RESUMO
Astragalus membranaceus var. mongholicus and Hedysarum polybotrys belong to different genera, but have similar drug efficacy in traditional Chinese medicine theory, and H. polybotrys was used as the legal A. membranaceus var. mongholicus previously. In this study, similarities and differences between them were analyzed via their ITS/ITS2 fragments information. The ITS (internal transcribed spacer) regions were amplified using polymerase chain reaction and then sequenced in two-way. The alignment lengths of ITS regions were 616 bp, in which 508 loci were consistent, and 103 loci were different, accounting for 82.47% and 16.72% of the total ITS nucleotides in length, respectively. As genotype determines phenotype, 1HNMR-based metabolomic approach was further used to reveal the chemical similarities and differences between them. Thirty-four metabolites were identified in the 1H NMR spectra, and twenty-seven metabolites were the common components. Amino acids, carbohydrates and other primary metabolites were similar, while a large difference existed in the flavonoids and astragalosides. This study suggests that A. membranaceus var. mongholicus and H. polybotrys show similarities and differences from molecular and chemical perspectives, which has laid a foundation for elucidating the effective material basis of drug with similar efficacy and resources utilization.
Assuntos
Astragalus propinquus/genética , Fabaceae/genética , Flavonoides/química , Metaboloma , Astragalus propinquus/química , DNA de Plantas/genética , DNA Espaçador Ribossômico/genética , Medicamentos de Ervas Chinesas/química , Fabaceae/química , MetabolômicaRESUMO
As a kind of bioactive natural products with unique space structure, triterpenoid is widely used in diminishing inflammation, antibacterial, and protecting liver and kidney. Because of the strong ability of antitumor, it is likely to be developed as a new generation of anticancer drugs. In this review, the antineoplastic mechanism, structure-activity relationship, and pharmacophore improvement were summarized for further research on the antitumor properties of triterpenoid.
