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1.
Zhongguo Zhong Yao Za Zhi ; 47(5): 1253-1261, 2022 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-35343152

RESUMO

This study aims to explore the effects of chemical ingredient groups B and C in Kansui Radix stir-fried with vinegar on the diversity of gut microbiota in the rat model of malignant ascites, identify the key differential microbial taxa, and reveal the biological mechanism of water-expelling effect of the two chemical ingredient groups. The rat model of malignant ascites induced by Walker-256 cells was established, and phenolphthalein was used as the positive drug. The rats were orally administrated with corresponding agents for consecutive 7 days. On day 6, fresh feces samples were collected from the rats, and 16 S rDNA high-throughput sequencing and GC-MS were employed to determine the composition of gut microbiota and the content of short-chain fatty acids, respectively. On day 7, serum and intestinal tissue samples were collected for the determination of related indicators. Compared with the control group, the model group showed decreased feces volume and urine volume(P<0.01), increased volume of ascites and levels of Na~+, K~+, and Cl~- in urine(P<0.01), down-regulated mRNA and protein levels of intestinal AQP8(P<0.01), lowered abundance of beneficial Lactobacillus(P<0.01) while risen abundance of potential pathogenic Lachnospiraceae and Anaeroplasma(P<0.01), and reduced content of short-chain fatty acids(P<0.01). Compared with the model group, administration with chemical ingredient groups B and C alleviated all the above indicators(P<0.01). In conclusion, chemical ingredient groups B and C in Kansui Radix stir-fried with vinegar could alleviate the disordered gut microbiota in rats with malignant ascites to expel water through increasing the abundance of beneficial Lactobacillus and reducing the abundance of harmful Lachnospiraceae and Anaeroplasma. This study can provide a reference for the reasonable clinical application of Kansui Radix stir-fried with vinegar.


Assuntos
Euphorbia , Microbioma Gastrointestinal , Ácido Acético/química , Animais , Ascite/tratamento farmacológico , Euphorbia/química , Raízes de Plantas/química , Ratos
2.
Zhongguo Zhong Yao Za Zhi ; 47(6): 1558-1566, 2022 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-35347953

RESUMO

Utilizing metabolomics technology, this study explored the change of fecal endogenous metabolites in Walker-256 rats with malignant ascites after the administration with Kansui Radix(KR) stir-fried with vinegar(VKR), sought the potential biomarkers in feces which were related to the treatment of malignant ascites by VKR and revealed the biological mechanism of water-expelling effect of VKR. Ultra-fast liquid chromatography-quadrupole-time-of-flight mass spectrometry(UFLC-Q-TOF-MS) was employed to detect the feces of rats in all groups. Principle component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) were conducted to achieve pattern recognition. Combining t-test and variable importance in the projection(VIP) enabled the screening of potential biomarkers for the malignant ascites. Metabolic pathway analysis was accomplished with MetaboAnalyst. Correlation analysis was finally conducted integrating the sequencing data of gut microbiota to elucidate the mechanism underlying the water-expelling effect of VKR. The results showed that both KR and VKR could restore the abnormal metabolism of model rats to some extent, with VKR being inferior to KR in the regulation. Eleven potential biomarkers were identified to be correlated with the malignant ascites and five metabolic pathways were then enriched. Four kinds of gut microbiota were significantly related to the potential biomarkers. The water-expelling effect of VKR may be associated with the regulation of phenylalanine metabolism, biosynthesis of phenylalanine, tyrosine and tryptophan, tryptophan metabolism, glycerophospholipid metabolism, and glycosylphosphatidylinositol(GPI)-anchor biosynthesis. This study can provide a scientific basis for comprehensive understandings of the interaction between gut microbiota and host which has relation to the water-expelling effect of VKR and guide the reasonable clinical application of VKR.


