Removal of nitrate nitrogen by Pseudomonas JI-2 under strong alkaline conditions: Performance and mechanism.

The nitrate nitrogen removal characteristics of Pseudomonas JI-2 under strong alkaline conditions and the composition and functional groups of extracellular polymeric substance were analyzed. Furthermore, nontargeted metabonomics and bioinformatics technology were used to investigate the alkaline tolerance mechanism. JI-2 removed 11.05 mg N/(L·h) of nitrate with the initial pH, carbon to nitrogen ratio and temperature were 11.0, 8 and 25 °C respectively. Even when the pH was maintained at 11.0, JI-2 could still effectively remove nitrate. JI-2 contains a large number of Na+/H+ antiporters, such as Mrp, Mnh (mnhACDEFG) and Pha (phaACDEFG), which can stabilize the intracellular acid-base environment, and SlpA can enable quick adaptation to alkaline conditions. Moreover, JI-2 responds to the strong alkaline environment by secreting more polysaccharides, acidic functional groups and compatible solutes and regulating key metabolic processes such as pantothenate and CoA biosynthesis and carbapenem biosynthesis. Therefore, JI-2 can survive in strong alkaline environments and remove nitrate efficiently.

The coexistence of copper ions and TC affected the binding ability and the reaction order between extracellular polymeric substances of aerobic granular sludge and exogenous substances.

As a barrier against external toxic effects, extracellular polymeric substances (EPSs) directly affect the toxicity and removal efficiency of exogenous substances. The reaction of EPSs with exogenous substances has been taken into consideration. The contents of EPSs in sludge cultivated by different influent water vary greatly, which leads to great differences in the binding ability and reaction sequence between EPSs and exogenous substances. However, the results in this respect are very limited. In this study, the binding characteristics between exogenous tetracycline (TC)/copper ions (Cu2+) and EPSs from aerobic granular sludge cultured under single and coexisting TC/Cu2+ were assessed by three-dimensional fluorescence-parallel factor analysis. The pollutants in the influent water could directionally induce microorganisms to secrete more EPSs, while fluorescence substances in EPSs could combine with the exogenous substances to lessen their effects. In the presence of coexisting TC and Cu2+ in the influent water, the ability of fluorescence substances in EPSs to combine with exogenous TC or Cu2+ weakened, and humic substances in EPSs were more susceptible than protein substances to binding with exogenous substances. However, the reaction order between EPSs components and exogenous TC or Cu2+ was opposite, and the ability of fluorescence substances in EPSs to combine with exogenous TC or Cu2+ was enhanced under individual TC or Cu2+ existing in the influent water. This study provided new insights into the interaction between EPSs and exogenous substances.

Performance and mechanism of nitrate removal by the aerobic denitrifying bacterium JI-2 with a strong autoaggregation capacity.

Here, a new strain JI-2 of the strongly autoaggregating aerobic denitrifying bacteria was screened. The nitrate removal ability and autoaggregation mechanism of JI-2 were analyzed using the nitrogen balance and genomics technology. The nitrate removal rate was 27.05 mg N/(L·h) at pH 9.0 and C/N 8.0. The strain JI-2 removes nitrate via the aerobic denitrification and dissimilation pathways and removes ammonium via the assimilation pathway. 66.81 % nitrate was converted to cellular components under aerobic conditions. Complex nitrogen metabolism genes were detected in strain JI-2. C-di-GMP mediates the motility behavior of JI-2 by binding the FleQ and PilZ proteins, and regulating the expression of PslA. Furthermore, the mechanism of autoaggregation was verified by extracellular polymeric substance analysis. Meanwhile, the nitrate removal rates of strain JI-2 was 11.13-12.50 mg N/(L·h) in wastewater. Thus, strain JI-2 has good prospects for application in the treatment of nitrate wastewater.

Step feed mode synergistic mixed carbon source to improve sequencing batch reactor simultaneous nitrification and denitrification efficiency of domestic wastewater treatment.

