with a carefully selected 3D printer and implant analog system can increase the accuracy of digitally produced implant casts with comparable accuracy to conventional workflow.

Effect of Different Intraoral Scanners on the Accuracy of Bite Registration in Edentulous Maxillary and Mandibular Arches.

OBJECTIVES: The objective of this study was to use in vitro models to examine the bite registration accuracy of four different intraoral scanners (IOS) for edentulous maxillary and mandibular arches. The objective was to assess the trueness and precision of the IOS and determine if there were significant differences between them. METHODS: An Asiga Max UV 3D printer was used to print maxillary and mandibular edentulous models based on the shape of Frasaco models (artificial dental arch models). Four dental implants were placed symmetrically in both models using Straumann BLT RC implants. Digital impressions were taken with Primescan, Trios 3, Trios 4, and Medit i500 intraoral scanners (n = 10 for each IOS). Digital bite registrations were made, and scanning data was exported in STL format. The accuracy of the interarch distance (the distance between the metrological spheres attached to the mandibular and maxillary models) was estimated for each IOS. RESULTS: The results showed significant differences in trueness and precision between different IOS (p <.05), except Medit i500 and Trios 3 (p >.05). Primescan provided the most accurate results, followed by Medit i500, Trios 3, and Trios 4, respectively. CONCLUSIONS: within the limitations of this study, the IOS type affects the accuracy of interocclusal bite registration in in vitro design. Only Primescan achieved clinically acceptable accuracy for the interocclusal recording of edentulous arches. CLINICAL RELEVANCE: The comparison of the accuracy of bite registration between different intraoral scanners will help increase the efficiency of the clinical application of digitalized interarch registration.

Trueness of Crown Preparation Dies in Dental Models: An In Vitro Assessment of Digital and Analog Workflows.

PURPOSE: To assess crown die trueness using additive manufacturing (AM) based on intraoral scanning (IOS) data and compare it with stone models. MATERIALS AND METHODS: Crown dies with four finish line types- equigingival shoulder (SAE), subgingival shoulder (SAS), equigingival chamfer (CAE), and subgingival chamfer (CAS)-were incorporated into a reference model and scanned with a coordinate measurement machine (CMM; n = 1 scan). Trios4 (3Shape) scans generated a second reference dataset (IOS; n = 10 scans). Using scans, crown dies were produced with two different 3D printers (MAX UV385 [Asiga] and NextDent 5100 [3DSystems]; n = 10 per system). Stone dies were created from conventional impressions (n = 10). Specimens were digitized with a laboratory scanner (E4, 3Shape). Trueness was evaluated with Geomagic Control X (3DSystems). Data analysis was done using Shapiro-Wilk, Levene, ANOVA, and t tests (α < .05). RESULTS: All crown dies fell within the clinically acceptable trueness range (150 µm). IOS exhibited significantly lower (P < .05; Δ ≤ 21.7 µm) or similar trueness compared to stone models. Asiga dies demonstrated similar and NextDent significantly lower marginal trueness than IOS (P < .05; Δ ≤ 57.3 µm). Most AM margin areas had significantly lower trueness than stone (P < .001; Δ ≤ 57.2 µm). Asiga outperformed NextDent (P < .001). Shoulder trueness surpassed chamfer in optical scans (P = .01). Finish line design and gingiva location did not have a significant impact on AM and stone models (P > .05). CONCLUSIONS: Combining IOS and AM achieves clinically acceptable crown die trueness for single molar teeth. The choice of AM device is critical, with Asiga outperforming NextDent. Finish-line design has an impact on optical scans. Finish-line design and marginal gingiva location have little effect on AM trueness.

Effect of additional reference objects on accuracy of five intraoral scanners in partially and completely edentulous jaws: An in vitro study.

