Target-driven cascade amplified assembly of covalent organic frameworks on tetrahedral DNA nanostructure with multiplex recognition domains for ultrasensitive detection of microRNA.

BACKGROUND: MicroRNA (miRNA) emerges as important cancer biomarker, accurate detection of miRNA plays an essential role in clinical sample analysis and disease diagnosis. However, it remains challenging to realize highly sensitive detection of low-abundance miRNA. Traditional detection methods including northern blot and real-time PCR have realized quantitative miRNA detection. However, these detection methods are involved in sophisticated operation and expensive instruments. Therefore, the development of novel sensing platform with high sensitivity and specificity for miRNA detection is urgently demanded for disease diagnosis. RESULTS: In this work, a novel electrochemical biosensor was constructed for miRNA detection based on target-driven cascade amplified assembly of electroactive covalent organic frameworks (COFs) on tetrahedral DNA nanostructure with multiplex recognition domains (m-TDN). COFs were employed as nanocarriers of electroactive prussian blue (PB) molecules by the "freeze-drying-reduction" method without the use of DNA as gatekeeper, which was simple, mild and efficient. The target-triggered catalytic hairpin assembly (CHA) and glutathione reduction could convert low-abundance miRNA into a large amount of Mn2+. Without the addition of exogenous Mn2+, the dynamically-generated Mn2+-powered DNAzyme cleavage process induced abundant PB-COFs probe assembled on the four recognition domains of m-TDN, resulting in significantly signal output. Using miRNA-182-5p as the model target, the proposed electrochemical biosensor achieved ultrasensitive detection of miRNA-182-5p in the range of 10 fM-100 nM with a detection limit of 2.5 fM. SIGNIFICANCE AND NOVELTY: Taking advantages of PB-COFs probe as the enhanced signal labels, the integration of CHA, Mn2+-powered DNAzyme and m-TDN amplification strategy significantly improved the sensitivity and specificity of the biosensor. The designed sensing platform was capable of miRNA detection in complex samples, which provided a new idea for biomarker detection, holding promising potential in clinical diagnosis and disease screening.

Frictionless multiphasic interface for near-ideal aero-elastic pressure sensing.

Conventional pressure sensors rely on solid sensing elements. Instead, inspired by the air entrapment phenomenon on the surfaces of submerged lotus leaves, we designed a pressure sensor that uses the solid-liquid-liquid-gas multiphasic interfaces and the trapped elastic air layer to modulate capacitance changes with pressure at the interfaces. By creating an ultraslippery interface and structuring the electrodes at the nanoscale and microscale, we achieve near-friction-free contact line motion and thus near-ideal pressure-sensing performance. Using a closed-cell pillar array structure in synergy with the ultraslippery electrode surface, our sensor achieved outstanding linearity (R2 = 0.99944 ± 0.00015; nonlinearity, 1.49 ± 0.17%) while simultaneously possessing ultralow hysteresis (1.34 ± 0.20%) and very high sensitivity (79.1 ± 4.3 pF kPa-1). The sensor can operate under turbulent flow, in in vivo biological environments and during laparoscopic procedures. We anticipate that such a strategy will enable ultrasensitive and ultraprecise pressure monitoring in complex fluid environments with performance beyond the reach of the current state-of-the-art.

400nm ultra-broadband gratings for near-single-cycle 100 Petawatt lasers.

Compressing high-energy laser pulses to a single-cycle and realizing the "λ3 laser concept", where λ is the wavelength of the laser, will break the current limitation of super-scale projects and contribute to the future 100-petawatt and even Exawatt lasers. Here, we have realized ultra-broadband gold gratings, core optics in the chirped pulse amplification, in the 750-1150 nm spectral range with a > 90% -1 order diffraction efficiency for near single-cycle pulse stretching and compression. The grating is also compatible with azimuthal angles from -15° to 15°, making it possible to design a three-dimensional compressor. In developing and manufacturing processes, a crucial grating profile with large base width and sharp ridge is carefully optimized and controlled to dramatically broaden the high diffraction efficiency bandwidth from the current 100-200 nm to over 400 nm. This work has removed a key obstacle to achieving the near single-cycle 100-PW lasers in the future.

