A correlation study of the expression of HA-CD44st and HER-2 in breast cancer.

BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND METHODS: MCF-7 cells transfected with the eukaryotic expression vector pcDNA3.1-CD44st (MCF/CD44st) were used to examine the effect of the activation of the HA-CD44st-transforming growth factor ß (TGFß)-phosphatidylinositol-3-kinase (PI3K) signaling pathway on the invasive ability of MCF-7 cells. The expression of proteins related to this signaling pathway was assessed by flow cytometry, reverse transcription-polymerase chain reaction, and Western blotting, and the role of AP-1 in the pathway was investigated by electrophoretic mobility shift assay. The effect of pathway activation on the invasion of MCF-7 cells was assessed by Transwell assay, and CD44 expression in breast cancer tissue was detected by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of CD44st and HER-2 in breast cancer tissue and their correlation was investigated. RESULTS: HA significantly upregulated HER-2 and TGFß in MCF-7/CD44st cells, increased p-AKT expression and AP-1 activity, and promoted the invasive ability of tumor cells. CD44st mRNA expression had significant difference between breast cancer tissues and adjacent normal tissues (P < 0.05), and high expression of CD44st mRNA was closely correlated with HER-2 expression in breast cancer tissues. CONCLUSION: Binding of HA to the CD44st receptor may regulate the invasiveness of MCF-7 cells through the CD44st/TGFß/PI3K/AP-1 signaling pathway with increased expression of TGFß and HER-2. The expression of CD44st mRNA is correlated with HER-2 expression in postoperative breast cancer patients.

[Effect of AAVC-I on the migration of human umbilical vein endothelial cells].

OBJECTIVE: To investigate the effect of component I from agkistrodon acutus venomon (AAVC-I) the migration of human umbilical vein endothelial cells (HUVECs), and to elucidate the possible anti-angiogenic mechanism of AAVC-I. METHODS: The effect of AAVC-I on the migration of HUVECs which was cultivated in vitro and treated with AAVC-1 at four concentrations: 0, 20, 40, 80 microg/ml, was observed by methods of scratch wound-healing and Transwell assay. The expression level of mRNA and protein of P-selectin and intercellular cell adhension molecule-I (ICAM-1) were examined by RT-PCR and Western blot assay. RESULTS: Compared with the blank group, the migration ability of HUVECs in each AAVE-I treated group was reduced in a dose-dependent manner, and the expression level of the mRNA and protein of P-selectin and ICAM-1 were decreased. CONCLUSION: AAVC-I inhibits the migration of endothelial cell, which is acted by down-regulation of the expression content of mRNA and protein of P-selectin and ICAM-1.

Clinical significance of nuchal ligament calcification and the discussion on biomechanics / 中国骨伤

<p><b>OBJECTIVE</b>To improve the cognition about X-ray characteristics of nuchal ligament calcification, and discuss biomechanical properties.</p><p><b>METHODS</b>From September 2008 to August 2009, the X-rays of 163 cases showed nuchal ligament calcification, which datas were analyzed. There were 91 males and 72 females, the age from 38 to 68 with an average of 53.6 years. And the number of calcification segment, related vertebral level, and vertebral hyperosteogeny, intervertebral space or intervertebral foramen stenosis, anterior or posterior longitudinal ligament calcification, cervical instability in the relative segment were observed.</p><p><b>RESULTS</b>One hundred forty-one patients occurred degeneration in the relative segment. The characteristics of X-ray: the multiple nuchal ligament calcification existed single segment and the C5,6 and C4,5 were readily occurred, which multiple related vertebral sclerotin, intervertebral space or intervertebral foramen, anterior or posterior longitudinal ligament occurred pathological changes, moreover, it was main segment of pathological changes. The X-rays of 93 cases showed vertebral hyperosteogeny 72 intervertebral space or intervertebral foramen stenosis, 49 anterior or posterior longitudinal ligament calcification, 66 cervical instability in the relative segment. The percentage was respectively 65.96%, 51.06%, 34.75%, 46.81%.</p><p><b>CONCLUSION</b>Cervical X-ray examination could discover characteristics of nuchal ligament calcification in the relative segment, also is helpful to diagnosis of cervical spondylosis.</p>

Correlation between body mass index, waist circumference and blood pressure in rural residents from west part of Liaoning Province / 中华心血管病杂志

<p><b>OBJECTIVE</b>This study observed the association between body mass index (BMI), waist circumference (WC) and blood pressure level in rural residents from west Liaoning province.</p><p><b>METHODS</b>This epidemiological study using stratified cluster random sampling was conducted from 2004 to 2006 in Fuxin County, Liaoning Province, 43,692 rural residents (21,680 males) aged 35-74 years old [(49. 8 +/-10.2) years] were surveyed. Database was established with the help of Epidata 3.1 software.</p><p><b>RESULTS</b>In total 43,692 persons were surveyed, including 21,680 male (49.6%) and 22,012 female (50.4%). The average BMI and WC was (23.31 +/- 3.08) kg/m2 and (80.87 +/- 9.0) cm, respectively. No matter male or female, SBP started from 20 kg/m2, increased with the increase of BMI; DBP increased gradually with the increase of BMI; the prevalence of hypertension were significant differences among different BMI groups (P < 0.001). Multiple logistic regression show that in male, using the group with BMI, <18 kg/m2 as control, 28-30 kg/m2 group OR and 95% CI was 6.285 (4.612-8.566); in female, when BMI >22 kg/ m2 OR increased with the increasing of BMI. In male and female, both SBP and DBP, also the prevalence rate of hypertension increased gradually with the increase of WC (P < 0.001). No matter in male or female, when BMI <24 kg/m2, and WC male <85 cm, female WC <80 cm, the average blood pressure levels and prevalence of hypertension are the lowest; after adjusting for age and other risk factors, the prevalence rate of overweight and obesity for male with hypertension OR are 1.704 (1.592-1.825) and 3.710 (3.148 -4.371) , respectively, for female is 1.527 (1.428-1.632) and 3.014 (2.668-3.405), respectively. When the WC is higher than the standard, male and female hypertension risk OR and 95% CI are 1.231 (1.153-1.314) and 1.353 (1.269-1.442), respectively.</p><p><b>CONCLUSION</b>Both BMI and WC are risk factors of hypertension.</p>

Prevalence of metabolic syndrome among rural population with hypertension in Fuxin of Liaoning Province / 中华内分泌代谢杂志

Through cluster multistage sampling,a resident group of 6 412 subjects with hypertension in the rural area of Liaoning province were recruited.According to IDF and NCEP-ATPm criteria the age-standardized prevalences of metabolic syndrome were 36.2%and 25.9%respectively.The prevalence of metabolic syndrome decreased with advancing age,but increased with rising of blood pressure.