STATEMENT OF PROBLEM: The effect of additional reference objects on the accuracy of different intraoral scanners for partially and completely edentulous patients has not been investigated sufficiently. PURPOSE: The purpose of this in vitro study was to evaluate the effect of an additional reference object in the form of additional artificial landmarks on the trueness and precision of different intraoral scanners in partially and completely edentulous areas. MATERIAL AND METHODS: Partially and completely edentulous models with 2 and 4 implants (BLT, RC, Institut Straumann AG), respectively, were used in the study. For the digital scan, scan bodies (CARES Mono Scanbody) were attached, and reference data obtained by using industrial scanners. Ten digital scans of the same model were made with each intraoral scanner: PRIMESCAN, TRIOS 3, TRIOS 4, Carestream 3600, and Medit. Then, additional artificial landmarks were attached, and 10 more intraoral scans were made with each device. Computer-aided design files of the scan bodies were aligned to obtain 3-dimensional surfaces with reference and test scanners. Trueness and precision of distance, angulations, and vertical shift between scan bodies were estimated. The Mann-Whitney Wilcoxon or Student 2-sample t test was applied to estimate statistically significant differences between groups (α=.05). RESULTS: In the partially edentulous model, distance trueness mean ±standard deviation values ranged from -46.7 ±15.4 µm (TRIOS 3) to 392.1 ±314.3 µm (Medit) in models without additional artificial landmarks. When additional artificial landmarks were applied, trueness of distance mean ±standard deviation values ranged between -35 ±13 µm (TRIOS 4) and 117.7 ±232.3 µm (CARESTREAM). Trueness mean ±standard deviation values of angulation varied from -0.0 ±0.5 degrees (CARESTREAM) to 0.2 ±0.0 degrees (PRIMESCAN) without additional artificial landmarks and from 0.0 ±0.2 degrees (TRIOS 3) to 0.4 ±0.5 degrees (CARESTREAM) with additional artificial landmarks. Vertical shift trueness measurements varied from -108 ±47.1 µm (TRIOS 4) to 107.2 ±103.5 µm (Medit) without additional artificial landmarks and from -15.0 ±45.0 µm (CARESTREAM) to -86.9 ±42.1 µm (TRIOS 4) with additional artificial landmarks. The additional artificial landmark technique improved the trueness of all measured parameters for the 5 tested intraoral scanners. No statistically significant differences were found among models with or without additional artificial landmarks, except for Medit in all parameters and PRIMESCAN in angle measurements (P<.05). The best precision for distance was found with TRIOS 3 and with PRIMESCAN for angulation and vertical shift. Larger deviations were observed in the completely edentulous situation. The effect of additional artificial landmarks was limited when the accuracy parameters of digital scans were considered. CONCLUSIONS: Scans with and without additional artificial landmarks of partially edentulous conditions scanned by any of the intraoral scanners tested did not influence precision and trueness, except for Medit i500 in the distance and vertical shift parameters and CARESTREAM3600 in vertical shift. Precision and trueness of digital scans of completely edentulous areas were affected, except for Medit i500 for distance, PRIMESCAN and TRIOS 4 for angle, and all systems except TRIOS 4 for vertical shift precision.

A comparative biomechanical study of original and compatible titanium bases: evaluation of screw loosening and 3D-crown displacement following cyclic loading analysis.

PURPOSE: This study evaluated screw loosening and 3D crown displacement after cyclic loading of implant-supported incisor crowns cemented with original titanium bases or with three compatible, nonoriginal components. MATERIALS AND METHODS: A total of 32 dental implants were divided into four groups (n = 8 each): Group 1 used original titanium bases, while Groups 2-4 used compatible components. The reverse torque value (RTV) was evaluated prior to and after cyclic loading (1,200,000 cycles). Samples (prior to and after cyclic loading) were scanned with a microcomputed tomography (micro-CT). Preload and postload files were superimposed by 3D inspection software, and 3D crown displacement analysis was performed using root-mean-square (RMS) values. All datasets were analyzed using one-way ANOVA and Tukey's post hoc analysis. RESULTS: Significant variations were observed in the postload RTV, depending on the titanium base brand (P < .001). The mean postload RTVs were significantly higher in Groups 1 and 2 than in the other study groups. While evaluating 3D crown displacement, the lowest mean RMS value was shown in the original Group 1, with the highest RMS value occurring in Group 4. CONCLUSION: Within the limitations of this in vitro study and under the implemented conditions, it was concluded that the manufacturer brand of the titanium base significantly influenced screw loosening following the fatigue test and influenced 3D crown displacement after cyclic loading.

Error propagation from intraoral scanning to additive manufacturing of complete-arch dentate models: An in vitro study.

