RESUMO
Systematics of Chytridiales has been deeply influenced by analyses of molecular loci and zoospore ultrastructure. Even though the Chytridiales is the largest order within Chytridiomycota, Brazilian isolates of this clade have been poorly integrated. Here, we isolated seven species and documented their morphology, including zoospore ultrastructure for Siphonaria aurea, and phylogenetic positions for all based on analyses of nuc 18S and 28S rDNA. Phylogenetic results support the placement of these species in Chytriomycetaceae and Chytridiaceae, with two new species described, Rhizidium crepaturum and Siphonaria aurea, and Rodmanochytrium sphaericum recorded for the first time from Brazil.
Assuntos
Quitridiomicetos/classificação , Quitridiomicetos/genética , DNA Fúngico/genética , Microscopia Eletrônica de Transmissão , Filogenia , Brasil , Quitridiomicetos/citologia , Quitridiomicetos/ultraestrutura , DNA Ribossômico/genética , RNA Ribossômico 28S/genética , Análise de Sequência de DNA , Esporos Fúngicos/genéticaRESUMO
Six Nowakowskiella species from Brazil were identified and purified on corn meal agar (CMA) plus glucose and Peptonized Milk-Tryptone-Glucose (PmTG) media and placed into a phylogenetic framework for the genus. New sequence data are presented for four species: N. elongata, N. multispora, and N. ramosa and the new species N. crenulata. Our maximum likelihood and Bayesian analyses of combined 18S, 5.8S, and 28S subunits of nuc rDNA showed that Nowakowskiella is not a monophyletic clade because of the position of Nowakowskiella elongata, which is more related to Cladochytrium. We reclassify N. elongata as the type of a new genus, Karlingiella.
