RESUMO
OBJECTIVES: To investigate the hair growth-promoting effect of Miscanthus sinensis var. purpurascens (MSP) flower extracton on in vitro and in vivo models. METHODS: MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells (hDPCs) in vitro at the dose of 3.92-62.50 µg/mL and hair growth of C57BL/6 mice in vivo at the dose of 1000 µg/mL. The expression of transforming growth factor ß1 (TGF-ß1), hepatocyte growth factor (HGF), ß-catenin, substance P was measured by relative quantitative realtime polymerase chain reaction. Histopathological and immunohistochemical analysis were performed. RESULTS: MSP (7.81 µg/mL) down-regulated TGF-ß1 and up-regulated HGF and ß-catenin in hDPCs (P<0.01). MSP (1000 µg/mL)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase. The number of mast cells was lower in the MSP-treated mice than in other groups (P<0.05 vs. NCS group). Substance P and TGF-ß1 were expressed in hair follicles and skin of the MSP group lower than that in negative control. Stem cell factor in hair follicles was up-regulated in the MSP-treated mice (P<0.01). CONCLUSIONS: The MSP flower extract may have hair growth-promotion activities.
Assuntos
Flores/química , Folículo Piloso/citologia , Extratos Vegetais/farmacologia , Poaceae/química , Estresse Psicológico/patologia , Animais , Antioxidantes/farmacologia , Contagem de Células , Proliferação de Células/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Feminino , Folículo Piloso/efeitos dos fármacos , Folículo Piloso/crescimento & desenvolvimento , Fator de Crescimento de Hepatócito/metabolismo , Humanos , Mastócitos/citologia , Camundongos Endogâmicos C57BL , Fosforilação/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Pele/metabolismo , Fator de Células-Tronco/metabolismo , Substância P/metabolismo , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/metabolismo , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , beta Catenina/metabolismoRESUMO
BACKGROUND: Geranium sibiricum L. has been used as a medicinal plant to treat diarrhea, bacterial infection, and cancer in Bulgaria, Peru, and Korea. However, its hair growth-promoting effect was not investigated so far. This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models. METHODS: Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). Hair-growth promoting effect was measured in animal model. Relative expression of interleukin-1, vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta 1 was determined by real time RT-PCR. Expression of Ki-67 and stem cell factor were analyzed by immunohistochemistry. RESULTS: GSE treatment proliferated and migrated human dermal papilla cells (hDPCs) more than treatment of 10 µM minoxidil. GSE significantly stimulated the expression of Ki-67 protein and the mRNA levels of hepatocyte growth factor and vascular endothelial growth factor in hDPCs. Topical application of 1,000 ppm GSE for 3 weeks promoted more significant hair growth on shaved C57BL/6 mice than did 5% minoxidil. The histological morphology of hair follicles demonstrated an active anagen phase with the induction of stem cell factor. GSE treatment significantly reduced the number of mast cells and the expression of transforming growth factor beta 1 in mouse skin tissues. CONCLUSIONS: These results demonstrated that GSE promotes hair growth in vitro and in vivo by regulating growth factors and the cellular response.
Assuntos
Derme/efeitos dos fármacos , Geranium , Cabelo/efeitos dos fármacos , Fator de Crescimento de Hepatócito/metabolismo , Extratos Vegetais/farmacologia , Fator de Crescimento Transformador beta1/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Movimento Celular , Proliferação de Células , Células Cultivadas , Derme/metabolismo , Cabelo/crescimento & desenvolvimento , Folículo Piloso/efeitos dos fármacos , Fator de Crescimento de Hepatócito/genética , Humanos , Antígeno Ki-67/metabolismo , Masculino , Mastócitos/metabolismo , Camundongos Endogâmicos C57BL , RNA Mensageiro/metabolismo , Fator de Células-Tronco/metabolismo , Fator A de Crescimento do Endotélio Vascular/genéticaRESUMO
Ophiopogon japonicus is known to have various pharmacological effects. The present study investigated the effects of an extract of fermented Ophiopogon japonicas (FEOJ) on thrombintreated vascular smooth muscle cells (VSMCs). FEOJ treatment inhibited the proliferation of VSMCs treated with thrombin as indicated by an MTT assay. These inhibitory effects were associated with decreased phosphorylation of AKT, reduced expression of cyclin D1 and increased expression of p27KIP1 in thrombininduced VSMCs. In addition, FEOJ treatment suppressed the thrombinstimulated migration of VSMCs as demonstrated by a woundhealing migration assay. Furthermore, zymographic analyses demonstrated that treatment of FEOJ with VSMCs suppressed the thrombininduced expression of matrix metalloproteinase (MMP)2, which was attributed to the reduction of nuclear factor (NF)κB binding activity. Collectively, these results demonstrated that FEOJ induced p27KIP1 expression, reduced cyclin D1 expression and AKT phosphorylation, and inhibited MMP2 expression mediated by downregulation of NFκB binding activity in thrombintreated VSMCs, which led to growth inhibition and repression of migration. These results supported the use of FEOJ for the prevention of vascular diseases and provided novel insight into the underlying mechanism of action.
