RESUMO
Using five bioinformatics analysis software, we identified Golgi protein 73 (GP73) as a putative target of microRNA-27b (miR-27b), which is closely related to various biological processes or diseases such as bone metabolism disease, adipose cell and muscle cell development, pulmonary hypertension, cervical cancer, and breast cancer. However, the clinical significance of miR-27b in hepatocellular carcinoma (HCC) is still unclear. The differential expression of miR-27b in HCC and adjacent normal liver tissues was measured by quantitative reverse transcription PCR. Our results showed that the expression of miR-27b in tumor tissues is lower than that in adjacent nontumor tissues. The expression of miR-27b was significantly lower in HCC tissues with high expression of GP73, when compared with adjacent nontumor tissues. Moreover, down-regulated expression of miR-27b was closely correlated with serum GP73, tumor-node-metastasis stage, tumor size, and portal vein thrombosis. GP73 mRNA might be a target of miR-27b. The 5-year overall survival rate of the low miR-27b expression group was significantly lower than that of the high miR-27b expression group. Moreover, multivariate analysis of prognostic factors, with a Cox proportional hazards model, showed that low miR-27b expression was a significant and independent predictor of poor survival in HCC. Hence, the abnormal expression of miR-27b might be related to the occurrence and development of tumors. Similarly, a study in the Cancer Genome Atlas database demonstrated that the expression of miR-27b in 50 normal individuals was 1.6 times higher than that of 372 patients with liver cancer. The overall survival rate of the low GP73 expression group (275 liver cancer patients) was significantly longer than that of the high GP73 expression group (90 normal individuals). MiR-27b suppresses the expression of GP73 and is therefore a potential prognostic biomarker and therapy target in HCC.
Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma Hepatocelular/patologia , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/patologia , Proteínas de Membrana/metabolismo , MicroRNAs/genética , Biomarcadores Tumorais/genética , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , Proliferação de Células , Progressão da Doença , Feminino , Seguimentos , Células Hep G2 , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Masculino , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Prognóstico , Taxa de SobrevidaRESUMO
Personal identification is an important work in forensic investigation included sex discrimination, age and stature estimation. Human identification depended on radiological image technique analysis is a practice and proper method in forensic science field. This paper intended to understand the advantage and defect by reviewed the employing of forensic radiology in forensic science field broadly and provide a reference to perfect the application of forensic radiology in forensic science field.
Assuntos
Humanos , Determinação da Idade pelo Esqueleto , Estatura , Osso e Ossos/diagnóstico por imagem , Diagnóstico por Imagem/métodos , Antropologia Forense/métodos , Consolidação da Fratura/fisiologia , Fraturas Ósseas/patologia , Processamento de Imagem Assistida por Computador/métodos , Imageamento Tridimensional , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodosRESUMO
OBJECTIVE@#To explore the species identification methods of bone remain.@*METHODS@#The compare between human bone and common animals bones was made with microscopic comparison methods. The sample was consisted of 77 slides including human, cattle, house, pig, goat, sheep, monkey, cat, rabbit, chicken, duck, pigeon, tortoise, frog, fish.@*RESULTS@#The differences of the histological bone species were as follow: the thick of the outer and the inner circumferential lamellae, and the shape of interstitial lamellae, and the size of Haversian system, and the number of the circumferential lamellae, and the number of the lacunae of inner Haversian system.@*CONCLUSION@#The histological characters of the bones can be used for species identification.
