Protective effect of synthetic salidroside on acute lung injury in rats / 生理学报

To study the protective effect and mechanism of synthetic salidroside on acute lung injury (ALI) induced by lipopolysaccharide (LPS), male Sprague-Dawley (SD) rats were randomly divided into saline control group, 3 mg/kg LPS model group, different doses of salidroside groups (5, 20 and 80 mg/kg), and 5 mg/kg dexamethasone group. Intratracheal LPS instillation was used to establish the ALI model 0.5 h after intraperitoneal injection of salidroside or dexamethasone, and the rats were sacrificed 6 h later. Lung wet/dry weight ratio (W/D) was calculated. Lung tissue pathology and lung injury score (LIS) were observed and evaluated through hematoxylin and eosin (HE) staining. The centrifugal sediment of bronchoalveolar lavage fluid (BALF) was used to count the polymorphonuclear leukocyte (PMN) number by Wright's staining, and the centrifugal supernatant of BALF was used to determine the contents of protein and inflammatory factors (TNF-α, IL-1β and IL-6). The contents of myeloperoxidase (MPO) and malondialdehyde (MDA) in lung tissue were determined. Western blot was used to detect the expression levels of phosphorylated and total nuclear factor kappa B (NF-κB)/p65 protein in lung tissue. The results showed that, compared with LPS group, the intervention of synthetic salidroside alleviated the pathological damage in lung tissue, decreased the LIS and lung W/D ratio (P < 0.05), reduced the PMN number, the contents of protein and inflammatory factors in BALF (P < 0.05), reduced the contents of MPO and MDA in lung tissue (P < 0.05), and inhibited the expression of p-NF-κB in lung tissue (P < 0.05). The results suggest that synthetic salidroside has a protective effect on ALI induced by LPS, and its mechanism is related to inhibiting the phosphorylation of NF-κB and reducing the aggregation of PMN in the lung.

Association between gene polymorphism of CD40 gene and coronary artery lesion in Kawasaki disease / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the association between two single nucleotide polymorphisms (rs4810485 and rs1535045 in CD40 gene) and Kawasaki disease (KD) in Han Chinese children.</p><p><b>METHODS</b>A case-control study was performed on 184 children with KD and 206 normal controls. The polymorphisms of two SNPs in CD40 gene were detected using PCR-RFLP.</p><p><b>RESULTS</b>There were no significant differences in the genotype distribution and allele frequency of SNP rs4810485 in CD40 gene between the KD and normal groups (P>0.05). The genotype distribution of SNP rs1535045 in CD40 gene in the KD group was significantly different from the control group (P<0.05). T allele of SNP rs1535045 was shown as a risk factor for development of KD (OR=1.592, 95%CI: 1.182-2.144, P=0.004). There were no association between the polymorphisms of the two SNPs and coronary artery lesions (P>0.05).</p><p><b>CONCLUSIONS</b>SNP rs1535045 may be associated with the development of KD in Han Chinese children, while SNP rs4810485 may not.</p>

Analysis of risk factors for second replacement of left atrioventricular valve / 第二军医大学学报

Objective To assess the factors influencing the early and mid-term survival of patients receiving a second replacement of the left atrioventricular valve, so as to provide guidance for clinical practice. Methods We retrospectively analyzed 55 patients who underwent a second replacement of the left atrioventricular valve between Jan. 2005 and Dec. 2010 at Changhai Hospital. Their survival rate and its risk factors were analyzed. The control group included patients who received their first left atrioventricular valve replacement at the same hospital and had matched age, sex, and operation time. Univariate and multivariate Cox survival analyses were used to assess the survival risk factors at 30 days and 3 years after operation. Results The 30-day, 1-year, 3-year and 5-year survival rates of patients after reoperation were 92. 7%, 89. 1%,83. 6%, and 81.8%, respectively, which were not significantly different from those of the control group(94. 5%, 92. 7%, 90. 9%, and 87. 3%, respectively). The left ventricular ejection fraction was the only independent predictor of 30-day survival of patients after reoperation (P = 0. 018, RR= 15. 33). Diabetes mellitus and left ventricular ejection fraction were the independent predictors of 3-year survival (P = 0. 039,RR = 6. 242 and P = 0. 001,RR= 91. 957, respectively). Conclusion The early and mid-term mortality rates are not significantly different between the first and second replacement of left atrioventricular valve. Left ventricular ejection fraction is the only independent predictor of early-term survival after re-replacement of left atrioventricular valve; diabetes mellitus and left ventricular ejection fraction are the independent predictors of mid-term survival.

Preparation of the spin trapping probe, N-tert-butyl-alpha-phenylnitrone, nanoparticle and its affinity to hepatoma cells / 药学学报

This article describes the preparation of the N-tert-butyl-alpha-phenylnitrone (PBN) liposomes and their related characteristics. The PBN liposomes were prepared by film dispersion-supersonic method and the formula of liposomes was optimized by orthogonal uniform design. RP-HPLC was used to qualify the amount of PBN that entered into the hepatoma cells. Necrosis rate was also investigated by fluorescence activated cell sorter (FACS) after PBN liposomes transfection. Result showed that the mean particle size, entrapment efficiency, and polydispersity of the resulting PBN-liposome were 137.5 nm, 71.52% and 0.286, respectively. PBN liposomes can enter into the tumor cell stably and they have higher affinity to hepatoma cell compared with free PBN resulting in a higher necrosis rate after transfection. These results provide a potential method for early diagnosis and treatment of cancer using specific spin trapping probe targeting tumor cells.