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1.
Nat Biotechnol ; 42(4): 591-596, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37349523

RESUMO

Current N6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.


Assuntos
RNA , Peixe-Zebra , Animais , Camundongos , Peixe-Zebra/genética , Peixe-Zebra/metabolismo , RNA/genética , RNA Mensageiro/genética , Células-Tronco Embrionárias , Células Cultivadas
2.
Proc Natl Acad Sci U S A ; 118(51)2021 12 21.
Artigo em Inglês | MEDLINE | ID: mdl-34921114

RESUMO

N6-methyladenosine (m6A) deposition on messenger RNA (mRNA) controls embryonic stem cell (ESC) fate by regulating the mRNA stabilities of pluripotency and lineage transcription factors (TFs) [P. J. Batista et al., Cell Stem Cell 15, 707-719 (2014); Y. Wang et al., Nat. Cell Biol. 16, 191-198 (2014); and S. Geula et al., Science 347, 1002-1006 (2015)]. If the mRNAs of these two TF groups become stabilized, it remains unclear how the pluripotency or lineage commitment decision is implemented. We performed noninvasive quantification of Nanog and Oct4 TF protein levels in reporter ESCs to define cell-state dynamics at single-cell resolution. Long-term single-cell tracking shows that immediate m6A depletion by Mettl3 knock-down in serum/leukemia inhibitory factor supports both pluripotency maintenance and its departure. This is mediated by differential and opposing signaling pathways. Increased FGF5 mRNA stability activates pErk, leading to Nanog down-regulation. FGF5-mediated coactivation of pAkt reenforces Nanog expression. In formative stem cells poised toward differentiation, m6A depletion activates both pErk and pAkt, increasing the propensity for mesendodermal lineage induction. Stable m6A depletion by Mettl3 knock-out also promotes pErk activation. Higher pErk counteracts the pluripotency exit delay exhibited by stably m6A-depleted cells upon differentiation. At single-cell resolution, we illustrate that decreasing m6A abundances activates pErk and pAkt-signaling, regulating pluripotency departure.


Assuntos
Adenosina/análogos & derivados , Células-Tronco Embrionárias/fisiologia , Sistema de Sinalização das MAP Quinases , Adenosina/metabolismo , Animais , Linhagem Celular , Camadas Germinativas/citologia , Camundongos
3.
Cell Stem Cell ; 16(3): 289-301, 2015 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-25683224

RESUMO

N(6)-methyladenosine (m(6)A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m(6)A modification profiles in the mRNA transcriptomes of four cell types with different degrees of pluripotency. Comparative analysis reveals several features of m(6)A, especially gene- and cell-type-specific m(6)A mRNA modifications. We also show that microRNAs (miRNAs) regulate m(6)A modification via a sequence pairing mechanism. Manipulation of miRNA expression or sequences alters m(6)A modification levels through modulating the binding of METTL3 methyltransferase to mRNAs containing miRNA targeting sites. Increased m(6)A abundance promotes the reprogramming of mouse embryonic fibroblasts (MEFs) to pluripotent stem cells; conversely, reduced m(6)A levels impede reprogramming. Our results therefore uncover a role for miRNAs in regulating m(6)A formation of mRNAs and provide a foundation for future functional studies of m(6)A modification in cell reprogramming.


Assuntos
Adenina/análogos & derivados , Reprogramação Celular/fisiologia , Embrião de Mamíferos/metabolismo , Fibroblastos/metabolismo , Células-Tronco Pluripotentes/metabolismo , Processamento Pós-Transcricional do RNA/fisiologia , Adenina/metabolismo , Animais , Embrião de Mamíferos/citologia , Fibroblastos/citologia , Metilação , Metiltransferases/metabolismo , Camundongos , Camundongos Transgênicos , Células-Tronco Pluripotentes/citologia
4.
Cell Res ; 24(12): 1403-19, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25412662

RESUMO

The role of Fat Mass and Obesity-associated protein (FTO) and its substrate N6-methyladenosine (m6A) in mRNA processing and adipogenesis remains largely unknown. We show that FTO expression and m6A levels are inversely correlated during adipogenesis. FTO depletion blocks differentiation and only catalytically active FTO restores adipogenesis. Transcriptome analyses in combination with m6A-seq revealed that gene expression and mRNA splicing of grouped genes are regulated by FTO. M6A is enriched in exonic regions flanking 5'- and 3'-splice sites, spatially overlapping with mRNA splicing regulatory serine/arginine-rich (SR) protein exonic splicing enhancer binding regions. Enhanced levels of m6A in response to FTO depletion promotes the RNA binding ability of SRSF2 protein, leading to increased inclusion of target exons. FTO controls exonic splicing of adipogenic regulatory factor RUNX1T1 by regulating m6A levels around splice sites and thereby modulates differentiation. These findings provide compelling evidence that FTO-dependent m6A demethylation functions as a novel regulatory mechanism of RNA processing and plays a critical role in the regulation of adipogenesis.


