RESUMO
The immunocytes microglia in the central nervous system (CNS) were reported to play a crucial role in neurodegeneration. As a member of P2 receptors family, purinoceptor P2Y6 has attracted much attention recently. Previous studies showed that purinoceptor P2Y6 mainly contributed to microglia activation and their later phagocytosis in CNS, while in immune system, it participated in the secretion of interleukin (IL)-8 from monocytes and macrocytes. So there raises a question: whether purinoceptor P2Y6 also takes part in neuroinflammation? Thus, this review mainly concerns about the properties and roles of purinoceptor P2Y6, including (1) structure of purinoceptor P2Y6; (2) distribution and properties of purinoceptor P2Y6; (3) relationships between purinoceptor P2Y6 and microglia; (4) relationships between purinoceptor P2Y6 and immunoinflammation. Itos proposed that purinoceptor P2Y6 may play a role in neuroinflammation in CNS, although further research is still required.
Assuntos
Animais , Humanos , Inflamação , Alergia e Imunologia , Metabolismo , Microglia , Metabolismo , Monócitos , Metabolismo , Fagocitose , Fisiologia , Receptores Purinérgicos P2 , Química , Genética , MetabolismoRESUMO
We conducted a case-control study to determine the prevalence of the LRRK2 Gly2385Arg variant in patients with Parkinson's disease in Han population in mainland China. Heterozygous LRRK2 Gly2385Arg variant was identified in 14 of 235 patients with Parkinson's disease (5.69%), but not in 214 unrelated healthy controls. Multivariate analysis indicated the frequency of Gly2385Arg variant in the female patients with early age at onset is higher than their male counterparts. The founder haplotype analysis showed the variant carriers shared the same founder. Clinically, the LRRK2 Gly2385Arg carriers presented with classical Parkinson's disease symptoms. Our study indicates that the LRRK2 Gly2385Arg variant is a potential ethnic-specific genetic risk factor of Parkinson's disease within Chinese Han ethnicity.
Assuntos
Doença de Parkinson/epidemiologia , Doença de Parkinson/genética , Proteínas Serina-Treonina Quinases/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Substituição de Aminoácidos , Antiparkinsonianos/uso terapêutico , Estudos de Casos e Controles , China/epidemiologia , DNA/genética , Feminino , Efeito Fundador , Genótipo , Haplótipos , Humanos , Serina-Treonina Proteína Quinase-2 com Repetições Ricas em Leucina , Levodopa/uso terapêutico , Masculino , Pessoa de Meia-Idade , Mutação/genética , Doença de Parkinson/fisiopatologia , Fenótipo , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Objective To detect pollution status of non_01 Vibrio cholerae in Haihe river by Vibrio cholerae outer membrane protein gene (ompw) PCR technology. Methods A pair of Vibrio cholera ompw primers was synthetized to detect non_01 Vibrio cholerae in 102 water samples collected from 5 sampling sites in Haihe river by ompw PCR approach and the results were compared with that of the routine bacterial separation culture method. When amplified by PCR, isolated non_01 Vibrio cholerae strain Vbo35 was used as positive reference, Shigella, E.coli and Salmonella strains were used as negative reference in the study. Results The total positive rate of Vibrio cholerae in 102 water samples was 52% and the amplified products were 588 bp. The detection results of the PCR approach were 100% in accord with that of the routine bacterial separation culture method. Conclusion The results indicated that ompw PCR technology was a highly sensitive and specific method to determination of non_01 Vibrio cholerae in Haihe river water system, it shortened the determination time 4～5 times, and could provided a useful determination method for non_01 Vibrio cholerae.
