Research on speed sensorless control strategy of permanent magnet synchronous motor based on fuzzy super-twisting sliding mode observer.

Aiming at the problems of poor adjustment effect, weak anti-disturbance ability, and low robustness of the traditional sliding mode algorithm for permanent magnet synchronous motor speed sensorless, a permanent magnet synchronous motor speed observation method combining super-twisting sliding mode control algorithm and fuzzy control is proposed to accelerate the convergence speed of the system and improve the anti-disturbance ability. Fuzzy rules are used to solve the problem of obtaining the upper bound of the boundary function in the super-twisting algorithm. Moreover, the fuzzy algorithm is used to output the variable sliding mode gain instead of the fixed sliding mode coefficient to improve the system robustness and suppress the jitter. The simulation results show that the overshoot of the control system is 4%, the lag time is not more than 0.003 ms, the speed error is not more than 1%, and the response and adjustment time is not more than 0.02 s. The proposed control strategy improves the tracking accuracy and response speed of the system, suppresses the sliding mode chattering, and enhances the anti-interference ability of the system.

Myo10 tail is crucial for promoting long filopodia.

Filopodia are slender cellular protrusions containing parallel actin bundles involved in environmental sensing and signaling, cell adhesion and migration, and growth cone guidance and extension. Myosin 10 (Myo10), an unconventional actin-based motor protein, was reported to induce filopodial initiation with its motor domain. However, the roles of the multifunctional tail domain of Myo10 in filopodial formation and elongation remain elusive. Herein, we generated several constructs of Myo10-full-length Myo10, Myo10 with a truncated tail (Myo10 HMM), and Myo10 containing four mutations to disrupt its coiled-coil domain (Myo10 CC mutant). We found that the truncation of the tail domain decreased filopodial formation and filopodial length, while four mutations in the coiled-coil domain disrupted the motion of Myo10 toward filopodial tips and the elongation of filopodia. Furthermore, we found that filopodia elongated through multiple elongation cycles, which was supported by the Myo10 tail. These findings suggest that Myo10 tail is crucial for promoting long filopodia.

The simplest Criegee intermediate CH2OO reaction with dimethylamine and trimethylamine: kinetics and atmospheric implications.

We have used the OH laser-induced fluorescence (LIF) method to measure the kinetics of the simplest Criegee intermediate (CH2OO) reacting with two abundant amines in the atmosphere: dimethylamine ((CH3)2NH) and trimethylamine ((CH3)3N). Our experiments were conducted under pseudo-first-order approximation conditions. The rate coefficients we report are (2.15 ± 0.28) × 10-11 cm3 molecule-1 s-1 for (CH3)2NH at 298 K and 10 Torr, and (1.56 ± 0.23) × 10-12 cm3 molecule-1 s-1 for (CH3)3N at 298 K and 25 Torr with Ar as the bath gas. Both reactions exhibit a negative temperature dependence. The activation energy and pre-exponential factors derived from the Arrhenius equation were (-2.03 ± 0.26) kcal mol-1 and (6.89 ± 0.90) × 10-13 cm3 molecule-1 s-1 for (CH3)2NH, and (-1.60 ± 0.24) kcal mol-1 and (1.06 ± 0.16) × 10-13 cm3 molecule-1 s-1 for (CH3)3N. We propose that the electronegativity of the atom in the co-reactant attached to the C atom of CH2OO, in addition to the dissociation energy of the fragile covalent bonds with H atoms (H-X bond), plays an important role in the 1,2-insertion reactions. Under certain circumstances, the title reactions can contribute to the sink of amines and Criegee intermediates and to the formation of secondary organic aerosol (SOA).

Lattice relaxation effects on the collective resonance spectra of a finite dipole array.

