Optimization of Ultrasound-Assisted Pretreatment for Accelerating Rehydration of Adzuki Bean (Vigna angularis).

Adzuki bean (Vigna angularis), which provides plant-based proteins and functional substances, requires a long soaking time during processing, which limits its usefulness to industries and consumers. To improve this, ultrasonic treatment using high pressure and shear force was judged to be an appropriate pretreatment method. This study aimed to determine the optimal conditions of ultrasound treatment for the improved hydration of adzuki beans using the response surface methodology (RSM). Independent variables chosen to regulate the hydration process of the adzuki beans were the soaking time (2-14 h, X1), treatment intensity (150-750 W, X2), and treatment time (1-10 min, X3). Dependent variables chosen to assess the differences in the beans post-immersion were moisture content, water activity, and hardness. The optimal conditions for treatment deduced through RSM were a soaking time of 12.9 h, treatment intensity of 600 W, and treatment time of 8.65 min. In this optimal condition, the values predicted for the dependent variables were a moisture content of 58.32%, water activity of 0.9979 aw, and hardness of 14.63 N. Upon experimentation, the results obtained were a moisture content of 58.28 ± 0.56%, water activity of 0.9885 ± 0.0040 aw, and hardness of 13.01 ± 2.82 g, confirming results similar to the predicted values. Proper ultrasound treatment caused cracks in the hilum, which greatly affects the water absorption of adzuki beans, accelerating the rate of hydration. These results are expected to help determine economically efficient processing conditions for specific purposes, in addition to solving industrial problems associated with the low hydration rate of adzuki beans.

Delayed Quality Deterioration of Low-Moisture Cereal-Based Snack by Storing in an Active Filler-Embedded LDPE Zipper Bag.

This study focused on controlling the vapor permeability of an active zipper bag and preserving the quality of cereal-based snacks during the storage period at home. The active zipper bag was prepared by extruding low-density polyethylene with active fillers obtained from natural mineral materials. The active zipper bag showed the same transparent appearance as the existing one but showed 21% lower water vapor capability. As a result, during a 20-day storage period, three types of grain-based snacks (biscuits, shortbread cookies, and puffed snacks) showed delayed increases in weight, moisture content, and moisture activity when stored in an active zipper bag. In addition, this also affected the texture of the biscuits and shortbread cookies, in which the area under the curve was reduced significantly after appearing at a peak during the hardness measurement. On the other hand, the decrease in the number of air cell fracture events in puffed snacks was remarkable. This result suggests that the inner microstructure is preserved better when stored in an active zipper bag. In conclusion, the active zipper bag showed poor water vapor permeability, suggesting that the prepared zipper bag can be developed as snack packaging.

Visible on-site detection of Ara h 1 by the switchable-linker-mediated precipitation of gold nanoparticles.

Biosensors have been widely applied in tests for allergens, but on-site detection remains a challenge. Herein, we proposed a detection procedure for peanut Ara h 1 as a representative allergen, which was extracted from a cookie, thereby minimising the need for any complex pretreatment that was difficult to perform, and enabling the visual detection of the target without the use of analytical equipment. The extraction procedure was performed in less than 30 min using a syringe and filter (0.45 µm). The detection method for Ara h 1 was based on the aggregation of switchable linkers (SL) and gold nanoparticles (AuNP), and the presence of 0.19 mg peanut protein per 30 g of cookie could be confirmed within 30 min based on the AuNP/SL concentration ratio by the precipitation. This proposed procedure could be successfully applied to the detection of a wide range of food allergens.

Inactivation of Bacillus cereus ATCC 14579 Spore on Garlic with Combination Treatments of Germinant Compounds and Superheated Steam.

HIGHLIGHTS: Germinant compounds were combined with SHS to inactivate B. cereus spores. B. cereus spores were inactivated effectively by SHS after Ala+IMP treatment. Inactivation trend was analyzed by the Weibull model, and t3 d values were determined. Sublethal injury was not observed by SHS after Ala+IMP treatment.

The possible presence of natural ß-D-glucosidase inhibitors in jujube leaf extract.

Isoquercitrin is a phenolic compound well-known for having greater health benefits than quercitin, its aglycone derivative, and other related glycosides. However, isoquercitrin is rarely found in nature. Here, we optimized the conditions for the enzymatic transformation of isoquercitrin from rutin that was extracted from jujube leaf using the hesperidinase, enzyme complex containing ß-D-glucosidase and α-L-rhamnosidase. The maximum productivity (2.57±0.16mg/mL) was experimentally found under the following conditions: 47.3°C, 52.16h, and pH 5.31, which agreed well with the predicted value (2.65mg/mL). However, the achievement of this maximum yield was due to the absence of ß-D-glucosidase activity. Further investigations using a ß-D-glucosidase assay and reaction measurements under various conditions revealed that the ß-D-glucosidase activity was not blocked by denaturation or known inhibitory factors. Currently, there are no recognized ß-D-glucosidase inhibitors present in the jujube leaf; however, our observations strongly suggest that an unidentified ß-D-glucosidase inhibitor exists in jujube leaf extract.