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1.
J Oral Microbiol ; 16(1): 2344278, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38686186

RESUMO

Background: Tongue coating microbiota has aroused particular interest in profiling oral and digestive system cancers. However, little is known on the relationship between tongue coating microbiome and colorectal cancer (CRC). Methods: Metagenomic shotgun sequencing was performed on tongue coating samples collected from 30 patients with CRC, 30 patients with colorectal polyps (CP), and 30 healthy controls (HC). We further validated the potential of the tongue coating microbiota to predict the CRC by a random forest model. Results: We found a greater species diversity in CRC samples, and the nucleoside and nucleotide biosynthesis pathway was more apparent in the CRC group. Importantly, various species across participants jointly shaped three distinguishable fur types.The tongue coating microbiome profiling data gave an area under the receiver operating characteristic curve (AUC) of 0.915 in discriminating CRC patients from control participants; species such as Atopobium rimae, Streptococcus sanguinis, and Prevotella oris aided differentiation of CRC patients from healthy participants. Conclusion: These results elucidate the use of tongue coating microbiome in CRC patients firstly, and the fur-types observed contribute to a better understanding of the microbial community in human. Furthermore, the tongue coating microbiota-based biomarkers provide a valuable reference for CRC prediction and diagnosis.

2.
Biopreserv Biobank ; 21(6): 599-609, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36730760

RESUMO

Objective: Recently, researchers have been focusing on characterizing the tongue coating microbiome from patients with digestive tract disease. However, to the best of our knowledge, the tongue coating collection methods have not been standardized until now. This article focuses on bridging this gap by exploring and validating the conditions suitable for the collection of tongue coating samples. Methods: One hundred forty-one healthy subjects were involved in the standardization of the tongue coating collection method. We conducted our standardization experiment by comparing different sampling tools, different preservation solutions, different scraping times, and different storage days with preservation at room temperature. The tongue coating samples from 59 normal individuals were analyzed using 16S ribosomal RNA (rRNA) gene-sequencing technology. The assessment of the quality of extracted DNA was used to verify our established method. We separated the 59 subjects into two groups (aged and younger), and the sequencing results were used to explore the age-related changes in microbiome. Results: Sterile oral swab B is suitable for the collection of tongue coating samples. To obtain a sufficient amount of DNA from a tongue coating sample, we recommend 30 times of tongue coating scraping. Normal saline, phosphate-buffered saline, and commercial preservation solution are all suitable for short-term sample storage (<1 hour). The commercial long-term preservation solution, which stores samples at room temperature (0 hour to 7 days) and can provide for fast commercial transportation, ensures the integrity of the sample DNA as well as the stability of the DNA quality. By using the established method, extracted DNA from all the 59 normal individuals' tongue coating samples passed an appropriate quality bar for microbiome studies. The average value of OD 260/280 is 1.72 ± 0.10; the average total DNA amount is 334.92 ng (±183.81 ng). The bacterial diversity of the tongue coating is increased and the bacterial community composition changes greatly in the NC group (aged normal subjects). Fusobacteriota is found as the dominant bacteria phyla in aged normal subjects with the 16S rRNA gene-sequencing technology. At the genus level, the relative abundance of Fusobacterium, Haemophilus, and Leptotrichia are significantly higher in aged individuals (all p < 0.05), and Neisseria, Streptococcus, and Porphyromonas are significantly higher in younger individuals (all p < 0.05). Conclusion: A participant-friendly tongue coating collection method for microbiome analyses can be established with good reliability and reproducibility. By taking advantage of our established method and 16S rRNA gene sequencing, significant differences were found in diversity and composition of tongue coating microbiota between aged and younger individuals, which contributes to a better understanding of the age-related composition of tongue coating microbiota.


Assuntos
Microbiota , Língua , Humanos , Idoso , Reprodutibilidade dos Testes , RNA Ribossômico 16S/genética , Língua/microbiologia , Microbiota/genética , Bactérias/genética , DNA Bacteriano/genética
3.
Nature ; 533(7602): 200-5, 2016 05 12.
Artigo em Inglês | MEDLINE | ID: mdl-27088604