Facile Transfer of a Transparent Silver Nanowire Pattern to a Soft Substrate Using Graphene Oxide as a Double-Sided Adhesion-Tuning Layer.

Silver nanowires (AgNWs) have been employed in various optoelectronic devices as transparent electrodes. However, it remains a great challenge to facilely pattern silver nanowires to realize desirable soft skin devices. Here, we develop an intact transfer method via a double-layered adhesion regulator of graphene oxide (GO) enabling complete transfer of a silver nanowire pattern from a tough substrate onto soft polydimethylsiloxane (PDMS) and flexible polyethylene (PE). We achieve positive and negative patterns simultaneously when selectively transferring silver nanowire patterns. The resulting patterned AgNW electrodes have uniform conductivity and long-term stability. The underlying mechanism of the clean transfer is thoroughly investigated via transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). GO plays a role in reducing the adhesion of AgNW to the donor tough substrate and enhancing adhesion of AgNW to the target soft substrate simultaneously. Finally, we demonstrate the utility of the patterned electrodes as transparent sensors detecting body motion. This work offers an effective solution to the challenging patterning problem of silver nanowires on a hydrophobic soft substrate, which is compatible with the soft component in emerging smart skin or wearable electronics.

Zinc-Air Battery-Based Self-Powered Sensor with High Output Power for Ultrasensitive MicroRNA let-7a Detection in Cancer Cells.

Self-powered sensors do not require a power supply and are easy to miniaturize, which have potential for constructing wearable, portable, and real-time detection devices. However, it is challenging for the detection of low abundant targets due to the low output power density of fuel cells and much interference of complex biological environment. Herein, a new kind of photocatalytic zinc-air battery-based self-powered electrochemical sensor (ZAB-SPES) was constructed for the detection of microRNA let-7a (miRNA let-7a) by combining magnetic nanobeads (MBs) with a metal-organic framework loaded with glucose oxidase (MOFs@GOX). Poly(1,4-di(2-thienyl))benzene (PDTB) was used as the photocathode material, and the proposed ZAB-SPES had a high power density of 22.8 µW/cm2, which was 2-3-fold of commonly used photofuel cells. MBs can capture and separate miRNA from complex samples quickly with a high separation efficiency of 99% within 60 s. The competitive reaction of oxygen reduction reaction between PDTB and MOFs@GOX would change the output power density of the ZAB-SPES. Based on the relationship between output power density and target concentration, the ZAB-SPES realized ultrasensitive detection of miRNA let-7a with a detection limit down to 1.38 fM. Furthermore, the successful detection of miRNA let-7a in A549 cancer cells indicated the great prospects of ZAB-SPES in clinical analysis and early diagnosis of cancers.

Needle-integrated ultrathin bioimpedance microsensor array for early detection of extravasation.

Extravasation is a common complication during intravenous therapy in which infused fluids leak into the surrounding tissues. Timely intervention can prevent severe adverse consequences, but early detection remains an unmet clinical need because existing sensors are not sensitive to leakage occurring in small volumes (< 200 µL) or at deep venipuncture sites. Here, an ultrathin bioimpedance microsensor array that can be integrated on intravenous needles for early and sensitive detection of extravasation is reported. The array comprises eight microelectrodes fabricated on an ultrathin and flexible polyimide substrate as well as functionalized using poly(3,4-ethylenedioxythiophene) and multi-walled carbon nanotubes. Needle integration places the array proximity to venipuncture site, and functional coating significantly reduces interface impedance, both enable the microsensors with high sensitivity to detect early extravasation. In vitro and in vivo experiments demonstrate the capability of the microsensors to differentiate various intravenous solutions from different tissue layers as well as identify saline extravasation with detection limit as low as 20 µL.

A wireless optoelectronic skin patch for light delivery and thermal monitoring.

