Activin A overexpression promotes rat follicular development through SCF-kit-mediated cell signals.

We explored the possible signaling pathway by which activin A induces oocyte maturation. Inhba-overexpressing lentivirus vectors were constructed and transfected into primary granulosa cells in vitro and ovary tissues in vivo in rats. The granulosa cell growth curve was drawn, and antibodies for phospho-Smad2, phospho-Erk5, phospho-Nur77, and stem cell factor (SCF) were prepared for western blot analysis. Protein expression of SCF and C-kit in the rat ovaries was detected by immunohistochemical staining. The rate of granulosa cell proliferation was higher in the Inhba gene overexpression group (INH) than in the control groups (CON group and GFP group) in vitro. Protein expression of SCF and C-kit was higher in the INH group than in the other two groups. phospho-Smad2, phospho-ERK5, P-nur77, and SCF proteins showed positive expression in rat ovarian granulosa cells in each group and were obviously increased in the INH group. Activin A overexpression may promote rat granulosa cell proliferation through Smad2/ERK5/nur77 signaling pathways, and rat granulosa cells overexpressing activin A in vitro showed increased levels of SCF and c-kit proteins.

Application of SOAP in the course of traditional Chinese medicine / 国际中医中药杂志

Summarize the application of SOAP in the medical field and find out its problems in the course of traditional Chinese medicine, to explore the significance and value of SOAP in teaching practice in TCM. Organize relevant literature and discuss the application of SOAP in the course of Chinese medicine. SOAP is widely used in various aspects of medicine, and there are still some problems in the course of TCM. The use of SOAP model for teaching and assessment has important practical significance and value in the course of Chinese medicine internal medicine practice, and SOAP emphasize the practice of the post competence oriented teaching, which is worth promoting.

Chromopeptide A, a highly cytotoxic depsipeptide from the marine sediment-derived bacterium Chromobacterium sp. HS-13-94

A bicyclic depsipeptide, chromopeptide A (1), was isolated from a deep-sea-derived bacterium Chromobacterium sp. HS-13-94. Its structure was determined by extensive spectroscopic analysis and by comparison with a related known compound. The absolute configuration of chromopeptide A was established by X-ray diffraction analysis employing graphite monochromated Mo K α radiation (λ=0.71073 Å) with small Flack parameter 0.03. Chromopeptide A suppressed the proliferation of HL-60, K-562, and Ramos cells with average IC50 values of 7.7, 7.0, and 16.5 nmol/L, respectively.

Changes in the reproductive endocrine function in rat following intraovary microinjection of inhba overexpression lentivirus vectors.

OBJECTIVE: To investigate the reproductive endocrine changes after inhba overexpression into rat ovary. METHOD: Thirty Sprague-Dawley rats were randomly assigned to three groups. Inhba overexpression lentivirus vectors (LV-eGFP-inhba) were microinjected into rat ovary (INH group); Control animals received the same amount lentivirus vector empty (CON group) or LV-eGFP (GFP group). Antral follicle amount and diameter were counted and serum level of activin A, E2, P, FSH, and LH and the expression of ER-α, ER-ß, PR, FSHR, and LHR were measured. RESULTS: There was no significant difference among three groups in antral follicle amount; antral follicle diameter was increased in INH group rats compared with the other group rats. Serum levels of activin A, E2, P, and FSH were increased and LH was decreased in INH group rats compared with the other group rats. The mRNA and protein expression of ER-α, ER-ß, FSHR was higher in INH group rats than that in the other group rats. There was no significant difference in mRNA and protein expression of PR among the three group rats, LHR expression was decreased in INH group rats compared with the other two group rats. CONCLUSION: inhba overexpression in rat ovary in vivo may change reproductive endocrine function.

