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1.
Guang Pu Xue Yu Guang Pu Fen Xi ; 37(2): 537-40, 2017 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30291774

RESUMO

With small-scale oil pool experimental and spectral analysis method, the flame spectrum in initial stage of 0(#) diesel oil combustion was conducted a preliminary study for the first time, which was used to develop the technique of intelligent identification and suppression of fire extinguishing at the initial stage of diesel oil combustion. It educed the overall characteristics flame spectrum at the initial stage of 0(#) diesel oil combustion: in 200~380 nm wavelength of near-ultraviolet bands, the spectral intensity is the weakest, the spectral intensity does not change with wavelength, the number of characteristics spectral bands is minimum, the number of obvious characteristics spectral peaks is almost none; in 380~780 nm wavelength of visible bands, the spectral intensity is the strongest, the spectral intensity increases with wavelength, the number of characteristics spectral bands is maximum, the number of obvious characteristics spectral peaks is large; in 780~1 100 nm wavelength of near-infrared bands, the spectral intensity is relatively strong, the inflection point of spectral intensity appears in 780 nm, the appeared intensity decreases with wavelength, the number of characteristics spectral bands is relatively large, the number of obvious characteristics spectral peaks is at a certain number. It is educed through further analysis of the flame spectrum that: the primary intermediate radicals includes OH, CN, CH, C(2), H(2)O, etc; the primary characteristic spectral bands includes the OH racial bands of 3 064  System and Vibration-Rotation bands, the CN racial bands of Violet System and Red System, the CH racial bands of 4 315  System, the C2 racial bands of The Swan system and Phillips Near Infra-red System, the H(2)O molecular Vibration-Rotation bands, etc; the bands and time distribution of primary intermediate radicals and its main generation mechanism; the existence of potassium in this experimental batches of 0(#) diesel oil and the spectral peak of spectral lines in 766 and 769 nm is obvious; the peak of spectral intensity in 431, 512, 516, 547, 589, 766, 769, 891, 927 nm is obvious, and it suitable for the sign of flame identification at the initial stage of 0(#) diesel oil combustion.

2.
Mol Biol Rep ; 39(3): 2677-82, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21691711

RESUMO

In this study, we report the investigation of extracellular fatty acid binding protein gene (Ex-FABP) genetic polymorphism in a sample of 360 chicken individuals. The screening of the coding regions with their intron-exon boundaries and the proximal flanking regions was performed through a PCR-SSCP strategy. Following sequence analysis revealed 35 novel single nucleotide polymorphisms (SNPs) of chicken Ex-FABP gene. Among the 35 SNPs, twenty-five were found in the introns. And the remaining seven and three SNPs were in the coding region and the 5'UTR, respectively. Two SNPs in the coding region caused two missense mutants and the other five did not result in any amino acid changes. The nature and the distribution of Ex-FABP mutations in three chicken breeds were analyzed. Variations detected here might have an impact on Ex-FABP activity and function and underpin the development of gene markers for chicken fatty deposition and metabolism. The polymorphism, generated by C4715T mutation in exon5, was significantly associated with thickness of subcutaneous fat plus skin in cocks. Subcutaneous fat plus skin of cocks was more thick in TT genotype than in CC genotype (P < 0.05). The Ex-FABP gene could be a candidate locus or linked to a QTL that significantly affects fatty deposition and metabolism in chicken.


Assuntos
Proteínas Aviárias/genética , Galinhas/genética , Proteínas de Ligação a Ácido Graxo/genética , Lipocalinas/genética , Polimorfismo de Nucleotídeo Único/genética , Locos de Características Quantitativas/genética , Gordura Subcutânea/metabolismo , Animais , Proteínas Aviárias/metabolismo , Primers do DNA/genética , Proteínas de Ligação a Ácido Graxo/metabolismo , Estudos de Associação Genética/veterinária , Marcadores Genéticos/genética , Lipocalinas/metabolismo , Mutação de Sentido Incorreto/genética , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples
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