RESUMO
Interleukin 1 (IL-1) exerts biological properties on various immune and nonimmune cell types and tissues and thus may play an important role during chronic inflammatory processes. Here we have examined the IL-1 biosynthesis in adherent synovial lining cell (ASLC) cultures obtained from patients with rheumatoid arthritis (RA). We report that ASLCs in culture showed heterogeneous endogenous levels of IL-1 alpha and beta expression. Recombinant interleukin 1 (rIL-1) alpha or beta induced increases of IL-1 alpha and beta mRNA and proteins levels in ASLCs. Although IL-1 synthesis is enhanced by rIL-1 treatment, no soluble IL-1 alpha or beta could be detected by specific enzyme-linked immunosorbent assays. A pretreatment with recombinant IFN gamma (rIFN gamma) down-regulated the effect of rIL-1 on IL-1 synthesis in ASLCs. Actinomycin D suppressed the endogeneous and rIL-1-induced IL-1 mRNA expression Indomethacin, in the presence of rIL-1 alpha or beta, up-regulates the level of expression of IL-1 beta in ASLCs pretreated with rIFN gamma, but has the opposite effect in non-pretreated cells. The increase of IL-1 gene expression by rIL-1 in human ASLCs from RA patients may contribute as an amplification of the disease progress. These studies may also explain the beneficial effects of IFN gamma in experimental models of IL-1-induced bone and cartilage degradation and in patients with diseases involving IL-1.
Assuntos
Artrite Reumatoide/imunologia , Interferon gama/farmacologia , Interleucina-1/biossíntese , Anti-Inflamatórios não Esteroides/farmacologia , Células Cultivadas , Regulação para Baixo , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Expressão Gênica , Humanos , Indometacina/farmacologia , Interleucina-1/genética , Interleucina-1/farmacologia , RNA Mensageiro , Proteínas Recombinantes , Membrana Sinovial/citologiaRESUMO
Lymphoid cells were isolated from normal human mucosal specimens. A certain fraction of these cells exhibited double antibody specificities at the levels of both light kappa and lambda chains and heavy mu and gamma chains. The variable frequency with which these cells were detected appears to be a characteristic of this population. They represent a very low proportion of the total number of cells isolated.
Assuntos
Mucosa Intestinal/imunologia , Linfócitos/imunologia , Especificidade de Anticorpos , Imunofluorescência , Humanos , Cadeias Pesadas de Imunoglobulinas , Cadeias Leves de Imunoglobulina , Técnicas In Vitro , Mucosa Intestinal/citologiaRESUMO
The presence of single human lymphoid cells expressing on the one hand both kappa and lambda light chain and on the other hand either both mu and alpha or both mu and gamma heavy chains, was observed in the intestinal mucosa. The variability of the frequency of such cells, both from one subject to another, or in the same sample, is a characteristic of this population.