RESUMO

The whole-genome duplication 80 million years ago of the common ancestor of salmonids (salmonid-specific fourth vertebrate whole-genome duplication, Ss4R) provides unique opportunities to learn about the evolutionary fate of a duplicated vertebrate genome in 70 extant lineages. Here we present a high-quality genome assembly for Atlantic salmon (Salmo salar), and show that large genomic reorganizations, coinciding with bursts of transposon-mediated repeat expansions, were crucial for the post-Ss4R rediploidization process. Comparisons of duplicate gene expression patterns across a wide range of tissues with orthologous genes from a pre-Ss4R outgroup unexpectedly demonstrate far more instances of neofunctionalization than subfunctionalization. Surprisingly, we find that genes that were retained as duplicates after the teleost-specific whole-genome duplication 320 million years ago were not more likely to be retained after the Ss4R, and that the duplicate retention was not influenced to a great extent by the nature of the predicted protein interactions of the gene products. Finally, we demonstrate that the Atlantic salmon assembly can serve as a reference sequence for the study of other salmonids for a range of purposes.


Assuntos
Diploide , Evolução Molecular , Duplicação Gênica/genética , Genes Duplicados/genética , Genoma/genética , Salmo salar/genética , Animais , Elementos de DNA Transponíveis/genética , Feminino , Genômica , Masculino , Modelos Genéticos , Mutagênese/genética , Filogenia , Padrões de Referência , Salmo salar/classificação , Homologia de Sequência
4.
Proc Natl Acad Sci U S A ; 112(44): E5907-15, 2015 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-26483478

RESUMO

The Asian tiger mosquito, Aedes albopictus, is a highly successful invasive species that transmits a number of human viral diseases, including dengue and Chikungunya fevers. This species has a large genome with significant population-based size variation. The complete genome sequence was determined for the Foshan strain, an established laboratory colony derived from wild mosquitoes from southeastern China, a region within the historical range of the origin of the species. The genome comprises 1,967 Mb, the largest mosquito genome sequenced to date, and its size results principally from an abundance of repetitive DNA classes. In addition, expansions of the numbers of members in gene families involved in insecticide-resistance mechanisms, diapause, sex determination, immunity, and olfaction also contribute to the larger size. Portions of integrated flavivirus-like genomes support a shared evolutionary history of association of these viruses with their vector. The large genome repertory may contribute to the adaptability and success of Ae. albopictus as an invasive species.


Assuntos
Aedes/genética , Evolução Molecular , Genoma de Inseto , Aedes/classificação , Aedes/fisiologia , Animais , Filogenia
5.
Nat Commun ; 6: 8212, 2015 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-26354079

RESUMO

Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly heterozygous species that differ in host plant affiliations, and adult and larval colour patterns. Integrating comparative genomics and analyses of gene expression yields multiple insights into butterfly evolution, including potential roles of specific genes in recent diversification. To functionally test gene function, we develop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with three genes, Abdominal-B, ebony and frizzled. Our results provide valuable genomic and technological resources for butterflies and unlock their potential as a genetic model system.


Assuntos
Borboletas/genética , Proteínas de Ligação a DNA/genética , Receptores Frizzled/genética , Genoma de Inseto/genética , Proteínas de Homeodomínio/genética , Proteínas de Insetos/genética , Animais , Sequência de Bases , Sistemas CRISPR-Cas , Perfilação da Expressão Gênica , Técnicas Genéticas , Variação Genética , Dados de Sequência Molecular , Fenótipo
7.
PLoS Genet ; 11(4): e1005118, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25835551

RESUMO

The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral and physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia and air exposure. These traits may render L. crocea a good model for investigating the response mechanisms to environmental stress. To understand the molecular and genetic mechanisms underlying the adaptation and response of L. crocea to environmental stress, we sequenced and assembled the genome of L. crocea using a bacterial artificial chromosome and whole-genome shotgun hierarchical strategy. The final genome assembly was 679 Mb, with a contig N50 of 63.11 kb and a scaffold N50 of 1.03 Mb, containing 25,401 protein-coding genes. Gene families underlying adaptive behaviours, such as vision-related crystallins, olfactory receptors, and auditory sense-related genes, were significantly expanded in the genome of L. crocea relative to those of other vertebrates. Transcriptome analyses of the hypoxia-exposed L. crocea brain revealed new aspects of neuro-endocrine-immune/metabolism regulatory networks that may help the fish to avoid cerebral inflammatory injury and maintain energy balance under hypoxia. Proteomics data demonstrate that skin mucus of the air-exposed L. crocea had a complex composition, with an unexpectedly high number of proteins (3,209), suggesting its multiple protective mechanisms involved in antioxidant functions, oxygen transport, immune defence, and osmotic and ionic regulation. Our results reveal the molecular and genetic basis of fish adaptation and response to hypoxia and air exposure. The data generated by this study will provide valuable resources for the genetic improvement of stress resistance and yield potential in L. crocea.


