RESUMO
OBJECTIVE: To investigate the expression and clinical significance of Lin28 B in placenta of severe preeclampsia(SPE).METHODS: Forty SPE patients were admitted to Shengjing Hospital of China Medical University from August 2017 to August 2018,including 20 patients with early-onset severe preeclampsia(ESPE)and 20 patients with late onset severe preeclampsia(LSPE).Another 40 healthy pregnant women who had termination of pregnancy in late pregnancy due to various reasons were selected as the control group,including 20 cases in early-onset control group(N1) and 20 cases in late-onset control group(N2). RT-qPCR analysis,Western Blot analysis and immunohistochemistry were used to detect the Lin28 B expression levels in placenta of each group.RESULTS: The expression of Lin28 B in placenta was significantly lower in ESPE group than in N1 group(P0.05).The expression of Lin28 B in placenta of ESPE group was lower than that in LSPE group(P<0.05).CONCLUSION: The expression of Lin28 B in placenta of SPE patients is decreased,and the ESPE group was significantly lower than that in LSPE group,suggesting that Lin28 B may be associated with the pathogenesis and development of SPE.
