Isorhamnetin protects endothelial cells model CRL1730 from oxidative injury by hydrogen peroxide.

To explore the protective effect and mechanism of isorhamnetin against oxidative injury caused by H2O2 to endothelial cell strain CRL1730 of human umbilical vein. H2O2 and endothelial cell strain CRL1730 were used, as a model of injured endothelial cell. Three levels of crude drugs areorhamnetin, 22.8µg/ml, 11.4µg/mL and 5.7µg/mL was added to the injured cell strain CRL1730 respectively. The cell injury was measured in terms of necrotic rate, quantities of von Wilebr and factor (vWf) and thrombomodulin (TM), lactate dehydrogenase (LDH) and intracellular free calcium ions through flow cytometry, ELISA, fluorescent spectrometer and laser scanning confocal microscopy respectively. Isorhamnetins @ 11.4µg/mL and 5.7µg/mL has significantly decreased EC necrotic rate, while the increased vWf concentration due to oxidant (200mol/L of H2O2) was significantly decreased by 5.7µg/mL versus 11.4 and 22.8µg/mL isorhamnetin. Also, the increased in TM and LDH in injured cells was reversed to normal level with 5.7 to 11.4µg/mL isorhamnetin. These results suggest that isorhamnetin protect the integrity of cell membranes. Similarly, H2O2 treatment of cells elicited the release of intracellular calcium, however, 5.7µg/mL and 11.4µ g/mL isorhamnetin dramatically inhibited transient release of intracellular calcium. This suggests that isorhamnetin, at lower concentration, could inhibit the IP3-sensitive calcium pool from releasing calcium, protecting VECs from injury by H2O2. Traditional Chinese herbs, hippophaerhamnoides have been recognized as safe and as a source of flavonoids, with strong cardiovascular protection. The results of this study revealed that isorhamnetin produce a strong effect on some targetspresent in ECs and thus, provide a basis for the future work targeted towards endothelial cells protection.

Role of Emotional Stimulation and Endogenous Hydrogen Sulfide in the Development of Atherosclerosis / 中国药师

Objective:To observe the effect of emotional stimulation on the formation process of atherosclerosis ( AS) ,and explain the role of hydrogen sulfide ( H2 S) in atherosclerotic lesions. Methods:Twenty-four Sprague-Dawley rats were randomly divided into AS group, qi-stagnation and blood-stasis AS group and the control group. The AS group was fed with special diets, the qi-stagnation and blood-stasis AS group was fed with special diets and emotional stimulation, and the control group was fed with normal diets. During the experiment, the indicators including the characterization score, H2 S content, four items of the natural bleeding and blood coagula-tion, tissue blood flow and blood lipid were respectively detected in the 4 th, 8 th and 12 th week. Results:Compared with those in the AS group, since the 4 th week, the rats in qi-stagnation and blood-stasis AS group were with significantly decreased activity, slow re-sponse, lackluster fur and dark purple tongue (P<0. 01);the level of plasma lipid increased significantly, and increased further with time extension(P<0. 05 or P<0. 01);since the 8 th week, APTT and FIB changed significantly (P<0. 05),the blood flow to skin, liver and kidney decreased significantly (P<0. 05);the content of H2S was significantly higher in the 12 th week (P<0. 01). Con-clusion:Emotional stimulation promotes the formation of AS model,and the gas molecule system of H2 S plays a regulatory role in the qi-stagnation and blood-stasis AS animal model.

Analysis on establishment and affecting factors of qi stagnation and blood stasis rat model / 中国中药杂志

<p><b>OBJECTIVE</b>To study on the method for establishing the Qi stagnation and blood stasis rat model and analyze the affecting factors.</p><p><b>METHOD</b>The orthogonal design was adopted to study the influences of joint stimulations including noise, light, electricity, ice water bath, tail-clamping on model rats. The 'flying spot' method was used to dynamically simulate blood flow velocity in microcirculation. the pressure sensing technology of MOTO was adopted to detect hemorheology-related indicators. And the coagulation method was used to detect blood coagulation-related indicators.</p><p><b>RESULT</b>Compared with the negative control group, all model groups showed significant reduction in the blood flow velocity in mesenteric microcirculation and increase in the whole blood viscosity at high, medium and low shear rate, the plasma viscosity and the fibrinogen content in four blood coagulation indicators.</p><p><b>CONCLUSION</b>Noise, light, electricity, tail-clamping, bondage and icewater-bath make significant impact on model rats.</p>

Effect of dipfluzine on sodium current in guinea-pig ventricular myocytes / 中国药理学与毒理学杂志

AIM To investigate effect of dipfluzine on sodium current (INa+) in isolated single guinea-pig ventricular myocytes. METHODS INa+ was measured by whole cell patch-clamp technique in single isolated guinea-pig ventricular myocytes which were prepared by enzymatic dissociation method. RESULTSCardiac INa+ was elicited by 50-ms pulses to +50 mV from holding potential at -80 mV with a step of +10 mV, which could be blocked completely by tetrodotoxin 10 μmol·L-1. The peak INa+ occurred at about -20 mV and the reversal potential for INa+ was about +30 mV. Dipfluzine inhibited cardiac INa+ in a concentration-dependent manner. The blocking effect of dipfluzine on INa+ was reversible. Dipfluzine suppressed cardiac INa+, without modifying maximum activation potential and reversal potential. The peak of INa+ was decreased by about 46% at -20 mV and shape of I-V curve was not altered by dipfluzine 40 μmol·L-1. Dipfluzine shifted the steady-state inactivation curve of INa+ towards more negative without changing the slope factor and produced very little change in the steady-state activation curve towards more positive. The mean half activation voltage was (-34.9±5.1) mV and slope factor was (6.0±4.8) mV under control condition and (-33.7±3.6) mV and (5.6±2.4) mV following exposure to dipfluzine 40 μmol·L-1. The half inactivation voltage was (-73.0±4.6)mV and slope factor was (4.8±1.8)mV under control condition and (-82.8±7.2)mV and (4.8±1.8)mV following dipfluzine 40 μmol·L-1 treatment. Dipfluzine delayed recovery of cardiac INa+ from inactivation state. The time course of recovery was (36±11) ms in control group and (79±28) ms in dipfluzine 40 μmol·L-1 group. CONCLUSION Dipfluzine inhi- bits cardiac INa+ in a concentration-dependent manner and has higher affinity for the inactivated state than that for resting state of Na+ channels.

Effect of Zhuang'er Granules on Cellular Immune Function of Mice / 中药新药与临床药理

Objective To study the effect of Zhuang'er Granules(ZG) on cellular immune function of mice.Methods The immunodepression mice models were induced by injection of hydrocortisone.Then the lymphocyte transformation rate was examined by morphological test and phagocyte phagocytosis by in vitro method after 7-day intragastric administration of the drugs.Results Compared with the negative control group,ZG could significantly improve the lymphocyte transformation rate and phagocyte phagocytosis in the normal mice and immunodepression mice(P