Assuntos
Ácido Acético , Euphorbia , Animais , Ascite/tratamento farmacológico , Ascite/metabolismo , Fezes , Metabolômica , Ratos
3.
Zhongguo Zhong Yao Za Zhi ; 45(6): 1452-1459, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32281360

RESUMO

To reveal the toxic mechanism of Kansui stir-baked with vinegar(VEK), conducta comparative study on the metabolites of fecal samples of rats before and after being treated with chemical constituents group B and C(VEKB/VEKC) extracted from VEK by metabolomics approach. The fecal samples of each group were analyzed using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UFLC-Q-TOF-MS). Then the data was processed by principal component analysis(PCA) and partial least square discriminant analysis(OPLS-DA) to screen and identify biomarkers relating to the toxicity of VEK. Besides, t-test was adopted for univariate statistical analysis, so as to study the changes of these biomarkers in drug groups before and after being treated with VEKB/VEKC and explore the effect of VEKB/VEKC on the metabolism of rat feces. Furthermore, the toxic mechanism of VEKB/VEKC was explored based on the results of the metabolic pathway analysis. The results displayed that compared with control group, the metabolism of fecal samples of VEKB and VEKC treated groups show obvious changes, and the VEKB treated group show more significant changes. A total of 16 potential biomarkers and 5 metabolic pathways relating to the toxicity of VEK were found and identified. And the toxicity of VEK might be associated with the disorder of such metabolic pathways as tryptophan metabolism, primary bile acid biosynthesis, amino sugar and nucleotide sugar metabolism, purine metabolism, and degradation of valine, leucine and isoleucine. This study provides a scientific basis for the clinical safety application of VEK.


Assuntos
Ácido Acético , Euphorbia/química , Fezes/química , Metaboloma , Animais , Biomarcadores , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Ratos
4.
J Ethnopharmacol ; 219: 257-268, 2018 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-29559373

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Kansui, the root of Euphorbia kansui S.L.Liou ex S.B.Ho (E.kansui), is a classical traditional Chinese medicine (TCM) with certain toxicity. According to the theory of TCM, kansui fry-baked wtith vinegar (VEK) possesses low toxicity and mild diuretic and purgative efficacy. In clinical practice, it is commonly used for the treatmtablent of ascites and oliguria. The present study aimed to evaluate the toxicity and efficacy of different fractions of VEK and reveal the underlying material basis by employing an animal model of malignant ascites effusion (MAE) in rats. MATERIALS AND METHODSTA: The MAE rats as the model were constructed in SPF male wistar rats by intraperitoneal injection of Walker-256 tumor cells. The MAE rats were used and randomly divided into the control group (normal rats), control groups with different fractions (VEKA, VEKB, VEKC and VEKD), model group (MAE rats), positive control group (model group with furosemide), model groups with different fractions (VEKA, VEKB, VEKC and VEKD). Histopathological observation was used to confirm Walker-256 tumor-bearing organ injuries in rats. For the efficacy evaluation, the ascites and urine volumes, the urinary electrolyte concentrations (Na+, K+ and Cl-) and pH, the ascites levels of pro-inflammatory cytokines (IL-2, IL-6, TNF-α, IFN-γ and VEGF), PRA, the serum levels of Ang II, ALD and ADH, as well as AQP8 protein expression in the gastrointestinal tract were detected. Furthermore, different levels of indicators were measured in the toxicity evaluation of different fractions both on normal and model rats, including serum liver enzymes (AST and ALT), serum oxidative damage parameters (GSH, MDA, LDH and SOD), expressions of inflammatory parameters (NF-κB, ICAM-1 and E-cadherin) and apoptosis signals (caspase-3, -8, -9, Bcl-2 and Bax) in the liver and gastrointestinal tract. RESULTS: Walker-256 tumor-bearing malignant ascites effusion rats showed obvious hepatic and gastrointestinal injuries by histopathological observation. In the efficacy evaluation, model rats treated with VEKB and VEKC showed significant urine increase (VEKB, P < 0.01; VEKC, P < 0.01) and ascites reduction (VEKB, P < 0.01; VEKC, P < 0.01). These two fractions also balanced the concentrations of Na+, K+ and Cl- in urine (VEKB, all P < 0.05; VEKC, all P < 0.05), remarkably decreased urinary pH (VEKB, P < 0.01; VEKC, P < 0.01), and reduced the ascites levels of IL-2, IL-6, TNF-α, IFN-γ and VEGF (VEKB, all P < 0.01; VEKC, all P < 0.01) in the model rats. Moreover, levels of PRA, the serum Ang II, ALD and ADH of model rats were decreased after treated by VEKB and VEKC (VEKB, all P < 0.05; VEKC, all P < 0.05). Meanwhile, the expression of gastrointestinal AQP8 of the model rats was also enhanced after treated by VEKB and VEKC (VEKB, P < 0.01; VEKC, P < 0.01). In the toxicity evaluation, although VEKB and VEKC caused toxic indexes moved to the worse aspects in normal rats, nearly all of these indicators notably improved in the model rats. Additionally, VEKA showed no effect on the indicators, either in the efficacy evaluation or in the toxicity evaluation. And VEKD could significantly improve some indicators (urine volume, concentration of K+ in urine, serum MDA, AI and caspase-9) in MAE rats. CONCLUSIONS: VEKB and VEKC were demonstrated a significant efficacy in treating malignant ascites effusion, which could reduce hepatic and gastrointestinal damage on the model rats but cause the same damage to the normal. These data embody the traditional Chinese medicine application principle: You Gu Wu Yun. And these results will provide reference for the safer and better clinical utilization of kansui.