Wearable optoelectronic devices can interface with the skin for applications in continuous health monitoring and light-based therapy. Measurement of the thermal effect of light on skin is often critical to track physiological parameters and control light delivery. However, accurate measurement of light-induced thermal effects is challenging because conventional sensors cannot be placed on the skin without obstructing light delivery. Here, we report a wearable optoelectronic patch integrated with a transparent nanowire sensor that provides light delivery and thermal monitoring at the same location. We achieve fabrication of a transparent silver nanowire network with >92% optical transmission that provides thermoresistive sensing of skin temperature. By integrating the sensor in a wireless optoelectronic patch, we demonstrate closed-loop regulation of light delivery as well as thermal characterization of blood flow. This light delivery and thermal monitoring approach may open opportunities for wearable devices in light-based diagnostics and therapies.

Low-dispersion mirror with a broad bandwidth and high laser damage resistance.

A low-dispersion mirror (LDM), an important component in ultrafast laser systems, requires both a broad low-dispersion laser-induced damage threshold (LIDT). It is difficult for a traditional quarter-wavelength-based dielectric LDM to achieve these characteristics at the same time. We propose a novel, to the best of our knowledge, low-dispersion mirror (NLDM) that combines periodic chirped layers at the top and alternating quarter-wavelength layers at the bottom. Low dispersion is achieved by introducing a large same group delay (GD) for different wavelengths, so the bandwidth is broadened greatly. In addition, owing to the staggered electric field intensity peak effect in the structure, the NLDM shows the potential for high laser damage resistance. The experiments demonstrated that the NLDM doubles the low-dispersion bandwidth, while the LIDT is also increased compared with the LDM. This novel concept results in improved performance and paves the way toward a new generation of the LDM for ultrafast bandwidth and a high laser applications.

Optical vortex switch based on multiplexed volume gratings with high diffraction efficiency.

Systems of controllable orbital angular momentum (OAM) require more compact, higher conversion efficiency and more tolerable wavelength or polarization. We introduce an optical vortex switch based on a multiplexed volume grating (MVG). The MVG recorded in a piece of photo-thermo-refractive (PTR) glass exhibits high diffraction efficiency (DE, also known as conversion efficiency in transporting), sensitive angular selectivity, and polarization-insensitivity. The effects of the incident divergence angle and polarization on the DE and the far-field diffraction profiles are demonstrated and investigated. It turns out that the divergence angle of the probe beam can greatly affect the DE. The fluctuation of the DE caused by polarization variation is less than 1.59%. This switch can be potentially applied in vortex tweezers, optical communication, and high power systems.

Photocatalytic Fuel Cell-Assisted Molecularly Imprinted Self-Powered Sensor: A Flexible and Sensitive Tool for Detecting Aflatoxin B1.

The self-powered electrochemical sensor has gained big achievements in energy and devices, but it is challenging in analytical application owing to its low energy conversion efficiency and limited selectivity caused by the plentiful interference in actual samples. Herein, a new self-powered biosensor was constructed by the integration of a photocatalytic fuel cell (PFC) with a molecular imprinting polymer (MIP) to achieve sensitive and specific detection of aflatoxin B1 (AFB1). Compared with other fuel cells, the PFC owns the advantages of low cost, high energy, good stability, and friendly environment by using light as the excitation source. MoS2-Ti3C2Tx MXene (MoS2-MX) served as the photoanode material for the first time by forming a heterojunction structure, which can enhance the photocurrent by about 3-fold and greatly improve the photoelectric conversion efficiency. Aiming at the poor selectivity of the self-powered sensor, the MIP was introduced to achieve the specific capture and separation of targets without sample pretreatment. Using the MIP and PFC as recognition and signal conversion elements, respectively, the proposed self-powered biosensor showed a wide dynamic range of 0.01-1000 ng/mL with a detection limit of 0.73 pg/mL, which opened opportunities to design more novel self-powered biosensors and promoted its application in food safety and environmental monitoring.

Effect of the interface on femtosecond laser damage of a metal-dielectric low dispersion mirror.

