1.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 29(1): 107-109, 1997.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12219245
RESUMO
We have designed and synthesized a hammer-head Rz targeted to human proliferating cell nuclear antigen (PCNA) mRNA at site No.524. Results showed that Rz524 can cleave the transcribed substrate RNA site-specifically in vitro. A self trimming expression plasmid of this Rz was constructed and co-introduced into HeLa cells with T7 vaccinia virus. Dot-blotting hybridization showed that the Rz was expressed in HeLa cells.