Dielectric/plasmonic lattice relaxation spectroscopy is theoretically discussed in this work. A lattice relaxation effect generally occurs in nanocrystals, which means that from the bulk phase to the crystal surface, lattice parameters show a gradual shift. Here, lattice relaxation is introduced into finite polarizable point arrays or rod arrays as an adjusting tool, and its effect on lattice resonance extinction spectrum peaks is calculated. DDA (discrete dipole approximation) and FDTD (finite difference time domain) methods are applied. Different from an ideal infinite array, a finite array exhibits a broad, rippled extinction spectral peak. The application of an expanded/contracted lattice relaxation to the finite array can compress the ripple on one shoulder of the peak, as a cost, and the other shoulder of the peak gets more rippled, showing a "ripple transfer" effect. The strategy introduced in this work can contribute to the micro/nano optical measurement, on-chip adjustable optical cavity for OPOs (optical parameter oscillators)/lasers and controlling of fluorescence or hot-electron chemistry.

Kinetics of the Simplest Criegee Intermediate CH2OO Reaction with tert-Butylamine.

The kinetics of the simplest Criegee intermediate (CH2OO) reaction with tert-butylamine ((CH3)3CNH2) was studied under pseudo-first-order conditions with the OH laser-induced fluorescence (LIF) method at the temperature range of 283-318 K and the pressure range of 5-75 Torr. Our pressure-dependent measurement showed that at 5 Torr─the lowest pressure measured in the current experiment─this reaction was under the high-pressure limit condition. At 298 K, the reaction rate coefficient was measured to be (4.95 ± 0.64) × 10-12 cm3 molecule-1 s-1. The title reaction was observed to be negative temperature-dependent; the activation energy of (-2.82 ± 0.37) kcal mol-1 and the pre-exponential factor of (4.21 ± 0.55) × 10-14 cm3 molecule-1 s-1 were derived from the Arrhenius equation. The rate coefficient of the title reaction is slightly larger than (4.3 ± 0.5) × 10-12 cm3 molecule-1 s-1 of the CH2OO reaction with methylamine; the electron inductive effect and the steric hindrance effect might play a role in contributing to such difference.

Increasing lysine level improved methanol assimilation toward butyric acid production in Butyribacterium methylotrophicum.

BACKGROUND: Methanol, a promising non-food fermentation substrate, has gained increasing interest as an alternative feedstock to sugars for the bio-based production of value-added chemicals. Butyribacterium methylotrophicum, one of methylotrophic-acetogenic bacterium, is a promising host to assimilate methanol coupled with CO2 fixation for the production of organic acids, such as butyric acid. Although the methanol utilization pathway has been identified in B. methylotrophicum, little knowledge was currently known about its regulatory targets, limiting the rational engineering to improve methanol utilization. RESULTS: In this study, we found that methanol assimilation of B. methylotrophicum could be significantly improved when using corn steep liquor (CSL) as the co-substrate. The further investigation revealed that high level of lysine was responsible for enhanced methanol utilization. Through the transcriptome analysis, we proposed a potential mechanism by which lysine confers improved methylotrophy via modulating NikABCDE and FhuBCD transporters, both of which are involved in the uptake of cofactors essential for enzymes of methanol assimilation. The improved methylotrophy was also confirmed by overexpressing NikABCDE or FhuBCD operon. Finally, the de novo synthetic pathway of lysine was further engineered and the methanol utilization and butyric acid production of B. methylotrophicum were improved by 63.2% and 79.7%, respectively. After an optimization of cultivation medium, 3.69 g/L of butyric acid was finally achieved from methanol with a yield of 76.3%, the highest level reported to date. CONCLUSION: This study revealed a novel mechanism to regulate methanol assimilation by lysine in B. methylotrophicum and engineered it to improve methanol bioconversion to butyric acid, culminating in the synthesis of the highest butyric acid titer reported so far in B. methylotrophicum. What's more, our work represents a further advancement in the engineering of methylotrophic-acetogenic bacterium to improve C1-compound utilization.

Acoustic Attenuation and Dispersion in Fatty Tissues and Tissue Phantoms Influencing Ultrasound Biomedical Imaging.