OBJECTIVES: To evaluate deviation propagation from data acquisition with an intraoral scanner to additive manufacturing of complete-arch dentate models. METHODS: A reference (Ref) mandibular dentate model having 5 precision spheres was scanned with a coordinate measurement machine equipped with a laser scanning head (ALTERA; Nikon) producing a Ni reference data set (n = 1). Digital impressions were taken of the Ref model with intraoral scanner (IOS) (Trios4; 3Shape) with Insane (T4_Imo) and Classic (T4_Cmo) scanning modes (each n = 10). T4_Imo scans were used as a second reference data set and to produce test models with two additive manufacturing (AM) devices (each n = 10): MAX UV385 (Asiga) and NextDent 5100 (3DSystems). As for the control group, dual viscosity vinyl polysiloxane impressions were taken of the Ref model and poured with Type IV dental stone (n = 10). All AM and stone models were scanned with a laboratory scanner (E4; 3Shape). Trueness and precision of linear (intermolar and intercanine width, arch length) and surface deviations were measured between reference (Ni, T4_Imo), test (T4_Cmo, AM), and control (stone) groups using best-fit alignments (Geomagic Control X; 3D Systems). The normality of data and differences between the groups were analyzed using Shapiro-Wilk, Levene's, Mann-Whitney U, Welch's t-test statistical analysis (p<0.05). RESULTS: The accuracy of the IOS impression was not significantly affected by the scanning mode (p>0.05). Stone models showed significantly better trueness than IOS impressions (p<0.05). AM models had higher trueness than IOS Imo digital impressions (p<0.05). The precision of AM models was comparable (linear, p>0.05) or lower (surface, p<0.05) than of IOS Imo digital impressions. Trueness was insignificantly different among the stone and AM models (p>0.05). Higher trueness was achieved by Max UV385 than with Nextdent 5100 (p<0.05). The majority of linear and all surface deviations of IOS impressions and AM models were below 200 µm. CONCLUSIONS: Within the limitations of this in vitro study, digital IOS impressions and AM models using the aforementioned equipment have acceptable accuracy for orthodontic and prosthodontic applications when complete-arch dentate records are used. CLINICAL SIGNIFICANCE: IOS and AM devices can have a significant influence on error propagation when applying digital workflow with complete-arch dentate models.

Permanent Magnet Tracking Method Resistant to Background Magnetic Field for Assessing Jaw Movement in Wearable Devices.

There is a large gap between primitive bruxism detectors and sophisticated clinical machines for jaw kinematics evaluation. Large, expensive clinical appliances can precisely record jaw motion, but completely restrain the patient for the duration of the test. Wearable bruxism detectors allow continuously counting and recording bites, but provide no information about jaw movement trajectories. Previously, we developed a permanent magnet and three-axis magnetometer-based method for wearable, intra-oral continuous jaw position registration. In this work, we present an effective solution of the two main drawbacks of the method. Firstly, a two-adjacent-magnetometer approach is able to compensate for background magnetic fields with no reference sensor outside of the system's magnetic field. Secondly, jaw rotational angles were included in the position calculations, by applying trigonometric equations that link the translation of the jaw to its rotation. This way, we were able to use a three-degree-of-freedom (3-DOF) magnetic position determination method to track the positions of the 5-DOF human masticatory system. To validate the method, finite element modeling and a 6-DOF robotic arm (0.01 mm, 0.01°) were used, which showed a 37% decrease in error in the average RMSE = 0.17 mm. The method's potentially can be utilized in small-scale, low-power, wearable intra-oral devices for continuous jaw motion recording.

In vitro and in vivo accuracy of full-arch digital implant impressions.

OBJECTIVES: The main objective of the study was to compare the accuracy of full-arch digital implant impressions for fixed dental prosthesis under in vitro and in vivo conditions. MATERIALS AND METHODS: Eight patients (five women and three men) with at least one edentulous arch and with 4-6 osseointegrated implants participated in this study. For each edentulous arch (n = 10), experimental screw-retained titanium bar with attached four scan bodies was fabricated. The bar containing four scan bodies was screw-retained intraorally on implants and scanned with Trios 3 intraoral scanner eight times (IOS group, in vivo). Then, the bar was attached to the master cast and scanned eight times again with the same intraoral scanner (MIOS group, in vitro). Finally, the bar with scan bodies was scanned 8 times with a laboratory scanner (reference). Precision and trueness were calculated for 3 distances and 3 angles between the scan bodies (1-2, 1-3, and 1-4) in IOS and MIOS groups. RESULTS: Precision and trueness for the largest distance (1-4) were found to be 44 ± 18 µm and 32 ± 19 µm for the IOS group and 31 ± 16 µm and 30 ± 14 µm for MIOS group, respectively. Precision and trueness for the angle between the most distant scan bodies (1-4) were 0.22 ± 0.14° and 0.18 ± 0.10° for the IOS group and 0.16 ± 0.11° and 0.07 ± 0.05° for MIOS group, respectively. CONCLUSIONS: Intraoral conditions moderately affected the precision and trueness of Trios 3 (3Shape) intraoral scanner. Results of in vitro accuracy studies cannot be directly transferred to the clinical field.

