RESUMO
An evaluation has been carried out of 13 commercial testosterone kits. The within- and between-assay imprecision was found to be unsatisfactory for some kits. Sensitivity and linearity were acceptable for all the kits but several showed concentration-dependent biases to the ALTM or GCMS values. The occurrence of anomalous results and changing performance characteristics of some of the direct kits is also presented.
Assuntos
Kit de Reagentes para Diagnóstico , Testosterona/sangue , Estudos de Avaliação como Assunto , Humanos , Plasma/análiseRESUMO
A questionnaire sent to laboratories in the UK and Ireland elicited information on acceptance or otherwise of values assigned to serum-based calibration materials for 14 analytes. Overall, 33% of assigned values were changed by users. The reasons for change, together with the degree of magnitude and direction of change, were studied for each analyte. Unidirectional changes made to assigned values were related to particular batches and manufacturers. Detailed information is presented for protein (Beckman RIIC Ltd.) on Astras, and sodium, bicarbonate and bilirubin on SMACs and SMAs (Technicon Instrument Co. Ltd.).
Assuntos
Análise Química do Sangue/normas , Laboratórios/normas , Humanos , Irlanda , Controle de Qualidade , Valores de Referência , Reino UnidoRESUMO
A scheme for kit evaluation in the individual clinical chemistry laboratory is presented. Recommendations for assessing accuracy, precision, and stability are included along with a scheme for preparing for the evaluation and writing the final report.
Assuntos
Química Clínica , Kit de Reagentes para Diagnóstico/normas , Estabilidade de Medicamentos , Estudos de Avaliação como Assunto , Estatística como AssuntoRESUMO
Eight amateur divers took part in crossover experiments to study the combined effects of ethanol (0.72 g/kg b.wt.) and hyperbaric air (4 and 6 ATA) on heart rate and body-sway movements. Body sway with open and closed eyes was measured in lateral and sagittal directions by a statometer device. In the alcohol condition, there was an initial increase in body sway corresponding to the acute phase of ethanol intoxication. At a mean blood alcohol concentration of 0.77 mg/ml, this increase in body sway was statistically significant (P less than 0.01) compared with the alcohol-free condition. At 90 min from start of drinking, body sway scores at 1 ATA were not significantly different from alcohol-free measurements. On raising the pressure to 4 and 6 ATA, increased body sway occurred in both alcohol and alcohol-free conditions and, moreover, the rate of increase was more extreme in the alcohol condition. Significant pressure-alcohol interactions were established, suggesting a potentiating action of alcohol on the increase in body sway induced by acute exposure to high pressures of air. Heart-rate measurements with and without alcohol were not significantly different, although increases in ambient pressure caused a drop in heart rate in both conditions.
Assuntos
Pressão do Ar , Intoxicação Alcoólica/fisiopatologia , Pressão Atmosférica , Frequência Cardíaca , Transtornos dos Movimentos/etiologia , Adulto , Intoxicação Alcoólica/complicações , Etanol/sangue , Humanos , MasculinoRESUMO
A method is described for the automation of the T3-uptake test using a discrete analyser. This enables 160 samples to be analysed in two hours at a cost of only 9p a sample. By making use of a simple correction procedure for differences in charcoal contact time and for radioactive carryover it is possible to get a within-batch precision of 2.5% and a between-batch precision of 4.6% for values within the normal range.
Assuntos
Tri-Iodotironina/sangue , alfa-Globulinas , Autoanálise/métodos , Humanos , Hipertireoidismo/sangue , Radioisótopos do Iodo , Testes de Função Tireóidea/métodos , Proteínas de Ligação a TiroxinaRESUMO
The relationship between changes of serum immuno-reactive cholecystokinin-pancreozymin (CCK-PZ) and serum immuno-reactive insulin has been studied after various stimuli. The oral administration of 5 percent glucose or magnesium sulphate and the intra-duodenal administration of olive oil were all followed by a rise of serum CCK-PZ. The serum insulin rose after 5 percent glucose and also showed a small but insignificant rise with olive oil. There was, however, no change of serum insulin after the ingestion of magnesium sulphate suggesting that CCK-PZ in isolation does not stimulate insulin release.
Assuntos
Colecistocinina/sangue , Insulina/sangue , Adulto , Glicemia/análise , Jejum , Feminino , Glucose , Humanos , Sulfato de Magnésio , Masculino , Óleos , Radioimunoensaio , Estimulação QuímicaRESUMO
In a group of healthy young male volunteers a worsened tolerance to oral glucose in the afternoon after a four-hour fast was reversed by a 12-hour fast. The latter also produced consistent mild hypoglycaemia from two and a half to four hours after the glucose loading irrespective of the time of day.
Assuntos
Glicemia/análise , Ritmo Circadiano , Jejum , Adulto , Teste de Tolerância a Glucose , Humanos , Masculino , Fatores de TempoRESUMO
The method of estimating serum thyroxine by competitive binding analysis has been examined stage by stage. The extraction procedures recommended by some authors are shown to extract protein as well as thyroxine, and so produce inaccurate results. A modified method is proposed which increases accuracy and precision.