The development of ultrasonic imaging techniques is optimized using artificial tissue phantoms before the practical applications. However, due to the strong attenuation and dispersion, accumulated fatty tissues can significantly impact the resolution and even feasibility of certain ultrasonic imaging modalities. An appropriate characterization of the acoustic properties on fatty phantoms can help the community to overcome the limitations. Some of the existing methods heavily overestimate attenuation coefficients by including the reflection loss and dispersion effects. Hence, in this study, we use numerical simulation-based comparison between two major attenuation measurement configurations. We further pointed out the pulse dispersion in viscoelastic tissue phantoms by simulations, which barely attracted attention in the existing studies. Using the selected attenuation and dispersion testing methods that were selected from the numerical simulation, we experimentally characterized the acoustic properties of common fatty tissue phantoms and compared the acoustic properties with the natural porcine fatty tissue samples. Furthermore, we selected one of the tissue phantoms to construct ultrasound imaging samples with some biomasses. With the known attenuation and dispersion of the tissue phantom, we showed the clarity enhancement of ultrasound imaging by signal post-processing to weaken the attenuation and dispersion effects.

Effects of steam and CO2 on gasification tar composition and evolution of aromatic compounds.

The removal of tar is conducive to improving the energy efficiency of downstream equipment and reducing the damage caused to it. In this study, a two-stage continuous feeding apparatus was developed to explore the yield and characteristics of tar produced from the co-gasification of microcrystalline cellulose (MCC) and polyethylene (PE) under separate and mixed atmospheres of steam and CO2. The tar yield can effectively reduce to 2.27 % when the steam and feedstock mass ratio (S/F) is 0.8. CO2 can partially substitute the steam in the gasification process, which can effectively promote a decrease in benzofuran. Furthermore, Gaussian software was employed to analyze the evolution mechanism of aromatic compounds. When the temperature is more than 800 °C, hydrogen consumption in the benzene cracking process is reduced, which is instrumental in improving the quality of syngas. Naphthalene is prone to form through the recombination of two cyclopentadienyls. Controlling the cyclization of cyclopentadienyls is a critical step in reducing the formation of polycyclic aromatic hydrocarbons. H and OH radicals are critical in phenol and benzofuran cracking, respectively. Although radicals act differently on specific aromatic compounds, the gasification effect of CO2 is less than that of steam because steam can provide both H and OH radicals, whereas CO2 needs to consume H radicals to generate OH radicals. The results provide beneficial guidance for controlling tar formation.

High-efficiency, high-repetition-rate cavity-dumped Q-switched Yb:YAG thin-disk laser based on a 72-pass pump module.

We demonstrated a compact cavity-dumped Q-switched Yb:YAG laser based on a 72-pass pump module. A highly stable nanosecond pulse laser with a duration of 18.38 ns was achieved at a repetition rate of 100 kHz, which is pumped by multimode laser diode stacks at 969 nm. The average output power of more than 150 W is delivered in a good output beam characterized by M2 = 1.53. The result shows that the pulse duration derived from simulation agrees well with the one retrieved from the experimental measurements. To the best of our knowledge, the 43.5% optical-optical efficiency is the highest reported to date from a cavity-dumped Q-switched Yb:YAG TDL (thin-disk laser).

Resonators with a continuously variable output coupling rate to enhance output performance of Yb:YAG thin-disk lasers.

Beam quality and average output power are two long-sought parameters of continuous-wave lasers. The operating characteristics of a Yb:YAG thin-disk laser based on a 72-pass pump module using output couplers with continuously variable coupling rates are reported. When the pump power is 450 W, the average power of more than 210 W is obtained, and the corresponding optical-optical efficiency is about 46.67%. The output beam is near diffraction limited with M2=1.20 and 1.18 on the horizontal and vertical directions respectively. The thin-disk laser maintains the beam quality near diffraction limited within the whole pump power range by continuously changing the coupling rate of the output coupler (the combination of thin film polarizer and quarter-wave plate) according to the incident pump power. The experimental results show that by continuously changing the coupling rate, combined with the dynamic change of thermal lens effect, the laser beam quality factor M2 can be reduced from 2.0 to 1.2, and the beam quality can be improved by about 40%. The improvement of and increase of average output power confirm that resonators with continuously variable coupling rate are a promising method for enhancing output performance of thin-disk lasers.

