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1.
Zhonghua Gan Zang Bing Za Zhi ; 27(5): 352-357, 2019 May 20.
Artigo em Chinês | MEDLINE | ID: mdl-31177659

RESUMO

Objective: To evaluate the efficacy and safety of sofosbuvir (Nanjing Zhengda Tianqing Pharmaceutical Co., Ltd.) combined with ribavirin in patients with genotype 2 chronic hepatitis C virus infection. Methods: Treatment-naïve or treatment experienced genotype 2 chronic hepatitis C patients from sixteen research centers of China were screened. All subjects received once-daily dose of sofosbuvir (400 mg) combined with ribavirin (body weight < 75 kg, 1 000 mg/day, 400 mg in the morning and 600 mg in the evening; body weight > 75 kg, 1 200 mg/d, 600 mg in the morning and 600 mg in the evening) for 12 weeks. Patients were followed-up for a period of 12 weeks after discontinuation of treatment. Continuous variables were expressed as mean ± standard deviation. The proportion of subjects with virologic response at different follow-up time points and 95% confidence intervals were estimated by maximum likelihood ratio and Clopper-Pearson interval. Results: 132 cases with genotype 2 chronic hepatitis C virus infection from sixteen research centers of China were included, 12 cases of whom were associated with cirrhosis, and the remaining 120 cases were not associated with cirrhosis. One hundred and thirty-one cases completed the study, and one patient lost to follow-up at week 4 after the end of treatment. The sustained virological response rate was 96.2% (95% confidence interval: 92.37% - 99.16%) after 12 weeks of drug withdrawal. Virological relapse occurred in four cases. Of the 132 subjects enrolled in the study, 119 (90.2%) reported 617 adverse events during treatment, of which 359 (76.5%) were TEAE related to sofosbuvir and/or ribavirin. There were nine TEAEs of grade 3 and above, and six cases (4.5%) of them had six severe adverse events. Only one serious adverse event was associated with sofosbuvir and ribavirin (unstable angina pectoris). There were no adverse events leading to drug discontinuation or death. Conclusion: Sofosbuvir combined with ribavirin has a high SVR rate in the treatment of genotype 2 chronic hepatitis C virus infection, and most of the adverse events occurred were mild with acceptable safety profile.


Assuntos
Antivirais/uso terapêutico , Hepacivirus/classificação , Hepatite C Crônica/tratamento farmacológico , Ribavirina/uso terapêutico , Sofosbuvir/uso terapêutico , Antivirais/efeitos adversos , China , Quimioterapia Combinada , Genótipo , Hepacivirus/genética , Hepacivirus/isolamento & purificação , Hepatite C Crônica/complicações , Hepatite C Crônica/virologia , Humanos , Ribavirina/efeitos adversos , Sofosbuvir/efeitos adversos , Resultado do Tratamento
2.
Zhonghua Yi Shi Za Zhi ; 49(1): 55-60, 2019 Jan 28.
Artigo em Chinês | MEDLINE | ID: mdl-30970426

RESUMO

Li Shicai, a famous physician of Ming Dynasty had a large number of students. Shen Langzhong, who was a student of Li Shicai, was the teacher of Ma Yuanyi, and one of Ma's student was You Zaijing. This inheritance pedigree was called "Li Shicai School" in academic communities. There were little of study on its later physicians after You Zaijing. This paper collated the medical works, genealogy, local chronicles and medical records of Li Shicai and doctors of different generations. We clarified the academic inheritance genealogy of the past four hundred years. Up to now, there have been twelve generations totally.

3.
Clin Exp Immunol ; 176(1): 102-11, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24372395

RESUMO

Transforming growth factor (TGF)-ß, type I receptor (TßRI) and c-Jun N-terminal kinases (JNK) phosphorylate Smad3 differentially to create 2 isoforms phosphorylated (p) at the COOH-terminus (C) or at the linker region (L) and regulate hepatocytic fibrocarcinogenesis. This study aimed to compare the differences between how hepatitis B virus (HBV) infection affected hepatocytic Smad3 phosphorylated isoforms before and after anti-viral therapy. To clarify the relationship between Smad3 phosphorylation and liver disease progression, we studied 10 random patients in each stage of HBV-related fibrotic liver disease (F1-4) and also 10 patients with HBV-associated HCC. To examine changes in phosphorylated Smad3 signalling before and after anti-HBV therapies, we chose 27 patients with chronic hepatitis B who underwent baseline and follow-up biopsies at 52 weeks from the start of nucleoside analogue treatments (Lamivudine 100 mg daily or Telbivudine 600 mg daily). Fibrosis stage, inflammatory activity and phosphorylated Smad3 positivity in the paired biopsy samples were compared. Hepatocytic pSmad3C signalling shifted to fibrocarcinogenic pSmad3L signalling as the livers progressed from chronic hepatitis B infection to HCC. After nucleoside analogue treatment, serum alanine aminotransferase (ALT) and HBV-DNA levels in 27 patients with HBV-related chronic liver diseases were decreased dramatically. Decrease in HBV-DNA restored pSmad3C signalling in hepatocytes, while eliminating prior fibrocarcinogenic pSmad3L signalling. Oral nucleoside analogue therapies can suppress fibrosis and reduce HCC incidence by successfully reversing phosphorylated Smad3 signalling; even liver disease progressed to cirrhosis in chronic hepatitis B patients.