Assuntos
Adenosina/análogos & derivados , Adipócitos/citologia , Adipogenia , Oxigenases de Função Mista/metabolismo , Oxo-Ácido-Liases/metabolismo , Splicing de RNA , RNA Mensageiro/genética , Adenosina/metabolismo , Adipócitos/metabolismo , Dioxigenase FTO Dependente de alfa-Cetoglutarato , Animais , Linhagem Celular , Subunidade alfa 2 de Fator de Ligação ao Core/genética , Subunidade alfa 2 de Fator de Ligação ao Core/metabolismo , Metilação , Camundongos , RNA Mensageiro/metabolismo
5.
Cell Res ; 24(5): 532-41, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24662483

RESUMO

DNA double-strand breaks (DSBs) are highly cytotoxic lesions and pose a major threat to genome stability if not properly repaired. We and others have previously shown that a class of DSB-induced small RNAs (diRNAs) is produced from sequences around DSB sites. DiRNAs are associated with Argonaute (Ago) proteins and play an important role in DSB repair, though the mechanism through which they act remains unclear. Here, we report that the role of diRNAs in DSB repair is restricted to repair by homologous recombination (HR) and that it specifically relies on the effector protein Ago2 in mammalian cells. Interestingly, we show that Ago2 forms a complex with Rad51 and that the interaction is enhanced in cells treated with ionizing radiation. We demonstrate that Rad51 accumulation at DSB sites and HR repair depend on catalytic activity and small RNA-binding capability of Ago2. In contrast, DSB resection as well as RPA and Mre11 loading is unaffected by Ago2 or Dicer depletion, suggesting that Ago2 very likely functions directly in mediating Rad51 accumulation at DSBs. Taken together, our findings suggest that guided by diRNAs, Ago2 can promote Rad51 recruitment and/or retention at DSBs to facilitate repair by HR.


Assuntos
Proteínas Argonautas/metabolismo , Quebras de DNA de Cadeia Dupla , Reparo do DNA , Recombinação Homóloga , Rad51 Recombinase/metabolismo , Linhagem Celular , Humanos , Mapas de Interação de Proteínas , RNA/metabolismo , Ribonuclease III/metabolismo
6.
Nucleic Acids Res ; 42(3): 1593-605, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24214992

RESUMO

DNA methylation has been proven to be a critical epigenetic mark important for various cellular processes. Here, we report that redox-active quinones, a ubiquitous class of chemicals found in natural products, cancer therapeutics and environment, stimulate the conversion of 5 mC to 5 hmC in vivo, and increase 5 hmC in 5751 genes in cells. 5 hmC increase is associated with significantly altered gene expression of 3414 genes. Interestingly, in quinone-treated cells, labile iron-sensitive protein ferritin light chain showed a significant increase at both mRNA and protein levels indicating a role of iron regulation in stimulating Tet-mediated 5 mC oxidation. Consistently, the deprivation of cellular labile iron using specific chelator blocked the 5 hmC increase, and a delivery of labile iron increased the 5 hmC level. Moreover, both Tet1/Tet2 knockout and dimethyloxalylglycine-induced Tet inhibition diminished the 5 hmC increase. These results suggest an iron-regulated Tet-dependent DNA demethylation mechanism mediated by redox-active biomolecules.


Assuntos
Metilação de DNA , Dioxigenases/metabolismo , Ferro/metabolismo , Quinonas/farmacologia , 5-Metilcitosina/metabolismo , Animais , Apoferritinas/biossíntese , Apoferritinas/genética , Linhagem Celular , Linhagem Celular Tumoral , Cloranila/farmacologia , Citosina/análogos & derivados , Citosina/metabolismo , Metilação de DNA/efeitos dos fármacos , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Genoma , Humanos , Camundongos , Oxirredução , Proteínas Proto-Oncogênicas/genética , Quinonas/química
7.
Nat Commun ; 4: 1832, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23673617

RESUMO

Regulation of actomyosin dynamics by post-transcriptional modifications in cytoplasmic actin is still poorly understood. Here we demonstrate that dioxygenase ALKBH4-mediated demethylation of a monomethylated site in actin (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes such as cytokinesis and cell migration. ALKBH4-deficient cells display elevated K84me1 levels. Non-muscle myosin II only interacts with unmethylated actin and its proper recruitment to and interaction with actin depend on ALKBH4. ALKBH4 co-localizes with the actomyosin-based contractile ring and midbody via association with methylated actin. ALKBH4-mediated regulation of actomyosin dynamics is completely dependent on its catalytic activity. Disorganization of cleavage furrow components and multinucleation associated with ALKBH4 deficiency can all be restored by reconstitution with wild-type but not catalytically inactive ALKBH4. Similar to actin and myosin knock-out mice, homozygous Alkbh4 mutant mice display early embryonic lethality. These findings imply that ALKBH4-dependent actin demethylation regulates actomyosin function by promoting actin-non-muscle myosin II interaction.


Assuntos
Actinas/metabolismo , Actomiosina/metabolismo , Carboxiliases/metabolismo , Dioxigenases/metabolismo , Homólogo AlkB 4 da Lisina Desmetilase , Animais , Linhagem Celular , Movimento Celular , Citocinese , Perda do Embrião/metabolismo , Perda do Embrião/patologia , Deleção de Genes , Teste de Complementação Genética , Humanos , Lisina/metabolismo , Metilação , Camundongos , Modelos Biológicos , Ligação Proteica
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