Numerical simulation of the magnetically gain-switched chemical oxygen-iodine laser.

During the operation of the magnetically gain-switched chemical oxygen-iodine laser (MGS-COIL), the transition intensity of hyperfine transition line 2-2 can exceed that of line 3-4, which is the dominant line at zero magnetic field. For this reason, a simulation model including both 3-4 and 2-2 transition lines is necessary to describe the mode buildup process in MGS-COIL. In this paper, we assume that 3-4 and 2-2 transition lines simultaneously oscillate in laser cavity. The propagation of optical field is calculated based on FFT. The required frequency, rise time and residual field of the magnetic gain-switch for a high-performance MGS-COIL are analyzed based on simulation results.

Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (Micropterus salmoides).

Largemouth bass is an important commercially farmed fish in China, but the rapid expansion of its breeding has resulted in increased incidence of diseases caused by bacteria, viruses and parasites. In this study, moribund largemouth bass containing ulcer foci on body surfaces indicated the most likely pathogens were iridovirus and rhabdovirus members and this was confirmed using a combination of immunohistochemistry, cell culture, electron microscopy and conserved gene sequence analysis. We identified that these fish had been co-infected with these viruses. We observed bullet-shaped virions (100−140 nm long and 50−100 nm in diameter) along with hexagonal virions with 140 nm diameters in cell culture inoculated with tissue homogenates. The viruses were plaque purified and a comparison of the highly conserved regions of the genome of these viruses indicated that they are most similar to largemouth bass virus (LMBV) and hybrid snakehead rhabdovirus (HSHRV), respectively. Regression infection experiments indicated fish mortalities for LMBV-FS2021 and HSHRV-MS2021 were 86.7 and 11.1%, respectively. While co-infection resulted in 93.3% mortality that was significantly (p < 0.05) higher than the single infections even though the viral loads differed by >100-fold. Overall, we simultaneously isolated and identified LMBV and a HSHRV-like virus from diseased largemouth bass, and our results can provide novel ideas for the prevention and treatment of combined virus infection especially in largemouth bass.

A multi modal approach to microstructure evolution and mechanical response of additive friction stir deposited AZ31B Mg alloy.

Current work explored solid-state additive manufacturing of AZ31B-Mg alloy using additive friction stir deposition. Samples with relative densities ≥ 99.4% were additively produced. Spatial and temporal evolution of temperature during additive friction stir deposition was predicted using multi-layer computational process model. Microstructural evolution in the additively fabricated samples was examined using electron back scatter diffraction and high-resolution transmission electron microscopy. Mechanical properties of the additive samples were evaluated by non-destructive effective bulk modulus elastography and destructive uni-axial tensile testing. Additively produced samples experienced evolution of predominantly basal texture on the top surface and a marginal increase in the grain size compared to feed stock. Transmission electron microscopy shed light on fine scale precipitation of Mg[Formula: see text]Al[Formula: see text] within feed stock and additive samples. The fraction of Mg[Formula: see text]Al[Formula: see text] reduced in the additively produced samples compared to feed stock. The bulk dynamic modulus of the additive samples was slightly lower than the feed stock. There was a [Formula: see text] 30 MPa reduction in 0.2% proof stress and a 10-30 MPa reduction in ultimate tensile strength for the additively produced samples compared to feed stock. The elongation of the additive samples was 4-10% lower than feed stock. Such a property response for additive friction stir deposited AZ31B-Mg alloy was realized through distinct thermokinetics driven multi-scale microstructure evolution.

Establishment and evaluation of qPCR and real-time recombinase-aided amplification assays for detection of largemouth bass ranavirus.

