Characteristics of injury-induced death and potential years of life lost among residents in Changsha in 2014-2021 / 公共卫生与预防医学

Objective To investigate the injury death and its potential years of life lost among residents in Changsha from 2014 to 2021. Methods Data was obtained from the routine death cause monitoring records, and descriptive analysis was conducted using SPSS 21.0 to analyze the characteristics of injury death and life loss. Joinpoint software was applied to analyze and compare the mortality rates of injury death causes in different years. Results From 2014 to 2021, a total of 23862 deaths from injuries were reported in residents in Changsha, with an average annual crude mortality rate of 37.23/105 and an average standardized mortality rate of 33.82/105. The age standardized rate in the male was higher than that in the female (χ2＝2496.29，P=0.000). The standardized mortality rate of injury decreased year by year (APC=-4.013%，t=-3.052，P=0.022). The top five causes of death from injurie were traffic accident, accidental fall, drowning, suicide, and accidental poisoning. The leading causes of injury death were drowning in the age group of 0 to 14 years old, traffic accident in the group of 15 to 64 years old, and accidental fall in the group of 65 years old and above. From 2014 to 2021, the cumulative potential years of life lost (PYLL) of residents in Changsha was 398280.00 person years, the average years of life lost (AYLL) was 23.82 years per person, and the potential years of life lost rate (PYLLR) was 6.68‰. The top 5 types of injuries for PYLL were traffic accident, drowning, accidental fall, suicide, and accidental poisoning. Conclusion Injury is one of the important public health problems affecting the health of the residents in Changsha, and corresponding intervention measures should be taken according to the injury types of different populations.

The phenotypic changes of γδ T cells in COVID-19 patients

A novel pneumonia-associated respiratory syndrome named coronavirus disease-2019 (COVID-19), which caused by SARS-CoV-2 and broken in Wuhan, China in the end of 2019. Unfortunately, there is no specific antiviral agent or vaccine available to treat SARS-CoV-2 infections. Also, information regarding the immunological characteristics in COVID-19 patients remains limited. Here we collected the blood samples from 18 healthy donors (HD) and 38 COVID-19 patients to analyze changes in {gamma}{delta} T cells. In comparison to HD, the {gamma}{delta} T cells percentage was decreased. {gamma}{delta} T cells are able to immediately respond to SARS-CoV-2 infection and upregulate the activation marker CD25. In addition, the increased expression of CD4 in {gamma}{delta} T cells may serve as a biomarker for the assessment of SARS-CoV-2 infection.

Analysis of adaptive immune cell populations and phenotypes in the patients infected by SARS-CoV-2

Coronavirus disease-2019 (COVID-19), caused by SARS-CoV-2, has rapidly spread to most of countries in the world, threatening the health and lives of many people. Unfortunately, information regarding the immunological characteristics in COVID-19 patients remains limited. Here we collected the blood samples from 18 healthy donors (HD) and 38 COVID-19 patients to analyze changes in the adaptive immune cell populations and phenotypes. In comparison to HD, the lymphocyte percentage was slightly decreased, the percentages of CD4 and CD8 T cells in lymphocytes are similar, whereas B cell percentage increased in COVID-19 patients. T cells, especially CD8 T cells, showed an enhanced expression of late activation marker CD25 and exhaustion marker PD-1. Importantly, SARS-CoV-2 induced an increased percentage of T follicular helpher (Tfh)- and germinal center B-like (GCB-like) cells in the blood. However, the parameters in COVD-19 patients remained unchanged across various age groups. Therefore, we demonstrated that the T and B cells can be activated normally and exhibit functional features. These data provide a clue that the adaptive immunity in most people could be primed to induce a significant immune response against SARS-CoV-2 infection upon receiving standard medical care.