The limited efficiency of nitrogen removal has traditionally hindered wide application of simultaneous nitrification and denitrification (SND) technology. Here, the nitrogen removal characteristics of a sequencing batch reactor were studied by adopting a strategy of a step-feeding mode, synergistic regional oxygen limitation, and a mixed carbon source. The changes of the microbial population succession and nitrogen metabolism functional genes were analyzed. This strategy provided a favorable level of dissolved oxygen and continuous carbon sources for driving the denitrification process. The total nitrogen removal efficiency and SND rate reached 92.60% and 96.49%, respectively, by regulating the ratio of sodium acetate to starch in the step feed to 5:1. This procedure increased the relative abundance of denitrifying functional genes and induced the growth of a variety of traditional denitrifying bacteria and aerobic denitrifying bacteria participating in the process of nitrogen removal. Overall, this work offers a new strategy for achieving efficient SND.

Performance and mechanism of tetracycline removal by the aerobic nitrate-reducing strain Pseudomonas sp. XS-18 with auto-aggregation.

The coexistence of multiple pollutants has become a distinctive feature of water pollution. However, there are a few strains that can remove nitrate and tetracycline (TC). Here, the efficiency of strain XS-18 in removing nitrate and TC was analyzed, and the mechanism of tolerance and removal of TC was investigated by infrared spectroscopy, three-dimensional fluorescence spectroscopy, and genome analysis. XS-18 could efficiently remove TC (0.40 mg·L-1·h-1) at pH 7.0-11.0 with auto-aggregation. TC was removed via extracellular polymeric substance (EPS) (55.90%) and cell surface (44.10%) adsorption. TC (10 mg/L) could stimulate XS-18 to secrete more polysaccharides and hydrophobic proteins to improve its auto-aggregation ability. The findings also confirmed that TC resistance genes were present. Furthermore, the bacterial flagellum, signal transduction of the chemotactic system and regulatory genes were shown to be related to the auto-aggregation of the strain. XS-18 has potential applications in the treatment of wastewater containing nitrate and TC.

Nitrate removal by alkali-resistant Pseudomonas sp. XS-18 under aerobic conditions: Performance and mechanism.

To improve poor nitrate removal by microorganisms under strong alkaline conditions, a new type of aerobic nitrification-reducing bacteria was isolated in this study. Using nitrogen balance and genome information, the capacity of Pseudomonas XS-18 to remove nitrate and the mechanism of alkali tolerance were analyzed. At pH 11.0, XS-18 could remove 12.17 mg N/(L·h) nitrate. At C/N ratios of 13.0 and 25 °C, nitrite and ammonia nitrogen were barely enriched. XS-18 could reduce nitrate through dissimilation and assimilation, and 21.74% and 77.39% of nitrate was converted into cellular components and organic nitrogen, respectively. Meanwhile, functional genes (nirBD, nasAB, gdhA, glnA, and gltBD) associated with nitrogen metabolism were determined. In addition, Na+/H+ antiporters (MnhACDEFG, PhaACDEFG, NhaCD and TrkAH) and a cell surface protein (SlpA) from the XS-18 genome, as well as compatible solutes that help stabilize intracellular pH, were also characterized. XS-18 possessed significant potential in alkaline wastewater treatment.

Tetracycline removal in granulation: Influence of extracellular polymers substances, structure, and metabolic function of microbial community.

Tetracycline is a potentially hazardous residual antibiotic detected in various sewages. High concentration (mg/L) of tetracycline is found in pharmaceutical/hospital wastewater and wastewater derived from livestock and poultry. So far, only antibiotics in µg/L level have been reported in granulation of aerobic sludge during wastewater treatment, but its effects in high concentration are rarely reported. In this study, the influence of tetracycline in high concentration (∼2 mg/L) on the formation of granular sludge, structure, and metabolic function of the microbial community during the granulation of aerobic sludge was investigated to improve the understanding of the aerobic granular sludge formation under high-level of tetracycline. The role of extracellular polymers substances (EPSs) derived from granular sludge in the granulation and tetracycline removal process was also investigated, showing that tetracycline improved the relative hydrophobicity, flocculability and protein/polysaccharide ratio of EPSs, accelerating the granulation of sludge. Succession of microbial communities occurred during the domestication of functional bacteria present in the sludge and was accompanied with regulation of metabolic function. The addition of tetracycline lead to an increase of tetracycline-degrading bacteria or antibiotic resistance genus. Those findings provide new perspectives of the influence of tetracycline on aerobic sludge granulation and the removal mechanism of tetracycline.