Effect of developmental temperatures on Aphidius colemani host-foraging behavior at high temperature.

Temperatures experienced by insects during their adult life often differ from developmental temperatures. Yet, developmental thermal acclimation can play an important role in shaping physiological, morphological, and behavioral traits at the adult stage. We explored how three rearing temperatures (10, 20, and 28 °C) affected host-foraging behaviors and associated traits under warm conditions in the parasitoid Aphidius colemani, a key model in behavioral ecology and an important natural enemy of aphids. Developmental time was longer at lower temperatures, resulting in bigger emerging parasitoids, with higher egg-loads. Parasitism rates, emergence rates, and parasitoid survival (once placed at high temperature) were the highest for parasitoids developed at 20 °C. When exposed to 28 °C, the expression of all behavioral items (time spent walking searching for hosts, number of antennal and ovipositor contacts with hosts) was higher for parasitoids reared at 20 °C, followed by those reared at 10 °C, then those reared at 28 °C. Finally, we showed that parasitoid residence time on aphid patches was determined by both developmental temperatures and the number of host encounter without oviposition, representative of the resource quality. We revealed that developing at 28 °C did not lead to increased adult performance at this temperature, probably because of complex interactions and trade-offs between developmental costs at high temperature and optimal foraging behaviors (e.g., parasitoid size and host-handling capacities). Our results strengthen the idea that thermal developmental plasticity may play an important role in insect behavioral responses to varying temperatures, and is important to consider in the context of climate change.

Developmental Temperature Affects Life-History Traits and Heat Tolerance in the Aphid Parasitoid Aphidius colemani.

Developmental temperature plays important roles in the expression of insect traits through thermal developmental plasticity. We exposed the aphid parasitoid Aphidius colemani to different temperature regimes (10, 20, or 28 °C) throughout larval development and studied the expression of morphological and physiological traits indicator of fitness and heat tolerance in the adult. We showed that the mass decreased and the surface to volume ratio of parasitoids increased with the development temperature. Water content was not affected by rearing temperature, but parasitoids accumulated more lipids when reared at 20 °C. Egg content was not affected by developmental temperature, but adult survival was better for parasitoids reared at 20 °C. Finally, parasitoids developed at 20 °C showed the highest heat stupor threshold, whereas parasitoids developed at 28 °C showed the highest heat coma threshold (better heat tolerance CTmax1 and CTmax2, respectively), therefore only partly supporting the beneficial acclimation hypothesis. From a fundamental point of view, our study highlights the role of thermal plasticity (adaptive or not) on the expression of different life history traits in insects and the possible correlations that exist between these traits. From an applied perspective, these results are important in the context of biological control through mass release techniques of parasitoids in hot environments.