Metal-dielectric low dispersion mirrors (MLDM) have a promising application prospect in petawatt (PW) laser systems. We studied the damage characteristics of MLDM and found that the damage source of MLDM (Ag + Al2O3+SiO2) is located at the metal-dielectric interface. We present the effect of the interface on the femtosecond laser damage of MLDM. Finite element analysis shows that thermal stress is distributed at the interface, causing stress damage which is consistent with the damage morphology. After enhancing the interface adhesion and reducing the residual stress, the damage source transfers from the interface to a surface SiO2 layer, and the damage threshold can be increased from 0.60 J/cm2 to 0.73 J/cm2. This work contributes to the search for new techniques to improve the damage threshold of MLDM used in PW laser systems.

Direct measurement of radial fluence distribution inside a femtosecond laser filament core.

Modulation and direct measurement of the radial fluence distribution inside a single filament core (especially less than 100 µm in diameter) is crucial to filament-based applications. We report direct measurements of the radial fluence distribution inside a femtosecond laser filament core and its evolution via the filament-induced ablation method. The radial fluence distributions were modulated by manipulating the input pulse diffraction through an iris. Compared with using a traditionally circular iris, a stellate iris substantially suppressed the diffraction effect, and laser fluence, intensity and plasma density inside the filament core were considerably increased. The radial fluence inside filament cores was also quantitatively measured via the filament drilling diaphragms approach. Furthermore, numerical simulations were performed to support the experimental results by solving nonlinear Schrödinger equations. The effects of the tooth size of the stellate iris were numerically investigated, which indicated that bigger tooth favors higher fluence and longer filament. In addition to being beneficial in understanding the filamentation process and its control, the results of this study can also be valuable for filament-based applications.

Accurate temperature measurement of a spectral beam combination grating based on VO2 film.

In a spectral beam combination system, temperature increase of the multilayer dielectric grating (MDG) worsens the far-field beam quality of the output laser. To accurately monitor the surface temperature of the MDG, this study deposits VO2 phase-change film on the lowest layer of multilayer dielectric films in the MDG and tests the transmittance with a probe laser. Based on this measurement, the surface temperature of the MDG can be calculated. Additionally, the study analyzes the influence of VO2 film on the surface electric field and the -1 diffraction efficiency of the MDG and presents a specific example of using VO2 film to test high reflector temperature. The study concludes that VO2 film is a feasible method of measuring temperature and better than an infrared thermal imager.

Template-free synthesis and lithium-ion storage performance of multiple ZnO nanoparticles encapsulated in hollow amorphous carbon shells.

Due to the limited utilization of electrode materials, the rational design and facile synthesis of composite structures are still challenging issues for lithium-ion batteries (LIBs). Herein, a simple approach has been developed to prepare multiple core-shell structures of ZnO nanoparticles (NPs) encapsulated in hollow amorphous carbon (AC) shells. The as-synthesized ZnO@AC composites showed a uniform dispersion of ZnO NPs, compliant buffer AC shells, and nanoscale void spaces between the ZnO NP cores and AC shells. As a result of their structural merits, the ZnO@AC composites were evaluated as anode materials for LIBs and delivered enhanced coulombic efficiency, high reversible capacity, high rate capability, and improved cycling stability.

Fabrication of high-precision reflective volume Bragg gratings.

Reflecting Bragg gratings (RBGs) recorded in photo-thermo-refractive (PTR) glasses have been widely used in narrowing and stabilization of the laser emission spectrum. As the center wavelength of RBGs determines the final output wavelength of lasers, it is necessary to carefully control the center wavelength of RBGs during the fabrication process. In this paper, the fabrication process of high-precision RBGs was investigated. We developed a two-step method and demonstrated both theoretically and experimentally that it is effective and can be used to guide the fabrication process of high-precision RBGs. The experimental results show that the center wavelength of the fabricated RBG deviates from the target center wavelength within ±10 pm.

Continuous-wave laser damage mechanism of a spectral combining grating.