Assuntos
Carcinoma Hepatocelular/metabolismo , Hepatite B Crônica/complicações , Cirrose Hepática/metabolismo , Neoplasias Hepáticas/metabolismo , Proteína Smad3/metabolismo , Adulto , Antivirais/uso terapêutico , Carcinoma Hepatocelular/complicações , Carcinoma Hepatocelular/tratamento farmacológico , DNA Viral/sangue , Progressão da Doença , Feminino , Vírus da Hepatite B/efeitos dos fármacos , Vírus da Hepatite B/genética , Vírus da Hepatite B/fisiologia , Hepatite B Crônica/tratamento farmacológico , Hepatite B Crônica/virologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Hepatócitos/patologia , Humanos , Imuno-Histoquímica , Lamivudina/uso terapêutico , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/virologia , Cirrose Hepática/complicações , Cirrose Hepática/tratamento farmacológico , Neoplasias Hepáticas/complicações , Neoplasias Hepáticas/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Fosforilação/efeitos dos fármacos , Isoformas de Proteínas/metabolismo , Transdução de Sinais/efeitos dos fármacos , Telbivudina , Timidina/análogos & derivados , Timidina/uso terapêutico , Resultado do Tratamento , Adulto Jovem
4.
Mol Plant Microbe Interact ; 14(2): 234-41, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11204787

RESUMO

Hypersensitive reaction and pathogenicity (hrp) genes are required for Pseudomonas syringae pv. tomato (Pst) DC3000 to cause disease in susceptible tomato and Arabidopsis thaliana plants and to elicit the hypersensitive response in resistant plants. The hrp genes encode a type III protein secretion system known as the Hrp system, which in Pst DC3000 secretes HrpA, HrpZ, HrpW, and AvrPto and assembles a surface appendage, named the Hrp pilus, in hrp-gene-inducing minimal medium. HrpA has been suggested to be the Hrp pilus structural protein on the basis of copurification and mutational analyses. In this study, we show that an antibody against HrpA efficiently labeled Hrp pili, whereas antibodies against HrpW and HrpZ did not. Immunogold labeling of bacteria-infected Arabidopsis thaliana leaf tissue with an Hrp pilus antibody revealed a characteristic lineup of gold particles around bacteria and/or at the bacterium-plant contact site. These results confirm that HrpA is the major structural protein of the Hrp pilus and provide evidence that Hrp pili are assembled in vitro and in planta.


Assuntos
Arabidopsis/microbiologia , Proteínas da Membrana Bacteriana Externa/metabolismo , Fímbrias Bacterianas , Pseudomonas/metabolismo , Ágar , Proteínas da Membrana Bacteriana Externa/genética , Meios de Cultura , Imuno-Histoquímica , Mutação , Folhas de Planta/microbiologia , Folhas de Planta/ultraestrutura
5.
Proc Natl Acad Sci U S A ; 97(5): 2247-52, 2000 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-10681465

RESUMO

Bacterial surface appendages called pili often are associated with DNA and/or protein transfer between cells. The exact function of pili in the transfer process is not understood and is a matter of considerable debate. The Hrp pilus is assembled by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato (Pst) strain DC3000. In this study, we show that the hrpA gene, which encodes the major subunit of the Hrp pilus, is required for secretion of putative virulence proteins, such as HrpW and AvrPto. In addition, the hrpA gene is required for full expression of genes that encode regulatory, secretion, and effector proteins of the type III secretion system. hrpA-mediated gene regulation apparently is through effect on the mRNA level of two previously characterized regulatory genes, hrpR and hrpS. Ectopic expression of the hrpRS gene operon restored gene expression, but not protein secretion, in the hrpA mutant. Three single amino acid mutations at the HrpA carboxyl terminus were identified that affect the secretion or regulatory function of the HrpA protein. These results define an essential role of the Hrp pilus structural gene in protein secretion and coordinate regulation of the type III secretion system in Pst DC3000.


