RESUMO
In this article, based on the characteristics of tropical area, we analyze the beneficial and the disadvantage of tropical area to human health, points out the existing problems in elderly health management pattern in the tropics area, accordingly we discuss how to establish tropical characterized elderly health management, and put forward constructive suggestions.
RESUMO
BACKGROUND & OBJECTIVE: Endoglin is a marker of tumor angiogenesis. Increasing evidences proved that passive immunotherapy with anti-endoglin monoclonal antibody can effectively inhibit tumor growth, and xenogeneic homologous DNA vaccine can inhibit cross antitumor immunity. This study was to explore the inhibitory effect of a DNA vaccine encoding the extracellular domain of porcine endoglin (ppEDG) on tumor growth in a mouse colon carcinoma model. METHODS: ppEDG was used as a DNA vaccine to actively immunize the colon carcinoma-bearing mice. Tumor volume and survival rate of the mice were observed in 3-day intervals. Microvessel density (MVD) was detected by immunohistochemistry; antibodies against self-endoglin were detected by Western blot and ELISA; the B cells that secrete auto-antibodies against self-endoglin were detected by ELISPOT assay. RESULTS: Eighteen days after tumor cell inoculation, the tumor volume was significantly smaller in ppEDG-immunized group than in empty plasmid (e-p) group and normal saline (NS) group (P<0.05), and the survival time was significantly longer in ppEDG-immunized group than in the control groups (P<0.001). MVD was significantly lower in ppEDG-immunized group than in e-p group and NS group (19.2+/-4.5 vs. 76.9+/-14.4 and 81.4+/-16.9, P<0.001). The antibodies against self-endoglin were identified in ppEDG-immunized group; the major subtypes were IgG(1) and IgG(2b). The auto-antibody-producing B cells were much more in ppEDG-immunized group than in e-p group and NS group (82.5+/-14.1 vs. 3.6+/-1.3 and 4.7+/-2.0, P<0.001). CONCLUSION: ppEDG DNA vaccine could induce the production of auto-antibodies against self-endoglin, which further inhibit angiogenesis and growth of colon carcinoma.
Assuntos
Antígenos CD/genética , Autoanticorpos/sangue , Neoplasias do Colo/patologia , Neovascularização Patológica , Receptores de Superfície Celular/genética , Vacinas de DNA/imunologia , Transferência Adotiva , Animais , Linfócitos B/imunologia , Células COS/imunologia , Vacinas Anticâncer/genética , Vacinas Anticâncer/imunologia , Linhagem Celular Tumoral , Chlorocebus aethiops , Neoplasias do Colo/irrigação sanguínea , Neoplasias do Colo/imunologia , Endoglina , Feminino , Humanos , Imunização , Imunoglobulina G/sangue , Camundongos , Camundongos Endogâmicos BALB C , Transplante de Neoplasias , Plasmídeos , Vacinas de DNA/genéticaAssuntos
Endotélio Vascular/metabolismo , Neoplasias/irrigação sanguínea , Receptores de Superfície Celular/biossíntese , Animais , Células Eucarióticas/metabolismo , Terapia Genética , Neoplasias/terapia , Neovascularização Patológica , Plasmídeos , Receptores de Superfície Celular/genética , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , SuínosRESUMO
OBJECTIVE: To explore a stable technology for inducing ES cell to committed hematopoietic differentiation. METHODS: The effects of various inductive factors on BLast Colony-Forming Cell (BL-CFC) were investigated. In vitro differentiative system for ES cells was employed in this study. RESULTS: A high linear correlation between the number of D3.5 EB-derived cells plated and the number of blast cell colonies was developed, r = 0.9931. With high frequency of blast colonies observed (1.08-1.2 colonies per 100 cells). 20%-30% D4T conditioned medium (D4T CM) showed the most significant growth potentials of blast colonies. D4T CM, EPO or KL alone had no blast colony growth promoting effect (P > 0.05). But VEGF alone had high significant blast colony growth promoting effect (P < 0.001). However, any two factors combination from above four factors exerted better growth promoting effect than VEGF alone (EPO + D4T CM, P < 0.05; KL + D4T CM, P < 0.01; VEGF + D4T CM, P < 0.001). There were no significant difference among VEGF + KL and EPO + D4T CM or KL + D4T CM, and KL + D4T CM (P > 0.05). While the combination of VEGF + D4T CM was better than KL + D4T CM, VEGF + KL or EPO + D4T CM (P < 0.001). Moreover, the combination of VEGF + KL + D4T CM + EPO, had the highest significant blast colony growth promoting effect (P < 0.001). And the highest frequency of blast colonies was observed (1.5-1.2 colonies per 100 cells). CONCLUSION: VEGF may be the main factor which stimulates the growth of significant numbers of blast cell colonies. D4T CM maybe contains strong cofactors. EPO and KL are the main factors for the induction of BL-CFC to committed hematopoietic differentiation. D3.5 EB-derived cells are more sensitive to various stimulators and have strong blast colony growth promoting effect than that of D3.25 EB-derived cells.