Accelerometry-Enhanced Magnetic Sensor for Intra-Oral Continuous Jaw Motion Tracking.

Currently available jaw motion tracking methods require large accessories mounted on a patient and are utilized in controlled environments, for short-time examinations only. In some cases, especially in the evaluation of bruxism, a non-restrictive, 24-h jaw tracking method is needed. Bruxism oriented, electromyography (EMG)-based devices and sensor-enhanced occlusal splints are able to continuously detect masticatory activity but are uninformative in regards to movement trajectories and kinematics. This study explores a possibility to use a permanent magnet and a 3-axial magnetometer to track the mandible's spatial position in relation to the maxilla. An algorithm for determining the sensor's coordinates from magnetic field values was developed, and it was verified via analytical and finite element modeling and by using a 3D positioning system. Coordinates of the cubic test trajectory (a = 10 mm) were determined with root-mean-square error (RMSE) of 0.328±0.005 mm. Possibility for teeth impact detection by accelerometry was verified. Test on a 6 degrees-of-freedom (DOF), hexapod-based jaw motion simulator moving at natural speed confirmed the system's ability to simultaneously detect jaw position and the impacts of teeth. Small size of MEMS sensors is suitable for a wearable intra-oral system that could allow visualization of continuous jaw movement in 3D models and could enable new research on parafunctional jaw activities.

Assessment of the morphology and dimensional accuracy of 3D printed PLA and PLA/HAp scaffolds.

INTRODUCTION: In complex clinical conditions when physiological bone regeneration is insufficient, there is a need to develop synthetic material-based scaffolds. The morphologic properties of porous scaffolds are of crucial importance. The dimensional accuracy of 3D printed scaffolds can be affected by a variety of factors. MATERIALS AND METHODS: Three groups of 3D printed scaffolds were investigated: PLA1 (pure polylactic acid) printed with an FDM Ultimaker Original printer, PLA2 and composite PLA/hydroxyapatite (PLA/HAp) scaffolds printed with a Pharaoh XD 20. PLA/HAp filament was created with hot-melt extrusion (HME) equipment. The morphology of the prepared scaffolds was investigated with SEM, micro-CT and superimposition techniques, gravimetric and liquid displacement methods. RESULTS: Layer heights of PLA1 scaffolds varied the most. PLA1 scaffold volume statistically significantly differed from PLA2 (p < 0.001) and PLA/HAp (p < 0.01) groups. Filament composition had no effect on the volumes of the scaffolds printed with the Pharaoh XD 20 printer (p > 0.05). The total porosity of printed PLA/HAp scaffolds deviated the least from the original STL model. CONCLUSIONS: This study showed that PLA/10% HAp filament fabricated with HME and printed with FFF 3D printer produced equal or even better accuracy of printed scaffolds than scaffolds printed with pure PLA filament. Further research is needed to analyze the effect of HAp on 3D scaffold morphology, accuracy, mechanical and biologic properties.

Accuracy of digital implant impressions with intraoral scanners. A systematic review.