COVID-19 infection induces readily detectable morphological and inflammation-related phenotypic changes in peripheral blood monocytes, the severity of which correlate with patient outcome

BackgroundExcessive monocyte/macrophage activation with the development of a cytokine storm and subsequent acute lung injury, leading to acute respiratory distress syndrome (ARDS) is a feared consequence of infection with COVID-19. The ability to recognize and potentially intervene early in those patients at greatest risk of developing this complication could be of great clinical utility. MethodsWe performed detailed flow cytometric analysis of peripheral blood samples from 28 COVID-19 patients treated at Xian No.8 Hospital and the First Affiliated Hospital of Xian Jiaotong University in early 2020 in an attempt to identify factors that could help predict severity of disease and patient outcome. FindingsWhile we did not detect significant differences in the number of monocytes between patients with COVID-19 and normal healthy individuals,we did identify significant morphological and functional differences, which are more pronounced in patients requiring prolonged hospitalization and ICU admission. Patients with COVID-19 have larger than normal monocytes, easily identified on forward scatter, side scatter analysis by routine flow cytometry,with the presence of a distinct population of monocytes with high forward scatter (FSC-high). On more detailed analysis, these FSC-high monocytes are CD11b+, CD14+, CD16+, CD68+, CD80+, CD163+, CD206+ and secrete IL-6, IL-10 and TNF-alpha, consistent with an inflammatory phenotype. ConclusionsThe detection and serial monitoring of this subset of inflammatory monocytes using flow cytometry could be of great help in guiding the prognostication and treatment of patients with COVID-19 and merits further evaluation.

The microRNA of advanced colorectal cancer predict efifcacy of ifrst-line chemotherapy / 中国癌症杂志

Background and purpose: Colorectal cancer (CRC) is the most frequently occurring primary malignant tumor. Chemotherapy can reduce the risk of local and distant relapse. Therefore, it is very important to ifnd new biomarkers that can predict chemoresistant and help in treatment decisions. Methods:In this study, we examined the expression levels of 1 200 human miRNAs in 6 CRC tissues, using miRNA proifling assay arrays. A validation study was done to corroborate a subset of the results, including expression levels of miR-4299, miR-196b, miR-324-5p, miR-455-3p and miR-939, by analyzing 100 specimens of stageⅣcolorectal adenocarcinoma (not respond and respond to the chemotherapy) to quantitative real-time PCR. We modeled the relationship between the expression levels of these miRNAs and the survival rate of 100 CRC patients by Kaplan-Meier method. Results:Expression proifles in CRCs suggested that 5 miRNAs were candidate markers associated with the chemoresistance of colorectal cancer. We found that miR-4299 and-196b had signiifcant diagnostic value for chemoresistance CRC. miR-4299 yielded an AUC (the areas under the ROC curve) of 0.784 and miR-196b yielded an AUC of 0.647 in discriminating CRC from controls. Combined ROC analysis using these 2 miRNAs revealed an elevated AUC of 0.848 with 67.9%sensitivity and 90.9%speciifcity in discriminating chemoresistance CRC. The low level of miR-4299 expression and the high level of-196b expression are signiifcantly correlated with the good survival of CRC patients. Conclusion:These data suggest that miR-4299 and-196b have strong potential as novel biomarkers for chemoresistant detection of colorectal cancer.

Inhibitory effects of HSP27-siRNA on HCC cell lines / 中国普通外科杂志

Objective To detect the inhibitory effect of HSP27-siRNA on hepatocellular carcinoma(HCC) cells.Methods HCC cell QGY lines were cultured with HSP27-siRNA in a differtent range of concentration for various time periods.Cell activity was studied by MTT.The changes of cell cycle and apoptosis were analyzed by FCM.RT-PCR was used to detect the effect of HSP27-siRNA on QGY cell expression of HSP27 mRNA.Western blot was used to detect the inhibition efficiency of HSP27-siRNA on HSP27protein.Results The proliferation of QGY cell was inhibited by HSP27-siRNA,and HSP27-siRNA decreased the expression of HSP27 protein.HSP27-siRNA inhibited the proliferation of QGY cell line and induced apoptosis in vitro,and its effect was both dose-and time-dependent.Conclusions HSP27-siRNA can inhibit proliferation and induce apoptosis of HCC cancer cell lines.Thus,it may become a method for effective treatment of HCC cancer.