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1.
Journal of Forensic Medicine ; (6): 581-586, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-692364

RESUMO

Objective To observe the protein expression patterns of matrix metalloproteinase (MMP)-2 and MMP-9 in the liver tissue of liver contusion rats at different time after impact. Methods Fifty healthy adult male SD rats were randomly and evenly divided into control group and experimental groups (1 h, 3 h, 6 h, 12 h, 18 h, 24 h, 3 d, 5 d, 7 d after liver contusion). A rat liver contusion model was established by a free-falling device. The rats were killed at corresponding time, and the contused hepatic lobes were extracted. The protein expressions of MMP-2 and MMP-9 in contused liver tissue of the rats in each group were observed by immunohistochemical staining (SP method) and Western blotting. Results After the liver contusion, the expression of positive cell and the protein semiquantitative result showed that the protein expression of MMP-2 enhanced at 6 h and peaked at 24 h, then decreased gradually at 3-5 d, and returned to normal levels at 7 d. The difference of expression between group and its previous adjacent group after 6 h (except 18 h) had statistical significance (P<0.05). The protein expression of MMP-9 rose obviously at 1 h after liver contusion and peaked at 18 h, then decreased gradually at 3-7 d which still higher than control group. The expression difference between group and its previous adjacent group (except 12 h and 24 h) had statistical significance (P<0.05). Conclusion The protein expressions of MMP-2 and MMP-9 in contused liver tissue after impact show good time-dependent patterns, which may provide important reference indicators for the time estimation of liver contusion.

2.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-273771

RESUMO

<p><b>OBJECTIVE</b>To compared the efficacy of drug-coated balloon and common balloon for treatment of superficial femoral artery and popliteal artery occlusive disease.</p><p><b>METHODS</b>Forty-six patients were admitted for ipsilateral single or multiple superficial femoral artery and/or popliteal artery lesions (between 3 and 15 cm stenosis or occlusion), Rutherford grades 2 to 5, with or without other accompanying diseases in the Department of Interventional Vascular Therapy of the First Hospital of Nanjing between September, 2015 and December, 2016. The patients were randomly assigned into drug-coated balloon (DCB) group (n=23) and common balloon (CB) group (n=23). None of the patients had stent restenosis, aneurysms, acute thrombosis, pregnancy, life expectancy less than 1 year, or below-the-knee artery occlusion. The late lumen loss (LLL), improvement of the ankle brachial index (ABI), improvement of Rutherford grade, incidence of restenosis, thrombosis rate and amputation rate were compared between the two groups at 6 months after treatment.</p><p><b>RESULTS</b>The two groups of patients were comparable for general conditions, risk factors, and characteristics of the compromised vessels (P>0.05). Six months after treatment, the patients in DCB group showed significantly smaller LLL, more obvious improvement of the ABI and Rutherford grade, and lower restenosis rate and thrombosis rate than those in CB group (P<0.05). The amputation rates were similar between the two groups (P>0.05).</p><p><b>CONCLUSIONS</b>DCB shows obvious advantages over common balloon for treatment of superficial artery and popliteal artery arteriosclerosis obliterans in that it more effectively reduces LLL, restenosis rate and thrombosis rate and improves the ABI and Rutherford grade at 6 months after the treatment.</p>

3.
Vascular ; 22(6): 432-8, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24459129

RESUMO

This study evaluates the efficacy of dual Willis covered stents for the treatment of large fusiform carotid aneurysms in a canine model. Carotid fusiform aneurysms >10 mm long were surgically created in 10 dogs and were then repaired using either single or dual covered stents. Clinical results were assessed by scheduled angiography and histological features by light and electron microscopy. Angiography immediately post-op and 6 months after surgery revealed aneurysm isolation rates of 60 and 20% for the single stent technique and 60 and 100% for the dual stent technique, respectively. The rate of complete obliteration of the aneurysm sac differed significantly between treatments (P = 0.048). The dual stent technique also resulted in greater endothelialization. For large carotid fusiform aneurysms in a canine model, endovascular repair using dual Willis covered stents is technically feasible and more effectively obliterates the aneurysm sac than the use of a single stent.


Assuntos
Aneurisma/terapia , Doenças das Artérias Carótidas/terapia , Stents , Animais , Modelos Animais de Doenças , Cães , Procedimentos Endovasculares , Desenho de Prótese , Implantação de Prótese
4.
Vascular ; 21(4): 215-9, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23518853

RESUMO

Endovenous radiofrequency ablation (RFA) has been introduced as a less invasive alternative to traditional surgery for varicose veins, but vein thrombosis after RFA remains a concern. This study investigated the influence of endovenous RFA on coagulable states of canine blood, and changes in the vein wall and lumen. Eight canines underwent RFA of the iliac-femoral vein. Levels of plasma granule membrane protein-140, tissue plasminogen activator and plasminogen activator inhibitor-1 increased at different time points after RFA, which were monitored by enzyme-linked immunosorbent assay. Venography after ablation showed that there was left iliac-femoral vein occlusion in three canines and vein stenosis in the remaining five. Histological examination revealed intimal injury and inflammatory cell infiltration of the vessel wall. Our findings suggest that RFA is an effective microinvasive technique, which can result in a hypercoagulable state, with venous thrombosis and stenosis.