AIM: The use of intraoral scanners (IOS) for making digital implant impressions is increasing. However, there is a lack of evidence on the accuracy of IOS compared with conventional techniques. Therefore, the aim of this systematic review was to collect evidence on the accuracy of digital implant impression techniques, as well as to identify the main factors influencing the accuracy outcomes. MATERIALS AND METHODS: Two reviewers searched electronic databases in November, 2016. Controlled vocabulary, free-text terms, and defined inclusion and exclusion criteria were used. Publications in English language evaluating the accuracy outcomes of digital implant impressions were identified. Pooled data were analysed qualitatively and pertinent data extracted. RESULTS: In total, 16 studies fulfilled the inclusion criteria: one in vivo and 15 in vitro studies. The clinical study concluded that angular and distance errors were too large to be acceptable clinically. Less accurate findings were reported by several in vitro studies as well. However, all in vitro studies investigating the accuracy of newer generation IOS indicated equal or even better results compared with the conventional techniques. Data related to the influence of distance and angulation between implants, depth of placement, type of scanner, scanning strategy, characteristics of scanbody and reference scanner, operator experience, etc were analysed and summarised. Linear deviations (means) of IOS used in in vitro studies ranged from 6 to 337 µm. Recent studies indicated small angle deviations (0.07-0.3°) with digital impressions. Some studies reported that digital implant impression accuracy was influenced by implant angulation, distance between the implants, implant placement depth and operator experience. CONCLUSIONS: According to the results of this systematic review and based on mainly in vitro studies, digital implant impressions offer a valid alternative to conventional impressions for single- and multi-unit implant-supported restorations. Further in vivo studies are needed to substantiate the use of currently available IOS, identify factors potentially affecting accuracy and define clinical indications for specific type of IOS. Data on Data on accuracy OF digital records, as well as accuracy of printed or milled models for implant-supported restorations, are of high relevance and are still lacking. Conflict-of-interest and funding statement: The authors state there is no conflict of interest.

Automated segmentation of transcranial sonographic images in the diagnostics of Parkinson's disease.

Images captured during routine clinical transcranial sonography (TCS) examination are of a low resolution, so can be confusing for diagnostic evaluations. Manual segmentation of brain structures (areas of the midbrain and substantia nigra (SN)) that are of special interest cause inter-observer and intra-observer variability, thus restricting the reliability of Parkinson disease (PD) diagnostics. This paper presents a new technique for automated segmentation applicable to low resolution sonographic images, and particularly to brain structures related to PD. The segmentation was performed by a modified shape-based active contour (AC) segmentation algorithm. In order to suppress the speckle noise and to improve the AC segmentation, a pre-processing technique based on the averaging of adjusted spatially varying TCS images is proposed. The latter technique was tested on clinical TCS images. The results of the automated segmentation were compared with the manual markings. Two experts on the 40TCS images performed these markings. The comparison showed that an automated method is effective when segmentation of the midbrain is performed (averaged overlap between regions obtained automatically and outlined manually was 73.10±7.45%). The results of the segmentation of the SN area showed that a sufficiently correct contour of this area could also be obtained, but the accuracy of the segmentation is related to the image quality. It should be emphasised that the main difficulty in evaluating the accuracy of automated segmentation of the SN was the indefinite "gold standard" (variation between the measurements of two experts with different experience was found). And, therefore, the diagnostic reliability of the proposed technique was inconclusive.

Correlations between digital planimetry and optical coherence tomography, confocal scanning laser ophthalmoscopy in assessment of optic disc parameters.

OBJECTIVE AND AIM: In routine clinical practice, laser methods for the evaluation of optic disc parameters are expensive and not accessible for all ophthalmologists; therefore, there is a need for less expensive technique. The aim of this study was to assess correlations between the parameters of the optic disc measured by digital planimetry (DP), optical coherence tomography (OCT), and confocal scanning laser ophthalmoscopy (CSLO) in healthy and glaucoma patients with the normal biometric parameters of the eye. MATERIAL AND METHODS: This case-control study enrolled 40 patients with glaucoma and 32 healthy patients with the normal biometric parameters of the eye. All subjects underwent full ophthalmologic examination, digital color optic disc photography, OCT, and CSLO at the same visit. The optic disc was morphometrically analyzed by DP, OCT, and CSLO. Seven optic disc parameters were evaluated. RESULTS: In the glaucoma group, the optic disc and cup areas (r=0.7-0.8, P<0.001) and cup-to-optic disc and rim-to-optic disc area ratios (r=0.7, P<0.001) measured by DP were strongly correlated with those measured by OCT and CSLO, while the horizontal and vertical cup-to-optic disc diameter ratios were found to be moderately correlated (r=0.6-0.7, P<0.001). In healthy patients, the optic disc and cup areas were strongly correlated (r=0.7-8.0, P<0.001). Significant differences in all optic disc parameters, except for the optic disc area, measured by DP, OCT, and CSLO were found between glaucoma and healthy patients. CONCLUSIONS: Strong correlations between the parameters of the optic disc measured by DP, OCT, and CSLO were found. There were significant differences in the parameters between healthy and glaucoma eyes measured using DP; therefore, this technique may be used for diagnosis, management, and screening of glaucoma.