Assuntos
Adaptação Fisiológica , Proteínas de Peixes/genética , Genoma , Pressão Osmótica , Estresse Oxidativo , Perciformes/genética , Animais , Proteínas de Peixes/metabolismo , Perciformes/metabolismo , Transcriptoma
8.
Nat Commun ; 5: 5594, 2014 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-25463417

RESUMO

Mudskippers are amphibious fishes that have developed morphological and physiological adaptations to match their unique lifestyles. Here we perform whole-genome sequencing of four representative mudskippers to elucidate the molecular mechanisms underlying these adaptations. We discover an expansion of innate immune system genes in the mudskippers that may provide defence against terrestrial pathogens. Several genes of the ammonia excretion pathway in the gills have experienced positive selection, suggesting their important roles in mudskippers' tolerance to environmental ammonia. Some vision-related genes are differentially lost or mutated, illustrating genomic changes associated with aerial vision. Transcriptomic analyses of mudskippers exposed to air highlight regulatory pathways that are up- or down-regulated in response to hypoxia. The present study provides a valuable resource for understanding the molecular mechanisms underlying water-to-land transition of vertebrates.


Assuntos
Anfíbios/genética , Evolução Molecular , Genoma , Perciformes/genética , Amônia/metabolismo , Animais , Perfilação da Expressão Gênica , Hipóxia/genética , Imunidade Inata/genética , Análise de Sequência de DNA , Visão Ocular/genética
9.
Nat Commun ; 5: 3966, 2014 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-24892994

RESUMO

The blind mole rat (BMR), Spalax galili, is an excellent model for studying mammalian adaptation to life underground and medical applications. The BMR spends its entire life underground, protecting itself from predators and climatic fluctuations while challenging it with multiple stressors such as darkness, hypoxia, hypercapnia, energetics and high pathonecity. Here we sequence and analyse the BMR genome and transcriptome, highlighting the possible genomic adaptive responses to the underground stressors. Our results show high rates of RNA/DNA editing, reduced chromosome rearrangements, an over-representation of short interspersed elements (SINEs) probably linked to hypoxia tolerance, degeneration of vision and progression of photoperiodic perception, tolerance to hypercapnia and hypoxia and resistance to cancer. The remarkable traits of the BMR, together with its genomic and transcriptomic information, enhance our understanding of adaptation to extreme environments and will enable the utilization of BMR models for biomedical research in the fight against cancer, stroke and cardiovascular diseases.


Assuntos
Adaptação Fisiológica/genética , Evolução Molecular , Genoma , Hipercapnia , Hipóxia , Spalax/genética , Estresse Fisiológico , Transcriptoma/genética , Animais , Escuridão , Perfilação da Expressão Gênica , Edição de RNA/genética , Elementos Nucleotídeos Curtos e Dispersos
10.
Nat Commun ; 5: 3765, 2014 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-24801114

RESUMO

Spiders are ecologically important predators with complex venom and extraordinarily tough silk that enables capture of large prey. Here we present the assembled genome of the social velvet spider and a draft assembly of the tarantula genome that represent two major taxonomic groups of spiders. The spider genomes are large with short exons and long introns, reminiscent of mammalian genomes. Phylogenetic analyses place spiders and ticks as sister groups supporting polyphyly of the Acari. Complex sets of venom and silk genes/proteins are identified. We find that venom genes evolved by sequential duplication, and that the toxic effect of venom is most likely activated by proteases present in the venom. The set of silk genes reveals a highly dynamic gene evolution, new types of silk genes and proteins, and a novel use of aciniform silk. These insights create new opportunities for pharmacological applications of venom and biomaterial applications of silk.


Assuntos
Genoma/genética , Proteínas de Insetos/genética , Seda/genética , Venenos de Aranha/genética , Aranhas/genética , Animais , Sequência de Bases , Evolução Molecular , Peptídeo Hidrolases/genética , Filogenia , Análise de Sequência de DNA
11.
PLoS One ; 9(4): e95543, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24743270

RESUMO

BACKGROUND: The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced. METHODOLOGY/PRINCIPAL FINDINGS: Genome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana 'Brazil' in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety 'Brazil'. CONCLUSIONS/SIGNIFICANCE: Foc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will thus advance us develop effective methods for managing the banana vascular wilt disease, including improvement of disease resistance in banana.