Assuntos
Veia Safena , Varizes , Animais , Ablação por Cateter , Cães , Veia Femoral/cirurgia , Terapia a Laser , Veia Safena/cirurgia , Resultado do Tratamento , Varizes/cirurgia , Trombose Venosa/cirurgia
5.
Can J Microbiol ; 57(6): 514-24, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21635219

RESUMO

The Rhizobium sp. strain PR389 was isolated from the root nodules of Pueraria lobata (Willd.) Ohwi, which grows in acidic (pH 4.6) yellow soil of the Jinyun Mountains of Beibei, Chongqing, China. While rhizobia generally have a pH range of 6.5-7.5 for optimum growth, strain PR389 grew in a liquid yeast extract - mannitol agar medium at pH 4.6, as well as in a pH 4.1 soil suspension, suggesting acid tolerance in this specific strain of rhizobium . However, at pH 4.6, the lag phase before vigorous growth was 40 h compared with 4 h under neutral conditions (pH 7.0). For PR389, the generation time after the lag phase remained the same at different pH levels despite the different durations of the lag phase. Except in the pH 4.4 treatment, the pH of the culturing media increased from 4.6, 4.8, 5.0, and 5.5 to neutral and slightly alkaline after 70 h of culture. Chloramphenicol was added to determine if protein production was involved in the increasing pH process. Chloramphenicol significantly inhibited PR389 growth under acid stress but had little effect under neutral conditions. Proton flux measured during a short acid shock (pH 3.8) revealed that this strain has an intrinsic ability to prevent H(+) from entering cells when compared with acid-sensitive rhizobia. We propose that the mechanism for acid tolerance in PR389 involves both intracellular and extracellular processes. When the extracellular pH is lower than pH 4.4, the cell membrane blocks hydrogen from entering the cell. When the pH exceeds 4.4, the rhizobium strain has the ability to raise the extracellular pH, thereby, potentially decreasing the toxicity of aluminum in acid soil.


Assuntos
Ácidos/farmacologia , Pueraria/microbiologia , Rhizobium/efeitos dos fármacos , Microbiologia do Solo , China , Cloranfenicol/química , Meios de Cultura/química , Concentração de Íons de Hidrogênio , Rhizobium/crescimento & desenvolvimento , Rhizobium/metabolismo , Nódulos Radiculares de Plantas/microbiologia , Estresse Fisiológico
6.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-266282

RESUMO

<p><b>OBJECTIVE</b>To explore the associations of triple-phase enhancement multi-slice CT scan with histological differentiation and lymphovascular invasion in advanced gastric cancer.</p><p><b>METHODS</b>Patients with gastric cancer undergoing surgical resection between January 2006 and December 2009 were included, all of whom underwent routine and two-phase contrast enhancement multi-slice CT examinations of the upper abdomen before surgery. Patients with incomplete data were excluded. Postoperative specimens were used for evaluation of histological differentiation, microscopic lymphovascular invasion, and CD34 and D2-40 expression. Associations between contrast enhancement ratio (CER) of triple-phase multi-slice CT scan and differentiation as well as lymphovascular invasion were analyzed.</p><p><b>RESULTS</b>A total of 64 patients were included. There were significant associations between CER and tumor differentiation (P<0.05). Compared to those without microvascular invasion, CER of the arterial phase in gastric cancer with microvascular invasion was significantly higher (0.61±0.28 vs. 0.46±0.14, P<0.05), and that of the arterial-parenchymal phase was significantly lower(1.81±0.39 vs. 2.28±0.80, P<0.05). However, gastric cancers with lymphatic invasion were associated with significantly higher CER of the parenchymal phase than their counterparts(1.25±0.57 vs. 1.00±0.35, P<0.05).</p><p><b>CONCLUSION</b>CER of triple-phase multi-slice CT scan can reveal the differentiation of gastric cancer and is associated with microvascular and lymphatic invasion.</p>


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Metástase Linfática , Estadiamento de Neoplasias , Neoplasias Gástricas , Diagnóstico por Imagem , Patologia , Tomografia Computadorizada por Raios X , Métodos
7.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-60038