Model based investigation of retinal vessel tortuosity as a function of blood pressure: preliminary results.

Tortuosity is one of parameters which describe a state of the eye fundus blood vessels. Tortuosity can be estimated from the detected vessels in optical fundus images. The increase in vessel tortuosity was observed in eyes of patients with advanced background diabetic retinopathy, papilloedema, even in some completely healthy eyes (in this case tortuosity does not change in time). Though many methods to estimate eye vessel tortuosity exist, dependencies between tortuosity and parameters of cardiovascular system are not fully explored. In this paper we studied whether different tortuosity estimation algorithms can detect the change of blood pressure in the cylindrical segment of the vessel modeled using finite elements method. In addition we studied how does one inhomogenity added inside the blood vessel influence the tortuosity and what are the relationships between the different tortuosity estimates and blood pressure? We found that even single inhomogenity of the vessel wall triggers the increase of tortuosity when inner blood pressure increases. The resulting dependencies among different tortuosity estimates and blood pressure are mostly nonlinear.

The use of information technologies for diagnosis in ophthalmology.

In 2003, a health IT programme for clinical decision support started in Lithuania. An initial goal was to create databases for ophthalmology images and to develop processing algorithms to extract diagnostically valuable information from images. We have investigated how vectors, consisting of the parameters derived from fundus images, are distributed and whether they form specific groups. When analysing the multidimensional patient data vectors, comprising all the 27 image parameters, it was impossible to separate the healthy eyes from the diseased ones. However, it was possible to simplify the system by eliminating redundant parameters and introducing new ones that represent a subset of parameters from the initial group. Thus it may prove possible to identify glaucoma using this system of parameters.

Web-based health services and clinical decision support.

The purpose of this study was the development of a Web-based e-health service for comprehensive assistance and clinical decision support. The service structure consists of a Web server, a PHP-based Web interface linked to a clinical SQL database, Java applets for interactive manipulation and visualization of signals and a Matlab server linked with signal and data processing algorithms implemented by Matlab programs. The service ensures diagnostic signal- and image analysis-sbased clinical decision support. By using the discussed methodology, a pilot service for pathology specialists for automatic calculation of the proliferation index has been developed. Physicians use a simple Web interface for uploading the pictures under investigation to the server; subsequently a Java applet interface is used for outlining the region of interest and, after processing on the server, the requested proliferation index value is calculated. There is also an "expert corner", where experts can submit their index estimates and comments on particular images, which is especially important for system developers. These expert evaluations are used for optimization and verification of automatic analysis algorithms. Decision support trials have been conducted for ECG and ophthalmology ultrasonic investigations of intraocular tumor differentiation. Data mining algorithms have been applied and decision support trees constructed. These services are under implementation by a Web-based system too. The study has shown that the Web-based structure ensures more effective, flexible and accessible services compared with standalone programs and is very convenient for biomedical engineers and physicians, especially in the development phase.

Development of teleconsultations systems for e-health.

Two prototype telemedicine systems have been developed: 1) a wireless system for status assessment of cardiology patients (WSCP), 2) a system for medical image management and teleconsultations (IMTS). The former system enables the patient to record an ECG on a personal digital assistant (PDA), view it and send it via a wireless connection. The doctor on duty is then able to view the received ECG and make appropriate decisions, also to apply for consultation by sending the received ECG to the PDA of a cardiology expert. The system logs all performed operations. The hardware used in the system consists of personal computers (PCs), PDAs, analog-digital converters, ECG sensors and GPRS modems. Software consists of programs for patients, doctors on duty, cardiology experts and administration, along with a central database. The second system is intended to be used by professional doctors for management of collected images and for teleconsultations via videoconferencing in order to obtain a second opinion. The system provides an integrated environment eliminating the need to jump between many applications. By using the system, doctors are able to acquire images from analog and digital cameras, process and enhance them, as well as upload them to local or remote databases. Doctors are also able to design custom database forms. The teleconsultation part of the system supports video and audio over ISDN and TCP-IP, using both a hardware codec (Zydacron Z360) and a software codec (based on MS Netmeeting). Images are sent from one client to another using the standard protocol T.120. Images become synchronized immediately upon reception by another client.