Analysis on the Characteristics of Quality Variation of National Medical Device Supervision and Inspection in 2020 / 中国医疗器械杂志

This study is based on the summary of the characteristics of quality variation of national medical device supervision and inspection in 2020. According to the results of the national medical device supervision and inspection through comparative analysis, this study puts forward suggestions on the medical device production and supervision measures for the post-marketing products, so as to further improve the level of the medical device and ensure the safety use of medical device.

Naphthalene allyl trifluoromethyl benzocyclopentanone inhibits proliferation and induces apoptosis of lung cancer A549 cells in vitro / 南方医科大学学报

OBJECTIVE@#To investigate the molecular mechanism by which a novel naphthalene allyl trifluoromethyl benzocyclopentanone XX0335 inhibits the proliferation and induces apoptosis of lung cancer A549 cells.@*METHODS@#Lung cancer A549 cells were treated with 0.1% DMSO (control) or different concentrations (6.25, 12.5, and 25 μg/mL) of XX0335, and the changes in cell viability, cell cycle, proliferation and apoptosis were assessed with CCK-8 assay, EdU experiment, and flow cytometry. The effects of different concentrations of XX0335 on phosphorylation levels of proliferation-related proteins Akt, mTOR, Akt/mTOR and the expressions of cleaved PARP and cyclin D1 were determined using Western blotting. We also assessed the effect of XX0335 on tumor growth in a mouse model bearing A945 cell xenograft.@*RESULTS@#Treatment with XX0335 reduced the viability of A549 cells in a dose-dependent manner (P < 0.01) and significantly inhibited cell proliferation (P < 0.001). Flow cytometry showed that XX0335 treatment promoted apoptosis of the cells (P < 0.01) and caused an obvious increase of the number of G1-phase cells. Compared with DMSO, XX0335 significantly inhibited the phosphorylation of Akt and mTOR, increased the expression of cleaved PARP, and lowered the protein expression of cyclin D1. In the tumor-bearing mouse models, injection of XX0335 significantly decreased the tumor volume (P < 0.01).@*CONCLUSION@#XX0335 inhibits the proliferation, cycle and induces apoptosis of lung cancer A549 cells possibly by inhibiting the Akt/mTOR signal pathway.

Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells / 中国病理生理杂志

AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human nonsmall-cell lung cancer A549 cells.METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay.The apoptosis was analyzed by flow cytometry.The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of m TOR were determined by Western blot.RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L) , the cell viability was decreased (P<0.01) compared with DMSO control group.The apoptotic rate was increased compared with DMSO control group (P<0.01).The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of m TOR was inhibited (P<0.01).CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of m TOR.

Environment significance and hydrochemical characteristics of supra-permafrost water in the source region of the Yangtze River.

This paper analyzed the environment significance and hydrochemical characteristics of the main ions on the supra-permafrost water from the view of space-time and different ablations. The study is conducted through collected 330 samples from June to September in 2016 and 2017 in the Source Region of the Yangtze River (SRYR). The results showed the pattern of ionic dominance based on mean value was following as: Cl- > Na+ > SO42- > Ca2+ > Mg2+ > K+ > NO3- > NO2- > NH4+ > F- > Li+. Cl- alone contributed 40.57%, and Cl-, Na+, SO42- and Ca2+ accounted for 94.06% of the total ionic concentrations. The spatial variation of ionic concentration was larger at the initial stage of ablation, and more stable in strong ablation and the end of the ablation stage. The area with elevation of 4500 m was the region where the control source of ions was more sensitive to the hydrochemical characteristics of supra-permafrost water in the study area. Through the analysis of the sources of ions, the anions and cations in supra-permafrost water in the study area were mainly controlled by crustal sources including evaporates rocks and carbonate rocks. The recharge effect of precipitation and snow-melt water only affected the concentration of Cl-, NH4+, NO3-, F-, Li+ and K+, but did not affect NO2-, SO42-, Mg2+ and Ca2+. The hydrochemical type of supra-permafrost water was Ca2+-SO42- in high elevation (>4800 m). However, the hydrochemical type of middle elevation (from 4400 m to 4700 m) was Na+-Cl-. The type of hydrochemistry at low altitude was more complex than at high and middle elevation. This study provides insights on the construction of hydrological models in the cold regions and scientific basis for water resources management in the Tibet Plateaus.