Relative Analysis of Pseudomonas aeruginosa Strain in Hospital Infection / 中华医院感染学杂志

OBJECTIVE To investigate the relationship of Pseudomonas aeruginosa strain in hospital infection. METHODS ?-Lactamase gene,aminoglycoside modification enzymes(AMEs)gene,and resistant antiseptic(qacE△1-sul1)gene were detected to mutidrug resistant P.aeruginosa with polymerase chain reaction(PCR) and we conducted cluster analysis of the molecule marked drug resistance gene. RESULTS PCR showed the positive ratios of TEM,CARB and VIM genes were 24.32%,43.24%,and 5.4%,respectively,While SHV,CTX-M,OXA-10 group,PER,GES,VEB,DHA,and IMP genes were all negative,the deleted ratio of oprD_2 gene was 86.49%;the positive ratios of aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰgenes were 8.11%,43.24%,18.91%,and 40.54%,respectively and the positive ratio of qacE△1 gene was 51.35%,There were clone transmitted phenomenon. CONCLUSIONS Hospital P.aeruginosa infection can induce clone transmitted hospital infection and it has fulminant prevalence.

Research on Correlation between Selenium Level and Cognitive Function for Rural Elderly Population in China / 医学研究杂志

Objective To evaluate the safeness of this treatment by comparing the serum IGF-Ⅰ and IGFBP3 concentration level before and after receiving rhGH treatment in pediatric patients.Methods 30 pediatric patients in our hospital receiving rhGH treatment were subjected to monitor serum IGF-Ⅰ and IGFBP3 level before and after the treatment and calculate the IGF-Ⅰ and IGFBP3 ratio.At the same time the serum T3,T4 and TSH are also assayed.Results The average increase of booly heights was(8.77?3.01)cm /y(t=7.773,P0.05),but the serum IGFBP3(t=3.759,P0.05).However,there was statistical significance of the serum IGFBP3 level between subgroups(f=22.964 P0.05).Conclusion The serum IGF-Ⅰ and IGFBP3 level are able to be used as one of clinical evaluation benchmarks to detect the risks of inducing tumor during the rhGH treatment in the pediatric patients.

Lentivirus vector-mediated enhanced green fluorescence protein transfection on in vitro corneal epithelial cells of rabbits / 第三军医大学学报

Objective To observe the effect of lentivirus vector-medicated enhanced green fluorescence protein (EGFP) transfection on in vitro corneal epithelial cells.Methods Primary corneal epithelial cells of rabbits were cultured and transfected with lentivirus vector into different multiplicity of infection (MOI) in order to find the best transfection dose.After 24,48,72,and 96 h of transfection,expression of EGFP was observed under microscope and detected by RT-PCR.The transfection rate of corneal epithelial cells was calculated in different MOI.Results EGFP was expressed in corneal epithelial cells 48 h after transfection,which increased with the increasing transfection time.The transfection rate of corneal epithelial cells was 4.5%?0.6%,30.4%?0.9%,51.9%?1.4%,and 75.4%?0.7%,respectively,when MOI was 1,10,50,and 100(P

Detection of Chromosome-encoded ?-Lactamase Gene Families in Klebsiella pneumoniae by Using Nested Polymerase Chain Reaction (nPCR) / 中华医院感染学杂志

OBJECTIVE To survey the chromosome-encoded ?-lactamase gene families: SHV,LEN and OKP in(continuously) isolated Klebsiella pneumoniae in China.METHODS The existence and statistical distribution of all three gene families in antibiotic resistant K.pneumoniae strains were detected by applying nPCR.RESULTS In 44 strains,28 strains were SHV gene positive(63.6%),4 strains were LEN gene positive(9.1%) and none in OKP gene.CONCLUSIONS All the three gene families are chromosome-encoded and have 80-92% of genetic(homogeneity),so they all could identifiy the most important factors assisting the spread of bacterial drug (resistance).nPCR is an economic and convenient measurement to detect the chromosome-encoded ?-lactamase gene.

A CYTOTOXIC METABOLITE OF STREPTOMYCES SP. SC120 / 微生物学通报

During the course of the investigation of the antifungal metabolites against Peronophythora litchii from the fermentation broth of Streptomyces sp SC120, an antibiotics that exhibited the cytotoxicity to CNE 2 was isolated By spectral (UV, IR, HRMS, 1H NMR, 13 C NMR, 1H 1H COSY, HMQC and HMBC) analyses, its structure was identified as pimprinine The cytotoxicity of pimprinine to CNE 2 was reported for the first time