With increased power of spectral beam combination, surface heat distortion of multilayer dielectric gratings (MDGs) could occur. In this study, the damage morphology of MDGs was initially analyzed under a continuous-wave laser irradiation. Subsequently, the surface distortion and temperature rise of different MDGs were tested experimentally. The experimental results showed that the initial damage of MDGs was caused by the thermal stress. Further, the thermal stress of the multilayer dielectric films on the MDG surface was analyzed theoretically. The calculated results were in good agreement with the experimental results. The conclusions indicated that with the increase of the MDG surface temperature, the stress in the HfO2 layers initially reached the stress damage threshold of the dielectric films and, therefore, the damage occurred.

Design and fabrication of multiplexed volume Bragg gratings as angle amplifiers in high power beam scanning system.

In this study, the realization of multiplexed volume Bragg gratings (VBGs) working as angle amplifiers in high power beam scanning system is theoretically and experimentally investigated. The design of the multiplexed VBG for the working wavelength of 1064 nm is described. We propose a cascaded multiplexed VBGs scheme that consists of 12 grating channels. Three 4-channel multiplexed VBGs were fabricated inside photo-thermo-refractive (PTR) glasses by multiple exposures and subsequent heat treatment. The test results show that this angle amplifier can achieve discrete angle deflection ranging from -45° to + 45°. The relative diffraction efficiency of all the grating channels is more than 80% and is almost polarization independent.

Dependence of temperature and far-field beam quality on substrate thickness of a spectral beam combining grating with 13.4 kW/cm2laser irradiation.

In previous research, the thermal distortion and far-field beam quality of a spectral beam combining grating were analyzed by theory and experiment under the irradiation of a high-power continuous-wave laser. It was concluded that the thermal expansion of the substrate was the main cause of the grating distortion and decrease in the beam quality. However, there was no further study to determine a method to decrease the heat deposition on the grating surface and far-field beam quality factor, M2. In this paper, we theoretically simulate the influence of the substrate thickness on the temperature field distribution and far-field beam quality of a multilayer dielectric grating. An experimental setup is proposed to verify the theoretical calculations. The experimental results are in good agreement with the calculations. The conclusions indicate that the temperature rise of the grating and M2 are effectively reduced by increasing the thickness of the substrate.

Motionless and fast measurement technique for obtaining the spectral diffraction efficiencies of a grating.

The measurement of the spectral diffraction efficiencies of a diffraction grating is essential for improving the manufacturing technique and for assessing the grating's function in practical applications. The drawback of the currently popular measurement technique is its slow speed due to the hundreds of repetitions of two kinds of time-consuming mechanical movements during the measuring process (i.e., the rotation of the mechanical arm to capture the light beam and the mechanical variation of the output wavelength of the grating monochromator). This limitation greatly restricts the usage of this technique in dynamic measurement. In this manuscript, we present a motionless and fast measurement technique for obtaining the spectral diffraction efficiencies of a plane grating, effectively eliminating the aforementioned two kinds of mechanical movements. Herein, the proposed solution for removing the first kind of mechanical movement is tested, and the experimental result shows that the proposed method can be successfully used to measure the plane transmission grating's spectral diffraction efficiencies in the wavelength range of 550-750 nm. The method for eliminating the second kind of mechanical movement is not verified in this manuscript; however, we think that it is very straightforward and commercially available. We estimate that the spectral measurement can be achieved on a millisecond time scale by combining the two solutions. Our motionless and fast measuring technique will find broad applications in dynamic measurement environments and mass industrial testing.

Study of the key factors affecting temperature of spectral-beam-combination grating.

Spectral beam combination is a promising method for high-radiance lasers with a good beam quality. With the increase of the combination power, the temperature of the multilayer dielectric grating (MDG) unavoidably increases, leading to surface heat distortion of the MDG. In this study, the temperature field equation of the MDG is derived, and the key factors influencing the MDG temperature are investigated. Furthermore, experiments are performed to confirm the calculation results. The results reveal that the increase of the thickness of the substrate can improve the power tolerance of the MDG but delays the stable output of beam laser; use of a substrate material with a large thermal conductivity can greatly reduce the temperature of the MDG.