Largemouth bass ranavirus disease (LMBVD) caused by largemouth bass ranavirus (LMBV) has resulted in severe economic losses in the largemouth bass (Micropterus salmoides) farming industry in China. Early and accurate diagnosis is the key measure for the prevention and control of LMBVD. In this study, a quantitative polymerase chain reaction (qPCR) and a real-time recombinase-aided amplification (real-time RAA) assay were established for the detection of LMBV. The sensitivity and specificity of these two methods, and the efficacy for detection of LMBV from clinical samples were also evaluated. Results showed that the real-time RAA reaction was completed in <30 min at 39℃ with a detection limit of 58.3 copies, while qPCR reaction required 60 min with a detection limit of 5.8 copies. Both methods were specific for LMBV, where no cross-reactions observed with the other tested fish pathogens. Comparing the amplification results of both assays to the results obtained by virus isolation using 53 clinical tissue samples, results showed that the clinical sensitivity of real-time RAA and qPCR were 93.75% and 100% respectively, and the clinical specificity of both were 100%. Our results showed that qPCR is more suitable for quantitative analysis and accurate detection of LMBV in the laboratory, while real-time RAA is more suitable as a point-of-care diagnostic tool for on-site detection and screening of LMBV under farm conditions and in poorly equipped laboratories.

Simultaneous negative reflection and refraction and reverse-incident right-angle collimation of sound in a solid-fluid phononic crystal.

The square lattice phononic crystal (PnC) has been used extensively to demonstrate metamaterial effects. Here, positive and negative refraction and reflection are observed simultaneously due to the presence of Umklapp scattering of sound at the surface of PnC and square-like equifrequency contours (EFCs). It is found that a shift in the EFC of the third transmission band away from the center of the Brillouin zone results in an effectively inverted EFC. The overlap of the EFC of the second and third band produce quasimomentum-matching conditions that lead to multi-refringence phenomena from a single incident beam without the introduction of defects into the lattice. Additionally, the coupling of a near-normal incident wave to a propagating almost perpendicular Bloch mode is shown to lead to strong right-angle redirection and collimation of the incident acoustic beam. Each effect is demonstrated both numerically and experimentally for scattering of ultrasound at a 10-period PnC slab in water environment.

Thermally Tunable Acoustic Beam Splitter Based on Poly(vinyl alcohol) Poly(N-isopropylacrylamide) Hydrogel.

In this study, we demonstrated a thermally tunable acoustic beam splitter using a poly(vinyl alcohol) poly(N-isopropylacrylamide) hydrogel (PVA-pNIPAM). The nature of PVA-pNIPAM hydrogel offers exceptional temperature-dependent physical properties due to its phase transition around its lower critical solution temperature. The acoustic impedance of the hydrogel can be tuned below, above, or matched to that of water by changing the environmental temperature. An acoustic wave propagating in water can be split into transmitted and reflected components by the PVA-pNIPAM hydrogel slab on varying its angle of incidence. The intensity ratio between the reflected and the transmitted componence can be adjusted by tuning the temperature of the medium. The acoustic beam can be entirely reflected at a temperature corresponding to the matched impedance between hydrogel and water. The beam-splitting behavior was observed for acoustic waves from both a monochromatic wave and broadband pulse source. In addition, the phase of beam split pulses can be reversed by selecting the hydrogel's operating temperature.

A Review of Diagnostics Methodologies for Metal Additive Manufacturing Processes and Products.

Additive manufacturing technologies based on metal are evolving into an essential advanced manufacturing tool for constructing prototypes and parts that can lead to complex structures, dissimilar metal-based structures that cannot be constructed using conventional metallurgical techniques. Unlike traditional manufacturing processes, the metal AM processes are unreliable due to variable process parameters and a lack of conventionally acceptable evaluation methods. A thorough understanding of various diagnostic techniques is essential to improve the quality of additively manufactured products and provide reliable feedback on the manufacturing processes for improving the quality of the products. This review summarizes and discusses various ex-situ inspections and in-situ monitoring methods, including electron-based methods, thermal methods, acoustic methods, laser breakdown, and mechanical methods, for metal additive manufacturing.