Assuntos
Proteínas Fúngicas/genética , Fusarium/genética , Fusarium/patogenicidade , Musa/microbiologia , Doenças das Plantas/microbiologia , Transcriptoma/genética , Perfilação da Expressão Gênica , Genoma Fúngico
12.
Nat Commun ; 5: 2957, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24423660

RESUMO

Locusts are one of the world's most destructive agricultural pests and represent a useful model system in entomology. Here we present a draft 6.5 Gb genome sequence of Locusta migratoria, which is the largest animal genome sequenced so far. Our findings indicate that the large genome size of L. migratoria is likely to be because of transposable element proliferation combined with slow rates of loss for these elements. Methylome and transcriptome analyses reveal complex regulatory mechanisms involved in microtubule dynamic-mediated synapse plasticity during phase change. We find significant expansion of gene families associated with energy consumption and detoxification, consistent with long-distance flight capacity and phytophagy. We report hundreds of potential insecticide target genes, including cys-loop ligand-gated ion channels, G-protein-coupled receptors and lethal genes. The L. migratoria genome sequence offers new insights into the biology and sustainable management of this pest species, and will promote its wide use as a model system.


Assuntos
Voo Animal , Regulação da Expressão Gênica , Genoma de Inseto/genética , Locusta migratoria/genética , Animais , Elementos de DNA Transponíveis , Metabolismo Energético , Perfilação da Expressão Gênica
13.
G3 (Bethesda) ; 3(12): 2257-71, 2013 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-24142924

RESUMO

Drosophila suzukii Matsumura (spotted wing drosophila) has recently become a serious pest of a wide variety of fruit crops in the United States as well as in Europe, leading to substantial yearly crop losses. To enable basic and applied research of this important pest, we sequenced the D. suzukii genome to obtain a high-quality reference sequence. Here, we discuss the basic properties of the genome and transcriptome and describe patterns of genome evolution in D. suzukii and its close relatives. Our analyses and genome annotations are presented in a web portal, SpottedWingFlyBase, to facilitate public access.


Assuntos
Proteínas de Drosophila/genética , Drosophila/genética , Genoma de Inseto , Animais , Evolução Biológica , Códon , Elementos de DNA Transponíveis , Feminino , Expressão Gênica , Internet , Masculino , Anotação de Sequência Molecular , Filogenia , Transcriptoma , Navegador
14.
Nat Commun ; 4: 1426, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23385571

RESUMO

Chinese tree shrews (Tupaia belangeri chinensis) possess many features valuable in animals used as experimental models in biomedical research. Currently, there are numerous attempts to employ tree shrews as models for a variety of human disorders: depression, myopia, hepatitis B and C virus infections, and hepatocellular carcinoma, to name a few. Here we present a publicly available annotated genome sequence for the Chinese tree shrew. Phylogenomic analysis of the tree shrew and other mammalians highly support its close affinity to primates. By characterizing key factors and signalling pathways in nervous and immune systems, we demonstrate that tree shrews possess both shared common and unique features, and provide a genetic basis for the use of this animal as a potential model for biomedical research.


Assuntos
Genoma/genética , Tupaia/genética , Animais , China , Variação Genética , Hepacivirus/fisiologia , Hepatite C/genética , Hepatite C/virologia , Humanos , Sistema Imunitário/metabolismo , Inativação Metabólica/genética , Camundongos , Sistema Nervoso/metabolismo , Filogenia , Análise de Sequência de DNA , Tupaia/imunologia
15.
Science ; 339(6118): 456-60, 2013 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-23258410

RESUMO

Bats are the only mammals capable of sustained flight and are notorious reservoir hosts for some of the world's most highly pathogenic viruses, including Nipah, Hendra, Ebola, and severe acute respiratory syndrome (SARS). To identify genetic changes associated with the development of bat-specific traits, we performed whole-genome sequencing and comparative analyses of two distantly related species, fruit bat Pteropus alecto and insectivorous bat Myotis davidii. We discovered an unexpected concentration of positively selected genes in the DNA damage checkpoint and nuclear factor κB pathways that may be related to the origin of flight, as well as expansion and contraction of important gene families. Comparison of bat genomes with other mammalian species has provided new insights into bat biology and evolution.


