[The expression profile of Arabidopsis thaliana ß-1,3-glucanase promoter in tobacco].

Well characterized promoters with specific activity only during male gametophyte development of transgenic organism are widely utilized in strategies aimed at the elimination of unwanted transgene escape from the transgenic to the non-transgenic plant population. Since the specificity and timing of the applied promoter in the original and transgenic organism don't have to be consistent, here we have tested by promoter-GUS fusion analysis the APRS promoter isolated from Arabidopsis thaliana in transgenic tobacco plants. Unlike of A. thaliana transcriptomic microarray data that identified gene expression from this promoter in late stages of pollen development, in tobacco plants the APRS promoter was active in the developing microspores during a short period of time before the microspores are released from the tetrads. Despite these discrepancies, the APRS promoter remains strictly pollen specific in tobacco plants. However, verification of its specificity in other important crops should precede the use of the APRS promoter in the engineered male sterility or in production of transgene-free pollen via site-specific recombination systems.

Core promoters of the penicillin biosynthesis genes and quantitative RT-PCR analysis of these genes in high and low production strain of Penicillium chrysogenum.

The transcription start points of the penicillin biosynthesis genes from Penicillium chrysogenum were mapped using the primer extension method. For each of the three genes consensus sequences of the core promoter elements were identified, supporting the notion that the basal transcription of these genes is mediated separately. Interestingly, transcription start of the pcbC gene is located within the potential Inr element with no TATA box-like sequence being found at expected position. This is in contrast to pcbAB and penDE genes with proposed TATA boxes or even to Aspergillus nidulans ipnA (pcbC) gene indicating possible differences in basal transcription regulation. Using the quantitative RT-PCR analysis the expression of all three biosynthesis genes was monitored in both the high and low production strain of P. chrysogenum during a 3-d cultivation under production conditions. The differences were found between the strains in time regulation and transcript levels of the biosynthesis genes. Furthermore, we showed that the effect of higher gene dosage on productivity in the production strain is amplified by more efficient transcription of the biosynthesis genes with the RNA levels approximately 37- and 12-times higher, respectively, than in a low production strain.