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1.
PLoS One ; 14(6): e0218571, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31247046

RESUMO

This paper reports an outdoor-use polymerase chain reaction (PCR) technology in which stability of resistance temperature detectors (RTDs) is remarkably improved. A thin-film RTD made of non-annealed Pt shows accuracy degradation because the resistance of the RTD tends to decrease during the PCR operation. Thus, the annealing process is applied to the Pt RTD to improve the stability, which is a prerequisite to the accurate measurement of the absolute temperature. Both heaters and the RTD are fabricated on a thin quartz substrate whose melting temperature is high enough for annealing. The performances in the PCR time and power consumption are enhanced by reducing the size of the heater chips with no degradation in the temperature uniformity. A spring-loaded electrode is employed to simplify the procedure of electrical connection to the thermal controller and loading/unloading of the PCR chip. The contact area of the electrical connection is so small that the conductive thermal resistance increases; thereby small heat dissipation can be exploited for low-power operation. The stability of the RTD is experimentally confirmed in terms of resistance variation over repeated PCR operations (four times). The least variation of 0.005%, which corresponds to a negligible temperature variation of 0.038 °C for the PCR, is achieved from the RTD annealed for 5 min at 450 °C. The gel-electrophoresis result indicates that the PCR performance of the proposed system using a film-type PCR chip is comparable to that of a conventional system using a vial tube despite its low power consumption.


Assuntos
Reação em Cadeia da Polimerase/instrumentação , Simulação por Computador , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Impedância Elétrica , Fontes de Energia Elétrica , Eletroforese , Desenho de Equipamento , Escherichia coli/genética , Humanos , Platina , Reação em Cadeia da Polimerase/estatística & dados numéricos , Temperatura
2.
Biomed Microdevices ; 20(1): 14, 2018 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-29376193

RESUMO

Polymerase chain reaction (PCR) has been widely used for major definite diagnostic tool, but very limited its place used only indoor such as hospital or diagnosis lab. For the rapid on-site detection of pathogen in an outdoor environment, a low-power cordless polymerase chain reaction (PCR) thermal cycler is crucial module. At this point of view, we proposed a low-power PCR thermal cycler that could be operated in an outdoor anywhere. The disposable PCR chip was made of a polymeric (PI/PET) film to reduce the thermal mass. A dual arrangement of the Pt heaters, which were positioned on the top and bottom of the PCR chip, improved the temperature uniformity. The temperature sensor, which was made of the same material as the heater, utilized the temperature dependence of the Pt resistor to ensure simple fabrication of the temperature sensor. Cooling the PCR chip using dual blower fans enabled thermal cycling to operate with a lower power than that of a Peltier element with a high power consumption. The PCR components were electrically connected to a control module that could be operated with a Li-ion battery (12 V), and the PCR conditions (temperature, time, cycle, etc.) were inputted on a touch screen. For 30 PCR cycles, the accumulated power consumption of heating and cooling was 7.3 Wh, which is easily available from a compact battery. Escherichia coli genomic DNA (510 bp) was amplified using the proposed PCR thermal cycler and the disposable PCR chip. A similar DNA amplification capability was confirmed using the proposed portable and low-power thermal cycler compared with a conventional thermal cycler.


Assuntos
Reação em Cadeia da Polimerase/instrumentação , Fontes de Energia Elétrica , Desenho de Equipamento , Escherichia coli/genética , Polietilenotereftalatos/química , Reação em Cadeia da Polimerase/métodos , Polímeros/química , Temperatura
3.
Brain Stimul ; 8(5): 914-25, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26209594

RESUMO

BACKGROUND: Although computational studies of electrical brain stimulation (EBS) have received attention as a cost-effective tool, few studies have validated the technique, particularly in invasive cortical stimulation. OBJECTIVE: In order to validate such studies, we used EBS to compare electric potential distributions generated by both numerical simulations and empirical measurements in three phantom head models (one-/three-layered spherical heads and MRI-based head). METHODS: We constructed spherical phantom heads that consisted of one or three layers, and an anatomical, MRI-based phantom that consisted of three layers and represented the brain or brain/skull/scalp in order to perform both numerical simulations using the finite element method (FEM) and experimental measurements. Two stimulation electrodes (cathode and anode) were implanted in the phantoms to inject regulated input voltage, and the electric potential distributions induced were measured at various points located either on the surface or deep within the phantoms. RESULTS: We observed that both the electric potential distributions from the numerical simulations and experiments behaved similarly and resulted in average relative differences of 5.4% (spherical phantom) and 10.3% (MRI-based phantom). CONCLUSIONS: This study demonstrated that numerical simulation is reasonably consistent with actual experimental measurements; thus, because of its cost-effectiveness, EBS computational studies may be an attractive approach for necessary intensive/extensive studies.


Assuntos
Encéfalo/fisiologia , Simulação por Computador , Estimulação Elétrica , Humanos , Modelos Neurológicos , Imagens de Fantasmas , Técnicas Estereotáxicas
4.
J Endourol ; 29(8): 933-8, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25811682

RESUMO

PURPOSE: This article aims to describe the design of an anti-reflux ureteral stent with a polymeric flap valve and the fabrication methods using three-dimensional (3D) printing. The stent effectively prevents backward flow with a negligible reduction in forward flow. Fabrication of miniaturized valves was easy with high precision and rapid prototyping. MATERIALS AND METHODS: The proposed stent comprised a 7F Double-J (DJ) stent and a polymeric flap valve. The valve was made of Tango Plus FLX980 and was fabricated using a 3D printer. Two types of stent were prepared for in vitro tests: DJ stents with (1) an uncoated valve (UCV) stent and (2) a parylene C coated valve (PCV) stent for enhanced biocompatibility. The flow characteristics of each stent were evaluated considering flow direction, parylene coating, and stent side holes, and were compared to the intact DJ stent. RESULTS: The forward flow rate for the distal portion of the UCV and PCV stents was 9.8 mL/min and 7.8 mL/min at applied pressure of 15 cm H2O (normal anterograde pressure in patients with stents), respectively. Backward flow rate for the distal portion of the UCV and PCV stents was decreased by 28 times and 8 times at applied pressure of 50 cm H2O (maximum bladder pressure), respectively, compared with the distal portion of the intact DJ stent. Forward flow rates of whole stents were 22.2 mL/min (UCV stent) and 20.0 mL/min (PCV stent) at applied pressure of 15 cm H2O, and backward flow rates of whole UCV and PCV stents were decreased by 8.3 times and 4.0 times at applied pressure of 50 cm H2O, respectively, compared with the intact DJ stent. CONCLUSIONS: The anti-reflux ureteral stent was successfully designed and fabricated using a 3D printer. In vitro studies showed that the stent effectively prevented backward flow while minimizing reduction in forward flow.


Assuntos
Desenho de Equipamento/métodos , Impressão Tridimensional , Stents , Refluxo Vesicoureteral/cirurgia , Materiais Biocompatíveis , Humanos , Técnicas In Vitro , Polímeros , Xilenos
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