RESUMO
Embryonic stem cell is promising for regenerative medicine. However, its application is hampered by the utilization of eggs in most established methods. Recently, a new pluripotent stem cell establishing method was reported that, mouse and human differentiated cells could be induced reprogrammed into a pluripotent state by expressing exogenetic stem factors such as Oct4, Sox2, et al, through retroviral transduction. This approach avoiding egg use is a great breakthrough not only in stem cell technology but also present theory hypothesis of reprogramming. Here these works were reviewed in this article. Both the mechanism of induced reprogramming and the prospects of induced pluripotent stem cells were discussed.
Assuntos
Animais , Humanos , Diferenciação Celular , Genética , Células Cultivadas , Reprogramação Celular , Genética , Fator 3 de Transcrição de Octâmero , Metabolismo , Células-Tronco Pluripotentes , Biologia Celular , Retroviridae , Genética , Fatores de Transcrição SOXB1 , Metabolismo , Transdução GenéticaRESUMO
Human embryonic stem(hES) cells can self-renew and have the ability to differentiate into any type of cells of the body. These characteristics make hES cells a good candidate for cell-based therapies. Current techniques for derivating and culturing embryonic stem cells are very mature. However, concerns arise that pathogen contamination may make these cells unsuitable for therapeutic purposes. An optimal growth environment is greatly needed. Through various in vitro differentiation methods, human embryonic stem cells can be induced into many specialized cell types. However the mechanism of committed differentiation is still unknown.