Assuntos
Ácido Acético/uso terapêutico , Ascite/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Euphorbia , Raízes de Plantas , Animais , Ascite/metabolismo , Ascite/patologia , Avaliação Pré-Clínica de Medicamentos/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Masculino , Ratos , Ratos Wistar , Resultado do Tratamento
5.
Zhongguo Zhong Yao Za Zhi ; 41(17): 3218-3225, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-28920373

RESUMO

This article summarizes the research progress in recent years on interactions between Chinese medicines and gut microbiota based on the physiological functions of gut microbiota, including imbalance impacts of toxic/irritating Chinese medicines on gut microbiota, prognosis effects of Chinese medicines on gut microbiota imbalance, metabolism effects of gut microbiota on Chinese medicine components, and co-metabolism effects between gut microbiota and host. We would think and prospect the specific biological effects of Chinese medicines, gut microbiota structures and the relations between endogenous metabolites from "gut microbiota and host co-metabolism". All of these aim to investigate biological mechanisms and effective components of Chinese medicines based on gut microbiota and offer a new strategy for promoting safe and effective application of Chinese medicines.


Assuntos
Medicamentos de Ervas Chinesas , Microbioma Gastrointestinal , Humanos , Pesquisa
6.
Zhong Yao Cai ; 37(12): 2189-91, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-26080499

RESUMO

OBJECTIVE: A rapid identification model of the fresh-cut and sulphur fumigation processed Fritillaria thunbergii bulb slices was developed by near-infrared spectroscopy (NIR) with chemometrics method. METHODS: 186 batches of Fritillaria thunbergii bulb slices were collected from the two main producing areas Ningbo and Pan'an in Zhejiang Province, and the near-infrared spectrums were gathered to establish the qualitative identification model by discriminant analysis. RESULTS: The identification model was developed by choosing the spectrum of 9,881.46-4,119.20 cm(-1) and "MSC + spectrum + Ns" to the original spectral preprocessing, and then was verified by prediction set, with 100% identify accuracy. CONCLUSION: The rapid identification model of the fresh-cut and sulphur fumigation processed Fritillaria thunbergii bulb slices by NIR is feasible and efficient.


Assuntos
Fritillaria/classificação , Fumigação , Enxofre , Análise Discriminante , Raízes de Plantas/classificação , Controle de Qualidade , Espectroscopia de Luz Próxima ao Infravermelho
7.
Zhongguo Zhong Yao Za Zhi ; 39(23): 4603-7, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25911809

RESUMO

In order to discriminate the crude and sweated Dipsaci Radix correctly and rapidly, the crude and sweated Dipsaci Radix were scanned by the NIR spectrometer, and an identifying model was developed by near infrared spectroscopy combined with principal component-Mahalanobis distance pattern recognition method. The pretreated spectra data of 129 crude samples and 86 sweated ones were analyzed through principal component analysis (PCA). The identifying model was developed by choosing the spectrum for 9 881.46-4 119.20 cm(-1) and "SNV + spectrum + S-G" to the original spectral preprocessing with 14 principal components, and then was verified by prediction set, identifying with 100% accuracy. The rapid identification model of the crude and sweated Dipsaci Radix by NIR is feasible and efficient, and could be used as an assistant means for identifying the crude and sweated Dipsaci Radix.


Assuntos
Química Farmacêutica/métodos , Dipsacaceae/química , Análise de Componente Principal/métodos , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Raízes de Plantas/química , Controle de Qualidade
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