Ultrasound Imaging by Thermally Tunable Phononic Crystal Lens.

This work demonstrates the detections and mappings of a solid object using a thermally tunable solid-state phononic crystal lens at low frequency for potential use in future long-distance detection. The phononic crystal lens is infiltrated with a polyvinyl alcohol-based poly n-isopropyl acrylamide (PVA-PNIPAm) bulk hydrogel polymer. The hydrogel undergoes a volumetric phase transition due to a temperature change leading to a temperature-dependent sound velocity and density. The temperature variation from 20 °C to 39 °C changes the focal length of the tunable solid-state lens by 1 cm in the axial direction. This thermo-reversible tunable focal length lens was used in a monostatic setup for one- and two-dimensional mapping scans in both frequency domain echo-intensity and temporal domain time-of-flight modes. The experimental results illustrated 1.03 ± 0.15λ and 2.35 ± 0.28λ on the lateral and axial minimum detectable object size. The experiments using the tunable lens demonstrate the capability to detect objects by changing the temperature in water without translating an object, source, or detector. The time-of-flight mode modality using the tunable solid-state phononic lens increases the signal-to-noise ratio compared to a conventional phononic crystal lens.

Effect of steam on the homogeneous conversion of tar contained from the co-pyrolysis of biomass and plastics.

The co-pyrolysis tar formed from microcrystalline cellulose (MCC) and polyethylene (PE) was used to study their further conversion path under the effect of steam. This paper addressed the yield and transformation of tar with different steam/feedstock mass ratios (S/F= 0.8, 1.6) in a two-stage fixed bed when the two stages' furnace temperature was set at 600°C and 800°C, separately. Compared with pyrolysis, steam promoted tar cracking effectively, the tar yield decreased at least 1/3. However, when the S/F ratio increases to 1.6, the cracking effect of tar is not further improved. The tar yield depended more on the PE content in the mixture, which was enhanced with PE increment. Besides, the H/C atom ratio was related to the conversion path of tar. Steam was beneficial to the cracking of compounds, but the generated hydrogen radicals affected the direction of the subsequent reaction. The steam mainly promotes the cracking of long-chain hydrocarbons, accompanied by cyclization and aromatization when the steam was limited. Nevertheless, the chain compounds are hard to crack efficiently when the steam was excessive, and the cyclization and aromatization processes were hindered due to the apparent effect of hydrogenation.

Bilateral retrocorneal hyaline scrolls secondary to asymptomatic congenital syphilis: A case report.

BACKGROUND: Retrocorneal hyaline scrolls are a rare phenomenon. We report a case of bilateral retrocorneal hyaline scrolls that were likely induced by asymptomatic congenital syphilis. CASE SUMMARY: A 71-year-old woman presented with blurred vision due to cataracts. Slit-lamp microscopy revealed bilateral hyaline scrolls with a dichotomous branching pattern extending to the anterior chamber or rods attaching to the rough posterior surface of the cornea. The patient was positive for syphilis-specific antibodies, with no ocular or systemic evidence of congenital or acquired syphilis. Binocular cataract, retrocorneal scroll, and corneal endothelial gutta were considered. The scroll of the right eye was removed during cataract surgery and further observed using hematoxylin-eosin staining and scanning electron microscopy. The cornea of the right eye remained transparent, and the residual scroll seemed stable, however, the corneal endothelial density declined at 13 mo after surgery. In vivo confocal microscopy revealed coalescence of corneal guttae at the level of the corneal endothelium or adhesion to the posterior surface of the endothelium, with enlarged endothelial cells in both eyes. Activated keratocytes in the stroma and a highly reflective acellular structure at the level of the Descemet's membrane were observed. The removed scroll had a cartilage-like hardness and a circularly arranged fiber-like acellular structure. CONCLUSION: Occult congenital syphilis could induce corneal endothelial gutta and the formation of retrocorneal scrolls without other signs of ocular syphilis.