Assuntos
Evolução Biológica , Quirópteros/genética , Voo Animal , Genoma , Imunidade Inata/genética , Análise de Sequência de DNA , Sequência de Aminoácidos , Animais , Quirópteros/imunologia , Quirópteros/fisiologia , Dano ao DNA/genética , Reparo do DNA/genética , Ecolocação , Evolução Molecular , Especiação Genética , Hibernação/genética , Sequenciamento de Nucleotídeos em Larga Escala , Masculino , Dados de Sequência Molecular , Filogenia , Espécies Reativas de Oxigênio/metabolismo , Seleção Genética , Especificidade da Espécie
16.
Nature ; 490(7418): 49-54, 2012 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-22992520

RESUMO

The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteome of the shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set of genes responding to environmental stress. The expansion of genes coding for heat shock protein 70 and inhibitors of apoptosis is probably central to the oyster's adaptation to sessile life in the highly stressful intertidal zone. Our analyses also show that shell formation in molluscs is more complex than currently understood and involves extensive participation of cells and their exosomes. The oyster genome sequence fills a void in our understanding of the Lophotrochozoa.


Assuntos
Adaptação Fisiológica/genética , Exoesqueleto/crescimento & desenvolvimento , Crassostrea/genética , Genoma/genética , Estresse Fisiológico/fisiologia , Exoesqueleto/química , Animais , Proteínas Reguladoras de Apoptose/genética , Elementos de DNA Transponíveis/genética , Evolução Molecular , Feminino , Regulação da Expressão Gênica no Desenvolvimento/genética , Genes Homeobox/genética , Genômica , Proteínas de Choque Térmico HSP70/genética , Humanos , Larva/genética , Larva/crescimento & desenvolvimento , Espectrometria de Massas , Anotação de Sequência Molecular , Dados de Sequência Molecular , Polimorfismo Genético/genética , Sequências Repetitivas de Ácido Nucleico/genética , Análise de Sequência de DNA , Estresse Fisiológico/genética , Transcriptoma/genética
17.
Mol Biol Evol ; 29(6): 1545-56, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22319135

RESUMO

Analysis of the transcriptome of the kleptoplastic sea slug, Elysia chlorotica, has revealed the presence of at least 101 chloroplast-encoded gene sequences and 111 transcripts matching 52 nuclear-encoded genes from the chloroplast donor, Vaucheria litorea. These data clearly show that the symbiotic chloroplasts are translationally active and, of even more interest, that a variety of functional algal genes have been transferred into the slug genome, as has been suggested by earlier indirect experiments. Both the chloroplast- and nuclear-encoded sequences were rare within the E. chlorotica transcriptome, suggesting that their copy numbers and synthesis rates are low, and required both a large amount of sequence data and native algal sequences to find. These results show that the symbiotic chloroplasts residing inside the host molluscan cell are maintained by an interaction of both organellar and host biochemistry directed by the presence of transferred genes.


Assuntos
Clorófitas/genética , Gastrópodes/genética , Expressão Gênica , Transferência Genética Horizontal , Genes de Cloroplastos , Fotossíntese/genética , Animais , Sequência de Bases , Perfilação da Expressão Gênica , Genoma , Anotação de Sequência Molecular , Dados de Sequência Molecular , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Análise de Sequência de DNA
18.
Gigascience ; 1(1): 16, 2012 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-23587058

RESUMO

BACKGROUND: The pig is an economically important food source, amounting to approximately 40% of all meat consumed worldwide. Pigs also serve as an important model organism because of their similarity to humans at the anatomical, physiological and genetic level, making them very useful for studying a variety of human diseases. A pig strain of particular interest is the miniature pig, specifically the Wuzhishan pig (WZSP), as it has been extensively inbred. Its high level of homozygosity offers increased ease for selective breeding for specific traits and a more straightforward understanding of the genetic changes that underlie its biological characteristics. WZSP also serves as a promising means for applications in surgery, tissue engineering, and xenotransplantation. Here, we report the sequencing and analysis of an inbreeding WZSP genome. RESULTS: Our results reveal some unique genomic features, including a relatively high level of homozygosity in the diploid genome, an unusual distribution of heterozygosity, an over-representation of tRNA-derived transposable elements, a small amount of porcine endogenous retrovirus, and a lack of type C retroviruses. In addition, we carried out systematic research on gene evolution, together with a detailed investigation of the counterparts of human drug target genes. CONCLUSION: Our results provide the opportunity to more clearly define the genomic character of pig, which could enhance our ability to create more useful pig models.

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