Assuntos
Proteínas de Bactérias/genética , Proteínas de Ligação a DNA/genética , Proteínas de Escherichia coli , Regulação Bacteriana da Expressão Gênica , Pili Sexual , Polissacarídeo-Liases/genética , Pseudomonas/genética , RNA Helicases , Fatores de Transcrição , Aminoácidos , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/fisiologia , RNA Helicases DEAD-box , Genes Bacterianos , Mutagênese , Óperon , Polissacarídeo-Liases/metabolismo , RNA Bacteriano , Transcrição Gênica
6.
Shi Yan Sheng Wu Xue Bao ; 32(1): 77-85, 1999 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-12548798

RESUMO

HarpinPss can induce hypersensitive reaction (HR) in tobacco leaves. As superoxide dismutase can inhibit but catalase can not inhibit the development of HR induced by harpinPss, superoxide anion is required for this response. HarpinPss can also induce the release of active oxygen and extracellular alkalinization, two early defence responses in tobacco suspension culture. Diphenylene iodoium, can completely inhibit the induction of HR in tobacco leaves, and the release of active oxygen in the suspension culture system, superoxide anion in these system may be produced by the activation of NADPH oxidase. Ethyleneglycol-bis (beta-aminoethyl) N, N, N'N'-tetraacetic acid (EGTA) can inhibit the development of harpinPss-induced HR and these two early defence responses in suspension culture system. Adding Ca2+ into the medium again, these responses can return to normal level in a short time. Lanthanum chloride, verapamil, neomycin, U-73122, and LiCl can also inhibit these harpinPss-induced responses. Therefore, the influx of Ca2+ mediated by calcium channel and the release of Ca2+ from internal Ca2+ pool may be involved in the two early defense responses induced by harpinPss. Cycloheximide and actinomycin D have no effect on the release of active oxygen but can inhibit harpinPss-induced HR even added them in the intermediate process for inducing HR. It indicates superoxide is just a trigger for HR, and HR is a more complex process that needs the sustained expression of some genes.


Assuntos
Proteínas da Membrana Bacteriana Externa/farmacologia , Cálcio/metabolismo , Nicotiana/fisiologia , Plantas Tóxicas , Canais de Cálcio/fisiologia , Células Cultivadas , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Nicotiana/efeitos dos fármacos
7.
Shi Yan Sheng Wu Xue Bao ; 32(1): 88-92, 1999 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-12548799

RESUMO

No matter when anion channel inhibitors, DIDS (4, 4'-diisothiocyanatostilbene-2, 2'-disulfonic acid) and A9C (anthracene-9-carboxylic acid) added (before, at the same time of or after harpinPss treatment), they can inhibit harpinPss-induced hypersensitive response in tobacco seedlings and release of active oxygen and extracellular alkalinization in tobacco suspension cells. DIDS and A9C also inhibit harpinPss-induced Ca2+ influx. In all these cases, DIDS is more efficient than A9C. It is postulated that anion channel positively regulates calcium channel in plasma membrane, and harpinPss may function through signal transduction mediated by anion channel and calcium channel to regulate cellular Ca2+ concentration and defense responses.


Assuntos
Proteínas da Membrana Bacteriana Externa/farmacologia , Cálcio/metabolismo , Canais Iônicos/fisiologia , Nicotiana/fisiologia , Plantas Tóxicas , Ânions , Canais de Cálcio/fisiologia , Células Cultivadas , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/fisiologia , Nicotiana/efeitos dos fármacos
8.
Chin Med J (Engl) ; 102(12): 944-6, 1989 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-2517741

RESUMO

A case of hyaline membrane disease was treated successfully with pulmonary surfactant (PS) isolated from human amniotic fluid. Dosage was 150 mg phospholipid/kg. The exogenous surfactant was instilled into the airway via a tracheal cannula. Clinical symptoms, PO2 and FiO2 improved evidently 24 hours after administration. L/S ratio and phosphatidylglycerol recovered gradually in aspirates. Lung X-ray film manifested "white lung" before instillation of surfactant and showed a striking improvement 3 days after treatment. The duration of mechanical ventilation was 6 days. During the period of recovery complications of patent ductus arteriosus and bacterial pneumonia developed. However, the patient recovered completely and was discharged 32 days after admission.


Assuntos
Doença da Membrana Hialina/terapia , Surfactantes Pulmonares/uso terapêutico , Líquido Amniótico/análise , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Surfactantes Pulmonares/isolamento & purificação
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