Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis.

Transitions in sexual system and reproductive mode may affect the course of sex chromosome evolution, for instance by altering the strength of sexually antagonistic selection. However, there have been few studies of sex chromosomes in systems where such transitions have been documented. The European tadpole shrimp, Triops cancriformis, has undergone a transition from dioecy to androdioecy (a sexual system where hermaphrodites and males coexist), offering an excellent opportunity to test the impact of this transition on the evolution of sex chromosomes. To identify sex-linked markers, to understand mechanisms of sex determination and to investigate differences between sexual systems, we carried out a genome-wide association study using restriction site-associated DNA sequencing (RAD-seq) of 47 males, females and hermaphrodites from one dioecious and one androdioecious population. We analysed 22.9 Gb of paired-end sequences and identified and scored >3000 high coverage novel genomic RAD markers. Presence-absence of markers, single-nucleotide polymorphism association and read depth identified 52 candidate sex-linked markers. We show that sex is genetically determined in T. cancriformis, with a ZW system conserved across dioecious and androdioecious populations and that hermaphrodites have likely evolved from females. We also show that the structure of the sex chromosomes differs strikingly, with a larger sex-linked region in the dioecious population compared with the androdioecious population.

Evolution of animal body plans: the role of metazoan phylogeny at the interface between pattern and process.

Comprehensive integrative studies are the hallmark of evolutionary developmental biology. A properly defined phylogenetic framework takes a central place in such analyses as the meeting ground for observation and inference. Molecular phylogenies take this place in many current studies on animal body plan evolution. In particular, 18S rRNA/DNA sequence analyses have yielded a new view of animal evolution that is often contrasted with a presumed traditional or classical view. First, I expose this traditional view to be a simplified historical abstraction that became textbook dogma. Second, I discuss how two recent important studies of animal body plan evolution, examining the evolution of the platyhelminth body plan and the evolutionary significance of indirect development and set-aside cells, have actively incorporated two problematic aspects of the newly emerging molecular view of animal evolution: incomplete and unresolved phylogenies.