RESUMO
The determination of imidacloprid with DNA via a resonance light scattering (RLS) technique was developed. The RLS of DNA was remarkably quenched after adding imidacloprid in aqueous medium of pH 2.10. An RLS peak at 311 nm was found, and the quenched intensity of RLS at this wavelength was proportional to the concentration of imidacloprid. The linear range of the calibration curve was approximately 0.02-2 microg/mL with the detection limit (S/N = 3) of 0.02 ng/mL. The imidacloprid in river water, cucumbers, and apple samples was determined. The recovery rates were in the range of 91.9% to 95.20%, 97.2% to 111.3%, and 94.5% to 114.8%, respectively. The mechanism of the reaction between imidacloprid and DNA is also discussed.
Assuntos
Técnicas de Química Analítica/métodos , Cucumis sativus/química , DNA/química , Água Doce/química , Imidazóis/química , Malus/química , Nitrocompostos/química , Animais , Bovinos , Monitoramento Ambiental/métodos , Água Doce/análise , Concentração de Íons de Hidrogênio , Inseticidas/química , Limite de Detecção , NeonicotinoidesRESUMO
The interaction between acetamiprid and deoxyribonucleic acid (DNA) was used to determine acetamiprid by resonance light scattering (RLS). The RLS signals of DNA were greatly enhanced by acetamiprid in the spectrum region of 300-600 nm. The spectrum peak is around 316.0 nm. The optimum conditions: pH is 1.73; the concentration of DNA is 2.0 microg x mL(-1)bration curve is 0-2. 25 pg * mLU , with the detection2limit of 0. 2 ig * mL '. The acetamiprid in river water sample was determined. The results were satisfactory, and the recovery rates were in the range of 98%-106%. The interaction mechanism of acetamiprid and DNA was discussed: the interactions between acetamiprid and nucleic acid base include electrostatic effect and Tr-r cumulate effect.
Assuntos
DNA/química , Piridinas/análise , Concentração de Íons de Hidrogênio , Luz , Neonicotinoides , Piridinas/química , Espalhamento de Radiação , Eletricidade EstáticaRESUMO
Analysis of triadimenol was carried out using deoxyribonucleic acids (DNA) via the resonance light scattering (RLS) technique. After adding triadimenol into aqueous medium of pH 1.72, the RLS of DNA was remarkably quenched. A resonance light scattering peak at 310 nm was found, and the quenched intensity of RLS at this wavelength was proportional to the concentration of triadimenol. The linear range of the calibration curve was approximately 0-3 microg mL-1 with a detection limit (S/N=3) of 0.07 microg mL-1. The triadimenol in samples of water, cucumber and human serum was determined. The results were satisfactory, and the recovery rates were in the range of 96.3-106.0%, 94.8-105.9% and 92.3-100.5%, respectively. The interaction mechanism was also studied.
Assuntos
DNA/química , Ácido Clorídrico/química , Luz , Espalhamento de Radiação , Triazóis/análise , Calibragem , Cucumis sativus/química , Água Doce/análise , Fungicidas Industriais/análise , Fungicidas Industriais/sangue , Humanos , Concentração de Íons de Hidrogênio , Concentração Osmolar , Praguicidas/química , Reprodutibilidade dos Testes , Espectrofotometria , Espectrofotometria Ultravioleta , Triazóis/sangue , Triazóis/químicaRESUMO
For the first time, triadimenol was used to determine nucleic acid (DNA) using the resonance light scattering (RLS) technique. The RLS of triadimenol was greatly enhanced by DNA in the range of pH 1.6 to approximately 1.9. A resonance light-scattering peak at 310 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0 to approximately 9 microg/ml with the detection limit of 24 ng ml(-1). The mechanism studies of the system indicated that the enhanced RLS is due to the aggregation of triadimenol on DNA. The nucleic acids in synthetic samples and in rice seedling extraction were analyzed with satisfactory results. Compared with other methods, this method is convenient, rapid, inexpensive and simple.
Assuntos
DNA/química , Espectrometria de Fluorescência , Triazóis , Concentração de Íons de Hidrogênio , Padrões de Referência , Espalhamento de Radiação , Sensibilidade e Especificidade , Espectrometria de Fluorescência/métodosRESUMO
The interaction between deoxyribonucleic acid (DNA) and acetamiprid was studied. It was found that the fluorescence of acetamiprid could be enhanced in the presence of DNA in sulfuric acid solution. The excitation and emission wavelength of acetamiprid was 291 nm and 587 nm, respectively. Under optimal conditions, the calibration graph is over the range of 0.1-10 micromL(-1). The calibration limit is 0.06 microg mL(-1) (S/N = 3). The determination results of DNA in yeast cell and golden staphylococcus samples by this method were satisfactory. The mechanism of the reaction is discussed.
Assuntos
DNA/química , Piridinas/química , Calibragem , DNA/análise , DNA Bacteriano/análise , DNA Bacteriano/química , DNA Fúngico/análise , DNA Fúngico/química , Fluorescência , Estrutura Molecular , Neonicotinoides , Desnaturação de Ácido Nucleico , Espectrometria de Fluorescência , Staphylococcus/genética , Ácidos Sulfúricos , Fatores de Tempo , Leveduras/genéticaRESUMO
For the first time, acetamiprid has been used to determine nucleic acid (DNA) using the resonance light scattering (RLS). The RLS of acetamiprid was greatly enhanced by DNA in the range of pH 1.6-1.8. A RLS peak at 313 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-11.0 microg ml(-1) with the detection limit of 20 ng ml(-1). The nucleic acids in synthetic sample and in rice seedling extraction were determined satisfactorily. The interaction mechanism of acetamiprid and DNA is discussed. Mechanism studies show that the enhanced RLS is due to the aggregation of acetamiprid in the presence of DNA.
