RESUMO
Nine strains representing a single anamorphic novel yeast species in dispersed tropical and subtropical habitats were isolated from sugarcane leaf tissue (DMKU-SE24, DMKU-SE45T, DMKU-SE129 and DMKU-SE134) and corn leaf tissue (DMKU-CE36) in Thailand, phylloplane and rhizoplane of sugarcane in Brazil (IMUFRJ 52018 and IMUFRJ 52019), bromeliad leaf tissue in Brazil (IMUFRJ 51954) and plant leaf in Japan (IPM31-24). These strains showed identical or only 1 nt substitution in the sequences of the D1/D2 region of the LSU rRNA gene and 0-5 nt substitutions in the internal transcribed spacer (ITS) region. Phylogenetic analysis based on the combined sequences of the ITS and the D1/D2 regions showed that the eight of these strains represented a single species in the genus Occultifur that was distinct from related species. Occultifur kilbournensis was the most closely related species, but with 0.9-1.2â% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 2.4-2.6â% nucleotide substitutions in the ITS region. They are therefore considered to represent a novel species of the genus Occultifur although the formation of basidia was not observed. The name Occultifur plantarum f.a., sp. nov. is proposed. The type strain is DMKU-SE45T (=CBS 14554T=TBRC 6561T).
Assuntos
Basidiomycota/classificação , Filogenia , Saccharum/microbiologia , Zea mays/microbiologia , Composição de Bases , Basidiomycota/genética , Basidiomycota/isolamento & purificação , Brasil , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Japão , Técnicas de Tipagem Micológica , Folhas de Planta/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , TailândiaRESUMO
Five strains representing a single novel anamorphic yeast species were isolated from sugar cane. Two strains were from tissue (DMKU-SE38, DMKU-SE59(T)) and two from the external surface (DMKU-SP385, DMKU-SP403) of leaves collected in Thailand and the fifth (IMUFRJ 52020) from the rhizoplane of sugar cane in an organically cultivated field in Brazil. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were classified as representing a single species of the genus Occultifur. The sequences of the D1/D2 region of the LSU rRNA genes and the ITS regions of the five strains were either identical or differed from each other by only one nucleotide substitution. The novel species was related most closely to Occultifur brasiliensis f.a. CBS 12687(T) but with 0.7-1.0% nucleotide substitutions (4-6 nt) in the D1/D2 region of the LSU rRNA gene and 2.5-2.7% nucleotide substitutions (14-15 nt) in the ITS region. The name Occultifur tropicalis f.a., sp. nov. is proposed. The type strain is DMKU-SE59(T) (â=BCC 61184(T)â=NBRC 109696(T)â=CBS 13389 (T)).
Assuntos
Basidiomycota/classificação , Filogenia , Saccharum/microbiologia , Basidiomycota/genética , Basidiomycota/isolamento & purificação , Brasil , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Folhas de Planta/microbiologia , Análise de Sequência de DNA , Tailândia , Clima TropicalRESUMO
Two strains of a novel yeast species were isolated from insect frass and a flower in Thailand and Brazil, respectively. The strain from Thailand was isolated from insect frass collected in Than-Tong waterfall, Nong Khai Province, whereas the strain from Brazil was recovered from a flower of Ipomoea sp. collected on the banks of the Paraguai River in the state of Mato Grosso do Sul. The sequences of the D1/D2 domains of the large subunit of the rDNA of both strains were identical. This novel species belonged to the Metschnikowia clade and was related to Metschnikowia lunata. No signs of sporulation were observed for the two strains on various culture media. The novel species, Candida golubevii sp. nov., is proposed to accommodate these isolates. The type strain is BCC 8332(T) (=CBS 11362(T)=NBRC 105679(T)).
Assuntos
Candida/isolamento & purificação , Insetos/microbiologia , Ipomoea/microbiologia , Metschnikowia/isolamento & purificação , Animais , Candida/classificação , Candida/genética , Candida/fisiologia , DNA Fúngico/genética , DNA Ribossômico/genética , Flores/microbiologia , Metschnikowia/classificação , Metschnikowia/genética , Metschnikowia/metabolismo , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Reprodução AssexuadaRESUMO
Analyses of nucleotide sequences from the D1/D2 domains of the large-subunit rDNA and phenotypic characteristics showed that the genera Moniliella and Trichosporonoides are members of a single, monophyletic clade that would be best represented by a single anamorphic genus. On the basis of taxonomic priority, we propose the transfer of the five species of the genus Trichosporonoides to the genus Moniliella. The description of the genus Moniliella is emended and the following new combinations are proposed: Moniliella madida comb. nov., Moniliella megachiliensis comb. nov., Moniliella nigrescens comb. nov., Moniliella oedocephalis comb. nov. and Moniliella spathulata comb. nov. In addition, ten strains representing a novel yeast species belonging to the Moniliella clade were isolated from flowers in Thailand, Cuba and Brazil. Analysis of the internal transcribed spacer and D1/D2 large-subunit rDNA sequences indicated that the isolates represent a single species that was distinct from other species of the Moniliella clade. The name Moniliella fonsecae sp. nov. is proposed to accommodate these strains. The type strain is BCC 7726(T) (=CBS 10551(T)).