RESUMO

OBJECTIVE: To evaluate the value of early identification and endovascular treatment of iliac vein compression syndrome (IVCS), with or without deep vein thrombosis (DVT). MATERIALS AND METHODS: Three groups of patients, IVCS without DVT (group 1, n = 39), IVCS with fresh thrombosis (group 2, n = 52) and IVCS with non-fresh thrombosis (group 3, n = 34) were detected by Doppler ultrasonography, magnetic resonance venography, computed tomography or venography. The fresh venous thrombosis were treated by aspiration and thrombectomy, whereas the iliac vein compression per se were treated with a self-expandable stent. In cases with fresh thrombus, the inferior vena cava filter was inserted before the thrombosis suction, mechanical thrombus ablation, percutaneous transluminal angioplasty, stenting or transcatheter thrombolysis. RESULTS: Stenting was performed in 111 patients (38 of 39 group 1 patients and 73 of 86 group 2 or 3 patients). The stenting was tried in one of group 1 and in three of group 2 or 3 patients only to fail. The initial patency rates were 95% (group 1), 89% (group 2) and 65% (group 3), respectively and were significantly different (p = 0.001). Further, the six month patency rates were 93% (group 1), 83% (group 2) and 50% (group 3), respectively, and were similarly significantly different (p = 0.001). Both the initial and six month patency rates in the IVCS patients (without thrombosis or with fresh thrombosis), were significantly greater than the patency rates of IVCS patients with non-fresh thrombosis. CONCLUSION: From the cases examined, the study suggests that endovascular treatment of IVCS, with or without thrombosis, is effective.


Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Angioplastia com Balão , Constrição Patológica/terapia , Diagnóstico por Imagem , Veia Ilíaca/patologia , Doenças Vasculares Periféricas/complicações , Estudos Retrospectivos , Stents , Trombectomia , Grau de Desobstrução Vascular , Filtros de Veia Cava , Trombose Venosa/complicações
8.
Chinese Medical Journal ; (24): 2487-2491, 2008.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-265909

RESUMO

<p><b>BACKGROUND</b>This study aimed to investigate multi-slice CT contrast-enhanced presentation of gastric cancer and its correlation with histo-differentiation and p53 and P-glycoprotein (P-gp) expression.</p><p><b>METHODS</b>Sixty-six patients with gastric cancer in the present study underwent a multi-slice CT preoperative routine and dual-phase contrast-enhanced examination of the upper abdomen; postoperative specimens were used to determine histo-differentiation and the expression of p53 and P-gp. The correlation of multi-slice CT contrast-enhanced presentation with histo-differentiation and expression of p53 and P-gp was analyzed.</p><p><b>RESULTS</b>The dual-phase contrast-enhanced ratio (CER) was not correlated with the histo-differentiation of gastric cancer (P > 0.05). Positive expression of p53 and P-gp was significantly higher in the cases of layered or heterogeneous enhancement than in the cases of homogenous enhancement (P < 0.05). Positive expression of p53 was also correlated with the arterial phase CER, tumor size and lymph node metastasis (P < 0.05), but not with infiltration thickness of the gastric wall, nor was it correlated with the portal phase CER (P > 0.05). Positive expression of P-gp was only correlated with the portal phase CER (P = 0.005).</p><p><b>CONCLUSIONS</b>Differently enhanced pattern and CER of the arterial and portal phase in gastric cancer correlate with its different histo-differentiation and expression of p53 and P-gp respectively. In addition, tumor size and lymph node metastasis of gastric cancer relate to the expression of p53.</p>


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Metabolismo , Imuno-Histoquímica , Neoplasias Gástricas , Diagnóstico por Imagem , Metabolismo , Patologia , Tomografia Computadorizada por Raios X , Métodos , Proteína Supressora de Tumor p53 , Metabolismo
9.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-680180

RESUMO

Objective To assess the clinical values of MR angiography(MRA)in the detection of deep vein thrombosis of the lower limbs.Methods Two-dimensional time of flight(2D TOF)MRA was performed in thirty patients who were suspected of having deep vein thrombosis in the lower limbs.The findings of MRA were compared to that of digital subtraction angiography(DSA).Results twenty-five cases showed deep vein thrombosis in the lower limbs,the MRA findings included venous filling defect (14 cases),occlusions and interruptions of veins(8 cases),venous recanalizations(3 cases),collateral veins(25 cases).Taking the results of DSA as a golden standard,MRA detected all of the affected cases with only one case as the false positive.Conclusion 2D TOF MRA is a method of choice in the diagnosis of deep vein thrombosis of the lower limbs.

10.
Microbiology ; (12)1992.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-684659

RESUMO

Inoculant the examine-needed germ on a flat panel of synthetic medium, and placed paper slices, which had being saturated by different kinds of sugar, on the medium to carry out the experiment of microorganic auxanography. Its result was compared with that of the traditional method, the sugar-grain method. The comparison showed that the former was better than the latter for its more definite result and more convenient operation.

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