RESUMO
In this paper,we have made a preliminary analysis on the necessity,main contents and difficulties in teaching medical undergraduates the principles and applications of optogenetics experimental technology.We focused on theory and practice teaching and emphasized on the most important technological principles,including how to achieve selective intervention of specific neural pathways;how to achieve twoway control of cell excitation or inhibition effect and the main process of the experiment.Finally,we have summarized our recent experience in the teaching process,to provide reference for basic medical experiments teaching in related medical colleges,so as to promote optogenetics experimental teaching for undergraduates in domestic medical colleges.
RESUMO
With the deepening of the reform of management system of higher medical education in China and the comprehensive advancement of quality education , the teaching laboratories in medical colleges have evolved from traditional laboratories serving the needs of a single discipline to medical laboratory centers classified by subject groups and their corresponding functions. Applying the concept of collaborative innovation, practical explorations in such aspects as building a "big experimental teaching center", establishing a new system of experimental teaching courses, exploring a new model of innovative practical education, and building an excellent teacher team engaging in experimental teaching by means of synergic work have been made by the Army Medical University in its construction of an experimental teaching center for basic medical sciences, which also provides practical reference for the construction of experimental teaching centers in the future.
RESUMO
Optogenetics is one of the biggest breakthroughs in neurobiology in recent decades,which is a revolutionary approach to precise intervention of specific cell functions through the combination of optical and genetic engineering techniques.However,this technique involves the interdisciplinary theoretical knowledge and methods,and it requires expensive equipments and long experimental period.Thus,it is not convenient to carry out widely in experiment teaching for undergraduates except the virtual simulation teaching.In present virtual simulation experiment,we aim to learn the basic principle,operation and application of optogenetics and achieve the desired teaching effects by simulating the process of the virus package,simulating the process of the animal selection,surgery and virus stereotactic microinjection,simulating the expression process of photosensitive channel,the extracellular verification process of photosensitive channel function in vivo,and simulating the observation process that optogenetic inhibition of glutamate neurons in the amygdala affect animal fear behavior.In this paper,the content design of the script making of the virtual experiment has been discussed in the above ways.
RESUMO
@#Objective To observe the effect of transcranial low frequency electrical stimulation on the contents of monoamines in ischemic area of rats with middle cerebral artery occlusion(MCAO).MethodsPermanent MCAO model of Wistar rat was established with silk thread enveloped with polyammoniacum.The ischemic areas received various intensity of transcranial low frequency electrical stimulation for 1 hour in rats underwent 1 hour of ischemia.Then the affected tissue was processed with fluorospectrophotometry to determine the contents of dopamine(DA),noradrenalin(NE) and 5-hydroxytryptamine(5-HT).ResultsCompared with the sham-operation group,the contents of DA,NE and 5-HT in ischemic area of MCAO model rats decreased obviously(all P<0.01),while all three monoamines investigated in the sham-operation group with transcranial low frequency electrical stimulation had no significant change.In the MCAO groups stimulating with lower(10 V) and middle(30 V) intensity transcranial low frequency electrical field,the contents of DA,NE and 5-HT in ischemic area had no significant increase.But in the MCAO group stimulating with high(50 V) intensity transcranial low frequency electrical field,the contents of DA,NE and 5-HT in ischemic area increased significantly(P<0.05).ConclusionSome degree of intensity transcranial low frequency electrical field stimulation can increase the contents of DA,NE and 5-HT in ischemic area of